Abstract

Doc number: 19

Abstract

Background: Dioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal plants for diosgenin extraction, a rapid method to compare the contents of diosgenin in various plants is crucial.

Results: An ultra-performance liquid chromatography (UPLC) coupled with diode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for identification and determination of diosgenin in various plants. A comprehensive validation of the developed method was conducted. Twenty-four batches of plant samples from four Dioscorea species, one Smilax species and two Heterosmilax species were analyzed by using the developed method.

The present method presented good sensitivity, precision and accuracy. Diosgenin was found in three Dioscorea species and one Heterosmilax species, namely D. zingiberensis , D. septemloba , D. collettii and H. yunnanensis .

Conclusion: The method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting.

Details

Title
Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS
Author
Yi, Tao; Fan, Lan-Lan; Chen, Hong-Li; Zhu, Guo-Yuan; Suen, Hau-Man; Tang, Yi-Na; Zhu, Lin; Chu, Chu; Zhao, Zhong-Zhen; Chen, Hu-Biao
Pages
19
Publication year
2014
Publication date
2014
Publisher
BioMed Central
ISSN
14712237
e-ISSN
14712091
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1471-2091-15-19
ProQuest document ID
1553232441
Copyright
© 2014 Yi et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.