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ABSTRACT:
A simple, sensitive, and precise High performance liquid chromatographic method for the analysis of Naproxen and Pantoprazole has been developed and validated for the determination of compounds in commercial pharmaceutical products. The compounds were well separated on a BDS Hypersil C-18 reversed-phase column by use of a mobile phase consisting of Acetonitrile and Mixed Phosphate buffer (pH 6.92) in 45:55(V/V%) ratio, at a flow rate of 1.0 mL/min with detection wavelength at 290 nm. The linearity ranges were 20-120µg. The correlation coefficient was found to be 0.997 and 0.995 for Naproxen and Pantoprazole respectively. The recovery amount was in the range of 99.67-101.39%. The high recovery and low relative standard deviation confirms the suitability of the method for determination of Naproxen and Pantoprazole in capsule dosage forms.
KEYWORDS: Naproxen, Pantoprazole.
1. INTRODUCTION:
Naproxen (as shown in Fig: 1) is a propionic acid derivative related to the aryl acetic acid group of non-steroidal anti-inflammatory drugs. The chemical names for Naproxen and Naproxen Sodium are (S)-6-methoxy-a-methyl-2-naphthaleneacetic acid and (S)-6-methoxy-α-methyl-2-naphthaleneacetic acid, sodium salt, respectively1. Naproxen is commonly used for the reduction of pain, fever, inflammation, and stiffness caused by conditions including migraine, osteoarthritis, kidney stones, rheumatoid arthritis, psoriatic arthritis, gout, Ankylosing spondylitis, menstrual cramps, Tendinitis, and Bursitis2,3. It is also used for the treatment of primary dysmenorrhea. Naproxen works by inhibiting both the COX-1andCOX-2 enzymes.Pantoprazole (as shown in Fig:2) is 6-(Difluoromethoxy)-2-[(3,4-dimethoxypyridin-2-yl)methylsulfinyl]-1H-benzimidazole].
Pantoprazole,a proton pump inhibitor drug used for short-term treatment of erosion and ulceration of the oesophagus caused by gastro-oesophageal reflux disease.
Pantoprazole is a proton pump inhibitor (PPI) that suppresses the final step in gastric acid production by forming a covalent bond to two sites of the (H+,K+ )-ATPase enzyme system at the secretory surface of the gastric parietal cell4,5. This effect is dose- related and leads to inhibition of both basal and stimulated gastric acid secretion irrespective of the stimulus.
Few analytical methods were found for this combination. Hence the present work is focused upon new method development and estimation of Naproxen and Pantoprazole.
2. MATERIAL AND METHODS:
2.1. Chemicals:
Naproxen was given as a gift by GlaxoSmithKline, England and Pantoprazole sodium was obtained from Dr. Reddy's, Hyderabad, India. All solvents used were of HPLC -grade and GR grade. Acetonitrile was purchased from...