An entanglement-enhanced microscope

Abstract

Among the applications of optical phase measurement, the differential interference contrast microscope is widely used for the evaluation of opaque materials or biological tissues. However, the signal-to-noise ratio for a given light intensity is limited by the standard quantum limit, which is critical for measurements where the probe light intensity is limited to avoid damaging the sample. The standard quantum limit can only be beaten by using N quantum correlated particles, with an improvement factor of [SQRT]N. Here we report the demonstration of an entanglement-enhanced microscope, which is a confocal-type differential interference contrast microscope where an entangled photon pair (N=2) source is used for illumination. An image of a Q shape carved in relief on the glass surface is obtained with better visibility than with a classical light source. The signal-to-noise ratio is 1.35±0.12 times better than that limited by the standard quantum limit.

ARTICLE

Received 11 Apr 2013 | Accepted 9 Aug 2013 | Published 12 Sep 2013

Among the applications of optical phase measurement, the differential interference contrast microscope is widely used for the evaluation of opaque materials or biological tissues. However, the signal-to-noise ratio for a given light intensity is limited by the standard quantum limit, which is critical for measurements where the probe light intensity is limited to avoid damaging the sample. The standard quantum limit can only be beaten by using N quantum correlated particles, with an improvement factor of N

p . Here we report the demonstration of an entanglement-enhanced microscope, which is a confocal-type differential interference contrast microscope where an entangled photon pair (N 2) source is

used for illumination. An image of a Q shape carved in relief on the glass surface is obtained with better visibility than with a classical light source. The signal-to-noise ratio is 1.350.12 times better than that limited by the standard quantum limit.

DOI: 10.1038/ncomms3426

An entanglement-enhanced microscope

Takafumi Ono1,2, Ryo Okamoto1,2 & Shigeki Takeuchi1,2

1 Research Institute for Electronic Science, Hokkaido University, N20W10, Kita-Ward Sapporo 001 0020, Japan. 2 The Institute of Scientic and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567 0047, Japan. Correspondence and requests for materials should be addressed to S.T. (email: mailto:[email protected]

Web End [email protected] ).

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Quantum metrology involves using quantum mechanics to realize more precise measurements than those that can be achieved classically1. The canonical example uses

entanglement of N particles to measure a phase with a precision Df 1/N, known as the Heisenberg limit. Such a measurement

outperforms the Df 1=

N

a b

Laser Polarizer

Sample

NOON state

Phase shift: N[p10]

Polarization

beam

splitter

p precision limit possible with N unentangled particlesthe standard quantum limit (SQL). Progress has been made with trapped ions24 and atoms5, whereas high-precision optical phase measurements have many important applications, including microscopy, gravity wave detection, measurements of material properties, and medical and biological sensing. Recently, the SQL has been beaten with two photons610 and four photons1113.

Perhaps the next natural step is to demonstrate entanglement-enhanced metrology1416. Among the applications of optical phase measurement, microscopy is essential in broad areas of science from physics to biology. The differential interference contrast microscope (DIM)17 is widely used for the evaluation of opaque materials or the label-free sensing of biological tissues18. For instance, the growth of ice crystals has recently been observed with a single molecular step resolution using a laser confocal microscope (LCM) combined with a DIM19. The depth resolution of such measurements is determined by the signal-to-noise ratio (SNR) of the measurement, and the SNR is in principle limited by the SQL. In the advanced measurements using DIM, the intensity of the probe light, focused onto a tiny area of B10 13 m2, is tightly limited for a non-invasive measurement, and the limit of the SNR is becoming a critical issue.

In this work, we demonstrate an entanglement-enhanced microscope, consisting of a confocal-type differential interference contrast microscope equipped with an entangled photon source as a probe light source, with an SNR of 1.35 times better than that of the SQL. We use an entangled two-photon source with a high delity of 98%, resulting in a high two-photon interference visibility in the confocal microscope setup of 95.2%. An image of a glass plate sample, where a Q shape is carved in relief on the surface with a ultra-thin step of B17 nm, is obtained with better visibility than with a classical light source. The improvement of the SNR is 1.350.12, which is consistent with the theoretical prediction of 1.35. We also conrm that the bias phase dependence of the SNR completely agrees with the theory without any tting parameter. We believe this experimental demonstration is an important step towards entanglement-enhanced microscopy with ultimate sensitivity.

ResultsThe limit of differential interference contrast microscope. Our entanglement-enhanced microscope is based on a laser confocal microscope combined with a differential interference contrast microscope (LCMDIM)19,20. An LCMDIM can detect a very tiny difference between optical path lengths in a sample. The LCMDIM works on the principle of a polarization interferometer (Fig. 1a). In this example, the horizontal (H) and vertical (V) polarization components are directed to different optical paths by a Nomarski prism. At the sample, the two beams experience different phase shifts (DfH and DfV) depending on the local refractive index and the structure of the sample. After passing through the sample, the two beams are combined into one beam by another Nomarski prism. The difference in the phase shifts can be detected as a polarization rotation at the output, Df DfV DfH.

We obtain differential interference contrast images for a sample by scanning the relative position of the focused beams on the sample (Fig. 1ce). When two beams probe a homogeneous region, the output intensity is constant (Fig. 1c). At the boundary

of the two regions, the signal intensity increases or decreases, as the difference in the phase shift Df becomes non-zero (Fig. 1d).

The signal intensity returns to the original level after the boundary (Fig. 1e). The smallest detectable change in the phase shift is limited by the SNR, which is the ratio of the change in the signal intensity, C(f), and the uctuation of the uniform background level, DC, at a bias level of F0. As is discussed in detail later, it is known that the SNR is limited by so-called shot noise or the SQL, when classical light sources such as lasers or lamps are used. That is, for a limited number of input photons (N), the SNR is limited by

Sample

Photo detector

Photon number

discriminating detector

Phase shift: [p10]

Polarizer

Intensity

measurement

Half wave

plate

Correlation

measurement

Nomarski prism Lens

c d e

Above

Side

Detected count

Detected count

Detected count

Position Position Position

Figure 1 | LCMDIM and the entanglement-enhanced microscope.(a) Illustration of LCMDIM (b) Illustration of the entanglement-enhanced microscope. The red and blue lines indicate horizontally and vertically polarized light. (ce) The change in the signal while the sample is scanned.

N

p . This SNR limits the height resolution of the LCMDIM when used to observe elementary steps at the surface of ice crystals19 or the difference in refractive indexes inside a sample. Thus, improving the SNR beyond the SQL is a revolutionary advance in microscopy.

Entanglement-enhanced microscope. We propose to use multi-photon quantum interference to beat this standard quantum limit (Fig. 1b). Instead of a classical light, we use an entangled photon state ( jN; 0iHV j0; NiHV= 2

p ), so-called NOON state, which is a quantum superposition of the states N photons in the H polarization mode and N photons in the V polarization mode. The phase difference between these two states is NDf after passing through the sample, which is N times larger than the classical case (N 1). At the output, the result of the multi-

photon interference (the parity of the photon number in the output) is measured by a pair of photon number discriminating detectors21,22.

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As is well known from two-path interferometry with N-photon states, entanglement can increase the sensitivity of a phase measurement by a factor of

N

p . In the entanglement-enhanced microscope, we apply this effect to achieve an SNR that is

N

p

p

. The SNR is then given by

SNR 1 x k

2

r NVN

sinNF0j

j

times higher than that of the LCMDIM. If the average number of N-photon states that pass through the microscope during a given time interval is k, then the average number of detection events in the output is given by C(f) kP(f), where P(f) (1 VN cos(Nf NF0))/2 is the probability of detecting an odd (or

even) number of photons in a specic output polarization (see Methods section). For a small positive phase shift of Df 1=N,

the phase dependence of the signal is given by the slope of C(f) at the bias phase F0, limDf-0|C(Df) C(0)| |qC(f)/qf||f

0

p

1 VN cos NF0

Df 1

where x is the normalized overlap region between the two beams at the sample plane (see Methods section). By maximizing the function of cos(NF0), we can nd the maximum sensitivity (SNRmax) as follows:

SNRmax 1 x k

Df, and the SNR is given by the ratio of the slope and the

statistical noise of the detection. If the emission of the N-photon states is statistically independent, the statistical noise is given by DC j f0

kP0

p N

1

1 V2N

r Df 2

q

a

BBO

crystals Filter

Single mode fibre Multi mode fibre

Half wave

plate PBS

PP

a

b

Sample

Coupler

Calcite Lens

Single photon

counting modules

Coincidence

measurement

c

103

b

Single counts (s1 )

20 15 10

5 0

0 1 2 3 4 5 6

Phase (radian)

0 1 2 3 4 5 6

Phase (radian)

0.4

0.2

0.0

HH

HV

VH

VV

HH

d

VV

103

Coincidence counts

(per 5 s)

VH

4

2

0

HV

Figure 2 | Experimental setup for a two-photon entanglement-enhanced microscope. (a) BBO: beta barium borate; PP: phase plate; PBS: polarizing beam splitter. A 405-nm diode laser (line width o0.02 nm) was used for the pump beam. A sharp-cut lter with a cut-off wavelength below 715 nm and a band pass lter with 4 nm bandwidth were used. The beam displacement at the calcite crystal was 4 mm. PBS reects vertical polarization component and transmits horizontal polarization. The blue and red lines indicate the optical paths for the horizontally and vertically polarized beams. The inset shows an illustration of the sample with the trajectory of the two beams. (b) The absolute value of the reconstructed density matrix of the generated state measured before the microscope setup (after the output coupler) using quantum state tomography45. HV denotes for the state where one photon is horizontally polarized and the other photon is vertically polarized. (c) Single-photon interference fringes of a classical microscope (counts per second) after the dark counts of the detectors (100 cps) subtracted. (d) Two-photon interference fringes of an entanglement-enhanced microscope (5 cps). While varying the phase by rotating the phase plate (PP), we counted the detected events in the output. The classical fringe was measured by inserting a polarizer transmitting the diagonally polarized photons and counting single-photon detection events. Solid lines in c and d are by theoretical tting curve. Error bars are given by Poissonian statistics.

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at a bias phase of

cosNF0 1

1 V2N

j iHV

=VN: 3

For the ideal case of VN 1, the maximum sensitivity

SNRmax k

p N Df. As the SNR for a classical microscope

using kN photons SNRmax

kN

q

j iHV= 2

p is generated from two beta barium borate crystals23,24 and is then delivered to the microscope setup via a single-mode bre. The polarization-entangled state is then converted to a two-photon NOON state 2

j ia 0

0; 2

= 2

j ib 0

j ia 2

j ib

p Df, an entanglement-

enhanced microscope can improve the SNR by a factor of

N

compared with a classical microscope for the same photon number.

Experimental setup. We demonstrate an entanglement-enhanced microscope (Fig. 2a) using a two-photon NOON state (N 2).

First, a polarization-entangled state of photons 2; 0

p using a calcite crystal25 and focused by an objective lens. From the result of quantum state tomography (Fig. 2b), the delity of the state is 98% and the entanglement concurrence is 0.979, which ensures that the produced state is almost maximally entangled. The entangled photons pass through two neighbouring spots at the sample plane (Fig. 2a inset). Then, after passing through the collimating lens, the two paths are merged by a polarization beam splitter and the result of the two-photon interference is detected by a pair of single-

100

0

p

a b

(nm)

100

50

9060300 (nm)

150 100 50 0 (m)

c d

800 m

800 m

Maximum

Minimum

0 m

0 m

0 m

0 m

800 m 800 m

e f

Coincidence counts

(per 400 ms)

120 100

80 60 40

0 100 200 300 400

Position (m) Position (m)

Single counts

(per 50 ms)

140 120 100

80

0 100 200 300 400

g h

Frequency

100

80 60 40 20

0

Frequency

100

80 60 40 20

60 80 100 120

Counts Counts

0 80 100 120 140

Figure 3 | Experimental results. (a) Atomic force microscope (AFM) image of a glass plate sample (BK7) on whose surface a Q shape is carved in relief with an ultra-thin step using optical lithography. (b) The section of the AFM image of the sample, which is the area outlined in red in a. The height of the step is estimated to be 17.3 nm from this data. (c) The image of the sample using an entanglement-enhanced microscope where two-photon entangled state is used to illuminate the sample. (d) The image of the sample using single photons (a classical light source). (e,f) One-dimensional ne scan data for the area outlined in red in c and d for the same photon number of 920. The measurement was made at a bias phase of 0.41 (e) and 0.66 (f), where optimal bias phases are 0.38 and 0.67, respectively. Solid lines are theoretical tting curves (see text). (g,h) The histogram of the counts at 400 experimental points of the background level for e and f. Red curves are the Poisson distribution with the average of the counts at 400 points (83.3 and 110.9, respectively).

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photon counters and a coincidence counter. The sample is scanned by a motorized stage to obtain an image. The beam diameters at the sample plane and the distance between the centre of the beams are all 45 mm (x 0.046).

Figure 2c,d shows the single-photon and two-photon interference fringes using a classical light source and the NOON source, respectively. The fringe period of Fig. 2d is half that of Fig. 2c, which is a typical feature of NOON state interference. The visibilities of these fringes, Vc 97.10.4% (Fig. 2c) and

Vq 95.20.6% (Fig. 2d), suggests the high quality of the

classical and quantum interferences.

Obtained image beating the standard quantum limit. Figure 3 shows the main result of this experiment. We used a glass plate sample (BK7) on whose surface a Q shape is carved in relief with an ultra-thin step of B17 nm using optical lithography (Fig. 3a,b). Figure 3c,d shows the two-dimensional (2D) scan images of the sample using entangled photons and single photons, respectively. The step of the Q-shaped relief is clearly seen in Fig. 3c, whereas it is obscure in Fig. 3d. Note that for both images we set the bias phases to almost their optimum values given in equation 3 and the average total number of photons (N k)

contributed to these data are set to 920 per position assuming the unity detection efciency.

For more detailed analysis, the cross-section of the images (coincidence count rate per single count rate at each position) are shown in Fig. 3e,f. The solid lines are theoretical ts to the data where the height and position of the step and the background level are used as free parameters. For Fig. 3e, the signal (the height of the peak of the tting curve from the background level) is 20.211.13, and the noise (the standard deviation of each experimental counts from the background level of the tting curve) is 9.48 (Fig. 3g). Thus, the SNR is 2.130.12. Similarly, the signal, the noise and the SNR are 17.71.22, 11.25 (Fig. 3h) and 1.580.11 for Fig. 3f, where classical light source (single photons) is used. The improvement in SNR is thus1.350.12, which is consistent with the theoretical prediction of 1.35 (equation 2). The estimated height of the step was17.00.9 nm (quantum) and 16.61.1 nm (classical), and is consistent with the estimated value of 17.3 nm from the atomic force microscope image in Fig. 3b.

As shown in equation 1, the SNR depends on the bias phase. Finally, we test the theoretical prediction of the bias phase dependence given by equation 1 in actual experiments. Figure 4 shows the bias phase dependence of the SNR for the two-photon NOON source (Fig. 4a) and classical light source (Fig. 4b). The solid curve is the theoretical prediction calculated by equation (1), where we used the observed visibilities of the fringes in Fig 2c,d

for VN. The theoretical curves are in good agreement with the experimental results.

DiscussionNote that the entanglement-enhanced microscope we reported here is different from the entangled photon microscope theoretically proposed by Teich and Saleh26, which is the combination of two photon uorescence microscopy and the entangled photon source. In the proposal, the increase in two-photon absorption rate and the exibility in the selection of target regions in the specimen were predicted. The application of entangled photon sources for imaging also includes quantum lithography27, where the lateral resolution of the generated pattern is improved25,28, and ghost imaging29, where the spatial correlation of entangled photons is utilized. In this context, this work is the rst application of entanglement-enhanced optical phase measurement beyond the SQL for imaging including microscopy. Note also that the entanglement is indispensable to improve the SNR of the phase measurement beyond the SQL11,30. This situation is different from the improvement in the contrast of the ghost imaging using strong thermal light3137.

In conclusion, we proposed and demonstrated an entanglement-enhanced microscope, which is a confocal-type differential interference contrast microscope equipped with an entangled photon source as a probe light source, with an SNR 1.350.12 times better than the SQL. Imaging of a glass plate sample with an ultra-thin step of B17 nm under a low photon number condition shows the viability of the entanglement-enhanced microscope for light-sensitive samples. To test the performance of the entanglement-enhanced microscope, we used modest-efciency detectors, however, recently developed high-efciency number-resolving photon detectors would markedly improve detection efciency38,39. We believe this experimental demonstration is an important step towards entanglement-enhanced microscopy with ultimate sensitivity, using a higher NOON state, a squeezed state40,41 and other hybrid approaches42 or adaptive estimation schemes43,44.

Methods

SNR of an entanglement-enhanced microscope. To perform the parity measurement used by Gerry21 and Seshadreesan et al.22, it is required to count both of the events where even and odd number of photons are detected in the output. However, experimental implementations become much easier if it is sufcient for us to just count odd (or even) number photon-detection events. In addition, the distance between the two beams and the beam size may have effect on the SNR. Here we consider these technical effects on SNR and derive equation (1).

To derive equation (1), we consider that the two beams at the sample are separated at a distance of a along the x axis, and each set of N photons has

Gaussian distribution with a variance of s in the xy plane (Fig. 5). After passing through the sample, the two beams experience a phase shift (f) in the grey region (x40). The state, |C(x, y, f)i, after the sample is written as

Cx; y; f

j i

1

p cHx; y; f

j i eiNF cVx; y; f

j i

2

4

a b

103 103

SNR/Nk

60

40

20

0

0.0 1.0 2.0 3.0

Bias phase (radian)

SNR/Nk

60 40 20

0

0.0 1.0 2.0 3.0

Bias phase (radian)

where |cH(x,y,f)i and |cV(x,y,f)i represents the states of N photons in the

horizontal and vertical polarization modes, respectively, and F0 is a bias phase. We assume that the phase shift is described by a step function and the N photons are in the same spatial modes. These states are written as

cHx; y; f

j i R

1 1

R1 1

e iwxNf

p

f x a=2; y

p ^ay

H

x; y

1 N

0

j idxdy

N !

R

1 1

R1 1

e iwxNf

p

f x a=2; y

N; 0; x; y

j iHVdxdy;

Figure 4 | Dependence of the SNR on the bias phase. (a) Dependence of the SNR on the bias phase using an entanglement-enhanced microscope (N 2). (b) Dependence of the SNR on the bias phase using a classical

microscope. The SNR and its error shown by error bars were calculated from the experimental data similar to Fig. 3e,f taken for different bias phases. The total photon number Nk 1,150 for a and 1,299 for b. The solid

line shows the theoretical curve using equation (1).

jcVx; y; fi R

1 1

R1 1

e iwxNf

p

f x a=2; y

p ^ay

1 V x; y

N

0

j idxdy

N !

R

1 1

R1 1

e iwxNf

p

f x a=2; y

0; N; x; y

j iHVdxdy;

5

where |0i is a vacuum state, ^ay

H

x; y and ^ayVx; y are the creation operators in H

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y

0

x

[afii9846]

[afii9825]

Figure 5 | The schematic of the two probe beams. The beams are in V polarization (blue) and H polarization (red) on the sample, corresponding to Fig. 1ce. The grey shaded region has the phase change of f.

and V polarization modes at the position of (x,y), respectively, and w(x) is a step function that w(x) 0 for x r0 and w(x) 1 for x40. f(x a/2, y) and f(x a/2, y)

represent the N-photon probability densities in the horizontal and vertical polarization modes written as

f x; y

1

2ps2 e ; Z

Z1

1

1 f x; ydxdy 1: 6

After passing through the second calcite crystal, the two beams are displaced at a distance of a/2 for H polarization and a/2 for V polarization along the x axis,

resulting in two beams in the same spatial mode. The state can then be written as

C0x; y; f

j i

1

p cHx a=2; y; f

j i eiNF cVx a=2; y; f

j i

2 :

7

Here, we assume that the state is projected onto the state where odd number of photons are in the minus diagonal polarization mode at the output. The measurement operator in the basis of plus (P) and minus (M) diagonal polarization is therefore written as

^

R

1 1

R1 1

8 >

>

>

<

>

>

>

:

P1 n0

PN=2m1n; 2m 1; x; yj iPM PM n; 2m 1; x; yh j dxdy if N is even

R1 1

R1 1

8

P1 n0

PN 1=2m1n; 2m 1; x; yj iPM PM n; 2m 1; x; yh j dxdy if N is odd

where

n; m; x; yi

j PM

p ^ay

P

x; y

1 n

^

ayMx; y

m

n ! m !

p

0 n ! m !

1

n

1

p ^ay

H

1

2

x; y ^ay V

x; y

m 0

j i: 9

p ^ay

H

2

x; y ^ay V

x; y

The probability of odd number photon detection can then be written as

Pf C0x; y; f

^

12 1 cosNf NF01 xa 12 1 cosNF0xa

10

C0x;

y; f

R

R

1

where we denote the phase-independent term of

R

R

a=2

1 1 f x; ydxdy

1 a=2 f x; ydxdy as x(a) which is the overlap integral between H and V

polarized beams at the sample plane.

We now calculate the SNR of our microscope using the NOON state including the effect of the overlap between the two beams at the sample plane. If the emission of the N-photon states is statistically independent, the statistical noise at the bias phase DCf j f

0

p

is given by

DC0 k

p

kP0

r : 11 For a small positive phase shift of Df 1=N, the signal is

@Cf=@f

j jjf0 Df 1 xa

1

2 1 cosNF0

k2 N sinNF0

j j Df: 12 Considering the visibility of the interference fringe VN, the SNR is given by

SNR 1 x k2

r NVN

1 VN cos NF0

Df: 13 Thus one can conrm that counting odd (or even) number photon-detection events can also achieve the phase super sensitivity. Note also that the dependence of SNR on the size and the distance between the two beams is simply given by

(1 x). This means that it is reasonable to compare the SNR between an

entanglement-enhanced microscope and a classical microscope (N 1) for

the same x.

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Acknowledgements

We thank Dr. Shouichi Sakakihara for sample fabrication, Professor Hidekazu Tanaka

and Dr. Koichi Okada for atomic force microscope measurements. We also thank

Professor Mitsuo Takeda, Professor Yoko Miyamoto, Professor Gen Sazaki, Professor

Holger F. Hofmann and Professor Masamichi Yamanishi for helpful discussion. This

work was supported in part by FIRST of JSPS, Quantum Cybernetics of JSPS, a Grant-in-

Aid from JSPS, JST-CREST, Special Coordination Funds for Promoting Science and

Technology, Research Foundation for Opto-Science and Technology and the GCOE

programme.

Author contributions

Experiments, measurements and data analysis were performed by T.O. with assistance of

R.O. and S.T. The project was planned by S.T. and supervised by R.O. and S.T. The

manuscript was written by all the authors.

Additional information

Competing nancial interests: The authors declare no competing nancial interests. Reprints and permission information is available online at http://npg.nature.com/reprintsandpermissions/

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http://npg.nature.com/reprintsandpermissions/

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How to cite this article: Ono, T. et al. An entanglement-enhanced microscope.

Nat. Commun. 4:2426 doi: 10.1038/ncomms3426 (2013).

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