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Abstract
A simple, sensitive and accurate spectrophotometric method has been developed for the determination of Acebrophylline in bulk drug and capsule. The λ max of the Acebrophyllline was found to be 274 nm. The method showed high sensitivity with linearity in the range of 2 to 20µg/ml, coefficient regression was found to be 0.9994. The regression of the curve was found to be Y = 0.0187X - 0.0062. Percent relative standard deviation value is below 2.0 for intraday and interday precision indicated that method is highly precised. The percentage recovery value (average 100.1458 percentages), indicated the accuracy and specificity of the method. The proposed method will be suitable for the analysis of Acebrophylline in bulk and pharmaceutical formulation.
Key Words
Acebrophylline, UV visible spectrophotometer.
Introduction
Acebrophylline,1,2,3,6-tetrahydro-1,3-dimethyl-2,6-dioxo-7H-Purine-7-aceticacid compound with trans-4-[[(2-amino-3,5 dibromophenyl)methyl] amino] cyclohexanol is used in bronchial asthma and pulmonary diseases1. Literature survey reveals that very few methods are available for estimation of Acebrophylline in bulk drug and its pharmaceutical formulation by UV spectrophotometry2. A new UV spectrophotometric method based on single wavelength analysis3 of Acebrophylline at max 274 nm was done.
Chemicals & reagents
Acebrophylline reference standard was kindly provided by AMI LIFESCIENCE PVT. LTD.82/B, ECP, Canal Rd.Karkhadi, Padra, Baroda. Analytical grade ethanol was purchased from Merck Chemicals, India.
Instrument
All the measurements were made using JASCO UV- V-550 UV Visible Spectrophotometer with 1 cm matched quartz cells.
Methods
Selection of wavelength
In order to ascertain the wavelength of maximum absorption (λmax) of the drug, different solutions of the drugs (10µg/ml) in ethanol were scanned using spectrophotometer within the wavelength...