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Abstract
A Gram-stain positive, non-motile, rod-shaped bacterium, designated strain 1111S-42^sup T^, was isolated from the East Siberian Sea. The organism was found to grow at 4-30 °C, pH 7.0-8.5 and in 0-8 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 7.5 and in 1 % NaCl. Based on 16S rRNA gene sequence similarity studies, strain 1111S-42^sup T^ was found to belong to the genus Sporosarcina and to be most closely related to Sporosarcina contaminans CCUG53915^sup T^ (97.3 %) and Sporosarcina soli I80^sup T^ (97.2 %). The main polar lipids were found to include diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant menaquinone was identified as MK-7. The major cellular fatty acids were identified as anteiso-C^sub 15:0^ (34.4 %), iso-C^sub 15:0^ (29.8 %) and anteiso-C^sub 17:0^ (22.4 %). The DNA G+C content of strain 1111S-42^sup T^ was determined to be 39 mol %. The values of DNA-DNA relatedness between the strain 1111S-42^sup T^ and related type strains of the genus Sporosarcina were less than 30 %. Based on the phylogenetic analysis, along with extensive physiological and chemotaxonomic testing, we conclude that the bacterium represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siberiensis sp. nov. is proposed. The type strain is strain 1111S-42^sup T^ (=CGMCC 1.12516^sup T^ = LMG 27494^sup T^).[PUBLICATION ABSTRACT]