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Acta Neuropathol (2013) 125:215229 DOI 10.1007/s00401-012-1042-0
ORIGINAL PAPER
Neurochemical mapping of the human hippocampus reveals perisynaptic matrix around functional synapsesin Alzheimers disease
Dvid Lendvai Markus Morawski Lszl Ngyessy Georgina Gti
Carsten Jager Gbor Baksa Tibor Glasz Johannes Attems
Heikki Tanila Thomas Arendt Tibor Harkany Aln Alpr
Received: 26 June 2012 / Revised: 24 August 2012 / Accepted: 27 August 2012 / Published online: 9 September 2012 Springer-Verlag 2012
Abstract Perineuronal matrix is an extracellular protein scaffold to shape neuronal responsiveness and survival. Whilst perineuronal nets engulf the somatodendritic axis of neurons, axonal coats are focal extracellular protein aggregates surrounding individual synapses. Here, we addressed the chemical identity and subcellular localization of both perineuronal and perisynaptic matrices in the human hippocampus, whose neuronal circuitry is progressively
compromised in Alzheimers disease. We hypothesized that(1) the cellular expression sites of chondroitin sulphate proteoglycan-containing extracellular matrix associate with specic neuronal identities, reecting network dynamics, and (2) the regional distribution and molecular composition of axonal coats must withstand Alzheimers disease-related modications to protect functional synapses. We show by epitope-specic antibodies that the perineuronal protomap of the human hippocampus is distinct from other mammals since pyramidal cells but not calretinin? and calbindin? interneurons, neurochemically classied as novel neuronal subtypes, lack perineuronal nets. We nd that cartilage link protein-1 and brevican-containing matrices form isolated perisynaptic coats, engulng both inhibitory and excitatory
D. Lendvai and M. Morawski contributed equally to the present study.
Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s00401-012-1042-0
Web End =10.1007/s00401-012-1042-0 ) contains supplementary material, which is available to authorized users.
D. Lendvai G. Gti G. Baksa A. Alpr
Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary
M. Morawski C. Jager T. Arendt (&)
Paul Flechsig Institute for Brain Research, Faculty of Medicine, Universitat Leipzig, Leipzig, Germanye-mail: [email protected]
M. MorawskiDepartment of Neuroscience and Physiology, SUNY Upstate Medical University, WH 3240, 750 East Adams Street, Syracuse, NY 13210, USA
L. NgyessyDepartment of Theory, Wigner Research Centre for Physics, Institute for Particle and Nuclear Physics, Hungarian Academy of Sciences, Budapest, Hungary
T. GlaszSecond Department of Pathology, Semmelweis University, Budapest, Hungary
J. AttemsInstitute for Ageing and Health, Newcastle University, Newcastle upon Tyne, UK
H. TanilaA.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland
H. TanilaDepartment of Neurology, Kuopio University...