Abstract

Doc number: 78

Abstract

Background: The transcription factor SRF (serum response factor) mediates neuronal survival in vitro . However, data available so far suggest that SRF is largely dispensable for neuron survival during physiological brain function.

Findings: Here, we demonstrate that upon neuronal injury, that is facial nerve transection, constitutively-active SRF-VP16 enhances motorneuron survival. SRF-VP16 suppressed active caspase 3 abundance in vitro and enhanced neuron survival upon camptothecin induced apoptosis. Following nerve fiber injury in vitro , SRF-VP16 improved survival of neurons and re-growth of severed neurites. Further, SRF-VP16 enhanced immune responses (that is microglia and T cell activation) associated with neuronal injury in vivo. Genome-wide transcriptomics identified target genes associated with axonal injury and modulated by SRF-VP16.

Conclusion: In sum, this is a first report describing a neuronal injury-related survival function for SRF.

Details

Title
Serum response factor modulates neuron survival during peripheral axon injury
Author
Stern, Sina; Sinske, Daniela; Knöll, Bernd
Pages
78
Publication year
2012
Publication date
2012
Publisher
BioMed Central
e-ISSN
1742-2094
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1742-2094-9-78
ProQuest document ID
1029863663
Copyright
© 2012 Stern et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.