Abstract

Doc number: 287

Abstract

Background: Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we identified the viral genes and their corresponding mRNA transcripts.

Results: The RNAs of P1, synthesized in porcine kidney cells, were examined with northern blotting and PCR analyses.

Eight virus-specific RNAs were detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, and 5) are encoded by the viral (-) strand and four (ORFs 3, 6, 7, and 8) are encoded by the viral (+) strand. All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length, except that encoded by ORF1 (113 amino acids).

Conclusions: We show a very complex viral transcription pattern in P1-infected cells.

Details

Title
Transcriptional analysis of porcine circovirus-like virus P1
Author
Wen, Li-bin; Wang, Feng-zhi; He, Kong-wang; Li, Bin; Wang, Xiao-min; Guo, Rong-li; Xie, Jian-ping
Publication year
2014
Publication date
2014
Publisher
BioMed Central
e-ISSN
17466148
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s12917-014-0287-3
ProQuest document ID
1635648011
Copyright
© 2014 Wen et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.