Content area
Full Text
Bulletin of Experimental Biology and Medicine, Vol. 149, No. 5, 2010 GENERAL PATHOLOGY AND PATHOPHYSIOLOGY
575
Accumulation of Calcium, Phosphate, and Collagen in Bones and Accumulation of Creatine in Muscles of Mice with Acute Hepatic Intoxicationduring Shin Fracture Healing
M. V. Stogov, S. N. Luniova, and E. A. Tkachuk
Translated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 149, No. 5, pp. 507-509, May, 2010 Original article submitted August 14, 2009
The intensity of calcium and phosphate accumulation in regenerating bone tissue decreased signicantly during the posttraumatic period in 54 male CBA mice with acute hepatic intoxication. The formation of organic components of the bone decreased to a lesser degree. Creatine metabolism in the muscles of damaged segment virtually did not change.
Key Words: trauma; acute hepatic intoxication; muscle; bone
The signicance of the liver for bone and muscle tissue metabolism is systemic. The liver regulates the distribution of energy substrates between the organs (and synthesizes creatine), is involved in calcium-phosphorus metabolism [4], produces growth factors which have anabolic effects on the skeletal muscles [9], on osteosynthesis and osteolysis processes [3,5]. Chronic hepatic diseases lead to growth inhibition in children and to osteopenia and osteoporosis in adults [6,8] and hence, to a higher incidence of limb bone fractures in this patient population [7]. This gave grounds to speak about the so-called hepatic osteodystrophy (or osteopathy) [2,11], its pathogenesis being attributed to reduced protein producing function of the liver [10]. However, it remains unclear to what degree the reduction of the synthetic function of the organ under conditions of acute hepatic insufciency (AHI) is essential for tissue reparation processes after skeletal injury.
We studied accumulation of calcium, phosphate, and collagen in the bones and of creatine in the skeletal muscles after skeletal injury in mice with acute hepatic intoxication.
MATERIALS AND METHODS
Experiments were carried out on 54 male CBA mice (25-30 g). The animals were distributed into 3 groups. In group 1 (n=18), closed fracture of the shin bone was modeled (the segment of the right hind limb was broken in ether-narcotized animals). In group 2 (n=18), AHI was induced by a single intraperitoneal injection of 20% carbon tetrachloride (CTC) in olive oil in a dose of 0.2 ml/100 g. Morphological studies carried out in...