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Keywords. Rosa, acute kidney injury, antioxidants, histology
Introduction. This study aimed to investigate the effects of Rosa canina L fruit extracts on histological damages, oxidative stress, and functional disturbances induced by bilateral renal ischemia and reperfusion.
Materials and Methods. Ischemia and reperfusion were induced on the kidneys of anesthetized male Sprague-Dawley rats. The rats in the reperfusion and Rosa canina groups were administered extract solvent and Rosa canina extract, respectively. In addition, in the sham group, surgery was done without ischemia. In the last 6 hours of the reperfusion period, urine sample were collected using metabolic cage and at the end of this period, blood samples were taken from the descending aorta. The kidney tissues were collected and subjected to microscopic study for histological damages, while oxidative stress was measured by determining malondialdehyde and ferric reducing/antioxidant power levels.
Results. The comparison between the reperfusion and sham groups indicated reductions in creatinine clearance, absolute excretion of potassium, urine osmilarity, and increase in absolute excretion of sodium in the reperfusion group. These changes were less pronounced with Rosa canina fruit extract. In addition, blood creatinine and urea concentrations which increased in the reperfusion group, were significantly lower in the Rosa canina group. In this group, the degree of histological damages and the level of malondialdehyde were lower than the reperfusion group, while ferric reducing/antioxidant power level was significantly higher. Conclusions. The findings of this study showed that Rosa canina fruit extract possesses protective effects against kidney function disturbances, oxidative stress, and histological damages.
INTRODUCTION
Rosa canina is a herb belonging to the Rosaceae family, the usage of which as a medicinal plant dates back to Hippocrates time. During the World War II, it came to be used for preventing scurvy since it was rich in vitamin C.1 In traditional folk medicine, petals, fruit, and leaves of this plant are applied to the treatment of various diseases such as nephritis, common cold, flu, coughing, bronchitis, eczema, itching, and biliary diseases.2'3
Studies on the photochemical compounds found in Rosa canina have shown that its fruits contain phenolic acids, proanthocyanidins, tannins, flavonoids, fatty acids, pectines, carotenoids, and fruit acids (ascorbic acid, malic acid, and citric acid).4"14 Moreover, it has been shown that the fruit extract of Rosa canina...