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Abstract
A Gram-negative, non-spore-forming, catalase- and oxidase-positive, aerobic, curved-rod shaped bacterium with polar or subpolar flagellum, designated strain JYr2^sup T^, was isolated from a sediment sample collected from an amphioxus breeding zone in the coastal region of Qingdao, China. The organism grew optimally at 37 °C, pH 8-9 and in the presence of 3 % (w/v) NaCl or 3-4 % sea salts (w/v; Sigma). Salt was required for growth. The strain contained isoprenoid quinone 8 (Q-8, 100 %) as the predominant isoprenoid quinone and C^sub 16:0^ (24.1 %) and C^sub 16:1^ [omega]7c and/or C^sub 16:1^ [omega]6c (35.8 %) as major fatty acids. Phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid (PN) and two aminolipid (AL1, AL2) were the major constituents of the phospholipids. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JYr2^sup T^ formed a distinct evolutionary lineage within the family Colwelliaceae. It showed <95.0 % sequence similarities to all validly published species in the family Colwelliaceae, except 95.2 % to Thalassomonas viridans DSM 13754^sup T^ and 95.0 % to Colwellia polaris JCM 537^sup T^. The G+C content of the DNA was 42.7 mol%. On the basis of the polyphasic taxonomic study, strain JYr2^sup T^ (= CGMCC 1.10794^sup T^ = JCM 17549^sup T^) was considered to represent a novel genus and species in Gammaproteobacteria, for which the name Litorilituus sediminis gen. nov. sp. nov. was proposed.[PUBLICATION ABSTRACT]