Abstract

Doc number: 45

Abstract

Background: Cell culture conditions can greatly influence the results of nanoparticle (NP) uptake assays. In this study, 10 nm gold nanoparticles (AuNPs) and RAW 264.7 macrophages were used as a model system, while instrumental neutron activation analysis (NAA) was used as the elemental analysis technique to determine AuNP levels produced by the various culturing conditions. Static plate-based and insert-based culture conditions were compared with a dynamic suspension culture to evaluate the conditions' effect on the rate and extent of AuNP uptake.

Results: The results indicate that a dynamic culturing condition allows for the greatest NP uptake (approximately 3-5 times over the adherent conditions), whereas the plate-based assays have the initial highest rate of NP incorporation.

Conclusions: These data highlight the importance of judiciously choosing the assay conditions prior to evaluating NP uptake.

Details

Title
Evaluating the effect of assay preparation on the uptake of gold nanoparticles by RAW264.7 cells
Author
Bancos, Simona; Tyner, Katherine M
Publication year
2014
Publication date
2014
Publisher
BioMed Central
e-ISSN
14773155
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s12951-014-0045-5
ProQuest document ID
1629780877
Copyright
© 2014 Bancos and Tyner; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.