Content area
Full Text
Curr Microbiol (2013) 66:1621
DOI 10.1007/s00284-012-0200-x
Polaribacter reichenbachii sp. nov.: A New Marine Bacterium Associated with the Green Alga Ulva fenestrata
Olga I. Nedashkovskaya Andrey D. Kukhlevskiy
Natalia V. Zhukova
Received: 16 April 2012 / Accepted: 3 July 2012 / Published online: 28 September 2012 Springer Science+Business Media, LLC 2012
Abstract A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 6Alg 8T, was isolated from the common Pacic green alga Ulva fenestrata. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 97.6 % to Polaribacter dokdonensis DSW-5T and 92.896.1 % to other recognized Polaribacter species. The prevalent fatty acids of strain 6Alg 8T were iso-C15:0, iso-C15:1, iso-C15:0 2-OH, C15:0 and
C15:1x6. The polar lipid prole consisted of the major
lipids phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The DNA G?C content of the type strain is 31.6 mol%. The new isolate and the type strains of recognized species of the genus Polaribacter
were readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Polaribacter, for which the name Polaribacter reichenbachii sp. nov. is proposed. The type strain is 6Alg 8T (= KCTC 23969T = KMM 6386T = LMG 26443T).
Introduction
The genus Polaribacter, a member of the family Flavobacteriaceae, was originally proposed by Gosink et al. [9] to accommodate aerobic, pigmented, psychrophilic and psychrotrophic bacteria characterized by a bipolar distribution [26]. At the time of writing, the genus Polaribacter comprises seven species with validly published names [9, 15, 21, 30]. Species of the genus Polaribacter were rst found in sea and lake waters and sea ice samples were collected in Antarctica and Arctic [9]. It is interesting that strains of Polaribacter glomeratus, formerly Flectobacillus glomeratus, were recovered from waters of both the Burton Lake and the Prydz Bay located in Antarctica [18]. Later, three recognized Polaribacter species were isolated from water samples in Sea of Japan [15, 21, 30]. The recent results indicated that bacteria afliated with the genus Polaribacter are widely distributed in the different marine environments. These bacteria are...