Abstract

Doc number: 57

Abstract: Rapidly adapting mechanically activated channels (RA) are expressed in primary afferent neurons and identified as Piezo2 ion channels. We made whole-cell voltage-clamp recordings from cultured dorsal root ganglion (DRG) neurons to study RA channel regulation. RA currents showed gradual increases in current amplitude (current "run-up") after establishing whole-cell mode when 0.33 mM GTP or 0.33 mM GTPγS was included in the patch pipette internal solution. RA current run-up was also observed in HEK293 cells that heterologously expressed Piezo2 ion channels. No significant RA current run-up was observed in DRG neurons when GTP was omitted from the patch pipette internal solution, when GTP was replaced with 0.33 mM GDP, or when recordings were made under the perforated patch-clamp recording configuration. Our findings revealed a GTP-dependent up-regulation of the function of piezo2 ion channels in DRG neurons.

Details

Title
GTP-dependent run-up of Piezo2-type mechanically activated currents in rat dorsal root ganglion neurons
Author
Jia, Zhanfeng; Ikeda, Ryo; Ling, Jennifer; Gu, Jianguo G
Pages
57
Publication year
2013
Publication date
2013
Publisher
BioMed Central
e-ISSN
1756-6606
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1756-6606-6-57
ProQuest document ID
1491385634
Copyright
© 2013 Jia et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.