Abstract/Details

In Situ Chondrocyte Mechanics and Mechanobiology

Madden, Ryan Matthew Jerome.   University of Calgary (Canada) ProQuest Dissertations Publishing,  2013. MR96866.

Abstract (summary)

Chondrocyte metabolism is stimulated by mechanical loading and is associated with structural changes in the cartilage extracellular matrix (ECM). Calcium signaling is an initial step in the biological response of cells to mechanical loading. The purpose of this work was to measure local ECM and chondrocyte deformations for a range of tissue strains and to relate the measured deformations to chondrocyte calcium signaling in intact cartilage attached to its native bone. We observed that:

1. Chondrocytes are protected from excessive deformation when cartilage is subjected to extreme compressive strains, likely due to the local extra- and peri-cellular matrices;

2. Chondrocyte calcium signaling is strongly correlated to compressive loading magnitude and the local ECM strains within the tissue; and,

3. Chondrocyte mechanobiology varies topographically within a joint.

These results provide new insights into the relationship between compressive mechanical loading, the resulting tissue and cell deformations, and the calcium signaling response of the chondrocytes.

Indexing (details)


Subject
Cellular biology;
Biomedical engineering
Classification
0379: Cellular biology
0541: Biomedical engineering
Identifier / keyword
Biological sciences; Applied sciences; Bone; Cartilage; Cartilage extracellular matrix; Chondrocyte deformations; Mechanobiology
Title
In Situ Chondrocyte Mechanics and Mechanobiology
Author
Madden, Ryan Matthew Jerome
Number of pages
120
Degree date
2013
School code
0026
Source
MAI 52/04M(E), Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
978-0-494-96866-6
Advisor
Herzog, Walter
University/institution
University of Calgary (Canada)
Department
Engineering
University location
Canada -- Alberta, CA
Degree
M.Sc.
Source type
Dissertation or Thesis
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
MR96866
ProQuest document ID
1475268319
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
https://www.proquest.com/docview/1475268319