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Theor Appl Genet (2013) 126:963970 DOI 10.1007/s00122-012-2029-x
ORIGINAL PAPER
Discovery and identication of a novel Ligon lintless-like mutant(Lix) similar to the Ligon lintless (Li1) in allotetraploid cotton
Caiping Cai Xiangchao Tong Fengju Liu
Fenni Lv Haihai Wang Tianzhen Zhang
Wangzhen Guo
Received: 21 October 2011 / Accepted: 6 December 2012 / Published online: 9 February 2013 Springer-Verlag Berlin Heidelberg 2013
Abstract Mutants are a powerful resource for studying gene structure, function, and evolution. In this present study, a novel Ligon lintless-like mutant (Lix), that has short bers and deformed leaves and stems, was isolated from the progeny of transgenic cottons. The Lix mutant is similar in morphology to the Ligon lintless (Li1) mutant.
Genetic analysis and molecular mapping were performed for the Lix and Li1 mutants. These two mutants are monogenic dominant mutants with distorted growth of vegetative and reproductive structures. Seedlings of the dominant homozygote Li1Li1 genotype are lethal, while
LixLix plants are viable but show no reproductive growth.
Molecular tagging showed that the Lix gene is located on Chr. 04 in a 30.9-cM region spanned by NAU8376 and NAU3469. In a previous study, the Li1 gene was mapped to
Chr. 22, and Chr. 04 and Chr. 22 are homoelogous chromosomes in tetraploid cotton. So, we propose that Lix and Li1 mutants have similar mutated morphology, and Lix is mapped to a homoelogous chromosome carrying Li1. The
identication and genetic mapping of Lix/Li1 genes using mutants provides a foundation for isolating these genes. In turn, this will permit studies to elucidate the functional and evolutionary roles for these genes in cotton growth and development.
Introduction
Cotton (Gossypium spp.) is the worlds most important natural textile ber and a signicant oilseed crop. Tens of thousands of genes are expressed during cotton development. Mutants can provide insight for elucidating the genetic factors that are critical for controlling a given phenotype. Thus, mutants are a powerful resource for studying gene structure, function, and evolution. To date, there have been many mutants reported in cotton including open-bud (ob1, ob2), virescent (vl), sterile-dwarf (sda), restores fertility (Rf), and nitrate nonutilizing gene (nit) (Endrizzi and Nelson 1989; Endrizzi and Ray 1991; Qian et al. 2009; Killough and Horlacher 1933; Wu et al. 2009; Yin et al. 2006; Korolev et al....