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Eur Food Res Technol (2014) 239:305311 DOI 10.1007/s00217-014-2223-y
ORIGINAL PAPER
Effect of ethanol fraction of burdock leaf on biolm formation and bacteria growth
Zaixiang Lou Ying Hong Yijun Liu Xinyi Song Lianzhong Ai Hongxin Wang Aiquan Jiao Yuxia Tang
Abstract The inhibition effects of burdock leaf ethanol fraction on the viability, biolm formation of food-related bacteria and the mechanism were rst investigated. The results showed that burdock leaf ethanol fraction signicantly inhibited the formation of biolm by Staphylococcus aureus and Listeria monocytogenes. The lowest concentration of the fraction that showed 100 % inhibition on the formation of L. monocytogenes biolm was 2.50 mg/ml, which was equal to the minimum inhibitory concentration (MIC) against bacterial growth. As for S. aureus, the lowest concentration of burdock leaf fraction that completely inhibited (100 %) the formation of biolm was found to be 1.25 mg/ ml, which was lower than MIC of the fraction (2.5 mg/ml). Thus, the biolm inhibition effect of burdock leaf fraction against S. aureus was not completely due to the inhibition on bacteria growth. Then, the effects of burdock leaf fraction on quorum sensing signals were evaluated through GCMS/MS analysis. The results indicated that burdock leaf fraction interfered with quorum sensing system and changed the composition of signaling molecules, thereby
Received: 16 March 2013 / Revised: 7 February 2014 / Accepted: 18 February 2014 / Published online: 13 April 2014 Springer-Verlag Berlin Heidelberg 2014
affecting the function of the quorum sensing system, signicantly inhibiting the formation of biolm. Then, the chemical composition of burdock leaf fraction was analyzed by ultra performance liquid chromatography (UPLC)MS/MS and eight active compounds were identied. The burdock leaf fraction based on its interfering with quorum sensing systems and the signicant inhibition on the formation of biolm could be highly useful in control of biolms.
Keywords Burdock leaf fraction Biolm inhibition
Bacteria growth inhibition Quorum sensing signals
Introduction
Biolm is a serious source of pollution in food industry sectors including fresh produce, dairy processing, poultry processing and red meat processing [1]. When the contamination of food occurs, the source of the problem is often biolm. A great deal of evidence indicates that biolm mode of life leads to growing resistance to antibacterial agents [2]. Compared to planktonic cells, biolms are more...