Content area
Full Text
Abstract
In Thailand, some breeders in pig farms have had osteoarthritis lesions for which M. hyosynoviae might be one of the causative agents. Therefore, the semi-nested polymerase chain reaction assay was developed for detection of Mycoplasma hyosynoviae in organs of pigs in Thailand using three oligonucleotide primers specific to 16S rRNA gene of M. hyosynoviae. The detection limit of purified DNA was 10 femtogram per reaction and of the simulated lung sample was 10^sup 3^ CFU per gram of sample. Preliminary study for tonsil-carrier state of M. hyosynoviae in slaughtered pigs revealed the presence of M. hyosynoviae in 5 out of 10 farms. Thus, the semi-nested PCR is a useful tool for presumptive screening of M. hyosynoviae presenting in pig herds.
Keywords: Mycoplasma hyosynoviae, pig, semi-nested PCR
...
(ProQuest: ... denotes non-US-ASCII text omitted.)
Introduction
Mycoplasma hyosynoviae ( M. hyosynoviae), a non-cell wall prokaryote, belongs to the class Mollicutes. Family Mycoplasmataceae is one of the porcine mycoplasma pathogens and appears world- wide (Kobisch and Friis, 1996). At present, arthritis caused by M. hyosynoviae becomes an increasing problem in many countries (Neilsen et al., 2001; Assuncao et al., 2005; Schultz et al., 2012). M. hyosynoviae is the host specific bacterium in pigs and commonly inhabits upper respiratory tract such as nasal cavity, pharynx and tonsil of convalescent and adult animals. Adult pigs are important reservoirs of infection to piglets by direct contact. Therefore, pigs may occasionally develop pneumonic lesion and arthritis (Hagedorn-Olsen et al., 1999). M. hyosynoviae is an arginine metabolizing mycoplasma, which can be cultivated in Hayflick's medium supplemented with mucin and arginine. Primary isolation from tissues of pig is often complicated because of overgrowth of M. hyorhinis as well as other bacteria (Friis et al, 1991). Therefore, it is difficult to detect M. hyosynoviae by cultivation.
Enzootic pneumonia and porcine respiratory disease complex remain the major problems in swine production in Thailand. M. hyopneumoniae has been isolated from infected pigs since 1986 (Saitanu et al., 1989). In addition, M. hyorhinis causing pneumonia, polyserositis and arthritis in fattening pigs was also reported (Thongkamkoon et al., 2008). Although certain breeders have had osteoarthritis lesions similar to M. hyosynoviae arthritis, there has been no report confirming the detection of this bacterium. However, M. hyosynoviae has...