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Biochem Genet (2012) 50:797808
DOI 10.1007/s10528-012-9521-5
V. Sundaresan G. Sahni R. S. Verma
R. C. Padalia S. Mehrotra Sanjog T. Thul
Received: 19 September 2011 / Accepted: 2 March 2012 / Published online: 23 June 2012 Springer Science+Business Media, LLC 2012
Abstract An effort was made to determine the impact of geographic range on genetic richness and chemical constituents of Valeriana jatamansi Jones, an herb indigenous to the northwestern Himalaya. The genetic structure of 16 accessions from two major divisions of Uttarakhand state (Kumaon and Garhwal) was analyzed by ISSR markers. Overall genetic diversity among the populations was 45 %, with a cumulative range of 3592 % similarity for most of the high-altitude plants and a comparatively narrow range, 5088 %, for the population below the altitude of 1,800 m. Likewise, a remarkable predictability was evident from the chemical constituents on an individual basis. In principal component analysis, most of the accessions fall into two major groups and are classied as chemotypes based on the percentage of similar chemical constituents; these are mostly correlated to altitude. Geographic distance seems to inuence the genetic and chemical variability, indicating the genetic inbreeding within the population.
Keywords Terpenoid proling Patchouli alcohol Principal component
analysis ISSR markers Genetic cluster analysis
V. Sundaresan G. Sahni
Taxonomy and Pharmacognosy, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Kukrail Picnic Road, P.O. CIMAP, Lucknow 226015, India
R. S. Verma R. C. Padalia
CIMAP Research Centre, P.O. Dairy Farm, Nagla, Pantnagar, 263149 Uttarakhand, India
S. Mehrotra S. T. Thul (&)
Plant Biotechnology, CSIR-CIMAP, Kukrail Picnic Road, P.O. CIMAP, Lucknow 226015, India e-mail: [email protected]
Impact of Geographic Range on Genetic and Chemical Diversity of Indian Valerian (Valeriana jatamansi) from Northwestern Himalaya
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Introduction
The genus Valeriana belongs to the Valerianaceae, a family of mostly perennial herbs. Leaves and roots of the members have an aromatic smell. Valerian is used for insomnia and other disorders as an alternative to benzodiazepine drugs. It is also used as a sedative for nervous tension, hysteria, excitability, stress, and intestinal colic or cramps. Members of the genus are in great demand in the medicinal and perfumery markets, which has contributed to the groups status as threatened or endangered in the wild. In India, 16...