Survey of sandfly vectors of leishmaniasis in Marambaia Island, municipality of Mangaratiba, State of Rio de Janeiro, Brazil

Abstract

Introduction: The influx and efflux of military personnel in the possible endemic areas of leishmaniasis provided the impetus for research on the sandflies on Marambaia Island.

Methods: Sandflies were collected with light traps installed in the 3 ecotypes of 3 areas chosen for their particular landscape aspects.

Results: In 2009, were collected 32,006 specimens of sandflies belonging to 13 species. The species that showed highest density were Nyssomyia intermedia and Migoneimyia migonei.

Conclusions: N. intermedia and M. migonei are the principal vectors of American tegumentary leishmaniasis (ATL) in Brazil; thus, extension studies related to the eco-epidemiology of these species of Marambaia Island are essential. [PUBLICATION ABSTRACT]

Short Communications

Survey of sandfly vectors of leishmaniasis in Marambaia Island, municipality of Mangaratiba, State of Rio de Janeiro, Brazil

Shênia Patrícia Corrêa NovoI, Marcos Barbosa de SouzaI, Ciro Benigno VillanovaI, Jairo Caetano MeródioI, Antônio de Medeiros MeiraI

IDepartamento de Ciências Biológias, Escola Nacional de Saúde Pública Sérgio Arouca, Fundação Oswaldo Cruz, Rio de Janeiro, RJ

ABSTRACT

Introduction

The influx and efflux of military personnel in the possible endemic areas of leishmaniasis provided the impetus for research on the sandflies on Marambaia Island.

Methods

Sandflies were collected with light traps installed in the 3 ecotypes of 3 areas chosen for their particular landscape aspects.

Results

In 2009, were collected 32,006 specimens of sandflies belonging to 13 species. The species that showed highest density were Nyssomyia intermedia and Migoneimyia migonei.

Conclusions

N. intermedia and M. migonei are the principal vectors of American tegumentary leishmaniasis (ATL) in Brazil; thus, extension studies related to the eco-epidemiology of these species of Marambaia Island are essential.

Keywords: Leishmaniasis; Sandfly fauna; Marambaia Island

Several sandfly species have been reported to carry natural infections by Leishmania spp. In Brazil, American tegumentary leishmaniasis (ATL) deserves attention due to its considerable magnitude and high risk of deformities in humans 1 . In the State of Rio de Janeiro, several studies relating to ATL epidemiology have indicated Nyssomyia intermedia as the species with the highest vector potential to transmit ATL in domicile and peridomicile environments in the region, because of its density, anthropophily, and easy adaptation to anthropic environments 2,3 . More recently, Migoneimyia migonei has been documented as another species with high vector potential 4 . According to the Ministry of Health in 2007 1 , AVL (also known as kala-azar and barriga d'água) has been expanding to urban areas. Its main vectors in Brazil are the Lutzomyia longipalpis and Lutzomyia cruzi species, which has been found in natural infections in Corumbá and Jaciara in the State of Mato Grosso do Sul 5,6 .

The risk of parasite transmission depends on the existence of a large number of vector species in addition to the emergence of species that used to be restricted to the wild. The epidemiological importance of these insects is linked to species that are effectively associated with the anthropic environment.

An important factor in Marambaia Island is the influx and efflux of military personnel in the possible endemic areas of leishmaniasis.

The objective of the present study was to determine the number of sandfly species and identify the epidemiologically important species for the transmission of leishmaniasis in the Center for Military Training in Marambaia Island (CADIM).

Marambaia Island (23°04?51?S - 44°00?39?W 23°03?48?S - 43°33?96?W) consists of an ecological reserve that has very distinct topography and vegetation, with a beach, restinga, hillside woods, and marsh areas 7 . This location is occupied by a quilombola community (remains of a quilombo, i.e., descendants of slaves). A survey carried out by the National Institute for Colonization and Agrarian Reform (INCRA) in 2006 estimated the existence of approximately 262 quilombola families in the region 8 . In addition, the island is visited annually by hundreds of thousands of people coming from different leishmaniasis-endemic regions in Brazil.

The present work was conducted in 3 distinct ecotopes (intradomicile, peridomicile, and woods) in 3 regions of Marambaia Island in the municipality of Mangaratiba: Pescaria Velha, Praia do Sítio, and Praia Grande. These regions were selected according to their landscape, vegetation, and anthropic activity characteristics.

The capture methodology included the use of the suction light trap (Centers for Disease Control [CDC] Model) 9,10 . Collection with light traps began at 6:00p.m. and ended at 7:00a.m. the next morning, totaling 13h of collection per trap. The collected specimens were placed in plastic tubes containing 70% alcohol. They were duly labeled and sent to the laboratory, where they were prepared and placed between microscope slides and cover slips in Canada Balsam. The collected specimens were then identified according to Galati's taxonomic nomenclature, 2003 11 .

Throughout the 12 collection months in the study area, we performed monthly collections of sandflies using a CDC-type light trap. A total of 32,006 specimens from 13 species were captured: N. intermedia, Migoneimyia migonei, Pintomyia fischeri, Lutzomyia longipalpis, Evandromyia edwardsi, Pintomyia bianchigalatiae, Brumptomyia brumpti, Evandromyia cortellezii, Expapillata firmatoi, Micropygomyia capixaba, Micropygomyia quinquefer, Micropygomyia schreiberi, and Psathyromyia pelloni. The species N. intermedia and M. migonei were predominant, representing 80% and 18.7% of the captured sandflies, respectively (Table 1).

TABLE 1 - Sandflies populations captured during 2009 in Marambaia Island, Rio de Janeiro, Brazil

Species Male Female Total

n % n % n %

Nyssomyia intermedia 12,259 48.0 13,320 52.1 25,579 80.0

Migoneimyia migonei 3,389 56.6 2,598 43.4 5,987 18.7

Pintomyia fischeri 21 12.3 149 87.6 170 0.5

Lutzomyia longipalpis 73 67.6 35 32.4 108 0.3

Evandromyia edwardsi 13 21.0 49 79.3 62 0.2

Pintomyia bianchigalatiae 4 11.0 33 89.2 37 0.1

Brumptomyia brumpti 10 30.3 23 70.0 33 0.1

Evandromyia cortellezii 0 0.0 2 100.0 2 0.006

Micropygomyia firmatoi 0 0.0 1 100.0 1 0.003

Micropygomyia capixaba 1 9.1 10 90.9 11 0.03

Micropygomyia quinquefer 1 16.7 5 83.3 6 0.02

Micropygomyia schreiberi 1 100.0 0 0.0 1 0.003

Psathyromyia pelloni 9 100.0 0 0.0 9 0.03

Total 15,781 49.3 16,225 50.7 32,006 100.0

Among the species collected throughout 2009, 4 were of epidemiological importance to leishmaniasis transmission in Brazil: L. longipalpis, M. migonei, N. intermedia, and P. fischeri.

The CADIM is a location of national and international traffic of military personnel from several leishmaniasis-endemic regions. Additionally, the location is occupied by a community of quilombola descendants. These facts motivated our team to collect sandflies at this location and to conduct a survey of the sandfly fauna to detect leishmaniasis vectors, as the presence of humans possibly infected with the disease in contact with its vectors enables the presence, increase, or decrease of disease transmission dynamics. The study of sandfly ecology in Rio de Janeiro was initiated when tegumentary leishmaniasis (TL) cases occurred in the former capital of the Republic 12 . In the first studies of TL in Brazil, the presence of N. intermedia was already evident in modified environments. However, it was only after research conducted by Forattini and Santos 13 that a high density of this vector was verified. Following this study, the hypothesis of its participation as the main transmitter of Leishmania braziliensis appeared 14 .

Pitta-Pereira et al. 4 reported a natural infection in N. intermedia and M. migonei in studies of natural identification by L. (Viannia) braziliensis in specimens collected from different neighborhoods throughout Rio de Janeiro. These species are highly anthropophilic, and are potential ATL vectors in Brazil.

The main American visceral leishmaniasis (AVL) vector in Brazil, L. longipalpis, was appointed by epidemiological evidence as the vector of this protozoonosis in the municipality of Rio de Janeiro 15 In the present study, L. longipalpis was captured in relatively low densities compared with species such as N. intermedia, an ATL vector. However, it is worth noting that since it was captured in the study locations and in greater density in the peridomiciles, in addition to the presence of seropositive dogs on Marambaia Island, there is a high possibility of the development of this disease.

The present study resulted in a great number of captured N. intermedia. This species was collected in all locations and all studied ecotopes, illustrating the high the level of importance of sandfly fauna studies on Marambaia Island. Since this species is of the greatest epidemiological importance for ATL in Brazil, it is vital to expand these studies related to eco-epidemiology on Marambaia Island. A similar result was observed for the M. migonei species, considered by many investigators as a secondary ATL vector in Brazil. The fact that there were 2 autochthonous ATL cases in the region, together with the finding of a great number of species that are vectors of this protozoonosis, adds to the importance of this study.

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Received: June9, , 2011; Accepted: September30, , 2011

Address to: Dra. Shênia Patrícia Corrêa Novo. Rua Leopoldo Bulhões 1480, Manguinhos, 21031-210 Rio de Janeiro, RJ, Brasil. Phone: [phone]. e-mail: [email protected]

CONFLICT OF INTEREST The authors declare that there is no conflict of interest.

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