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Antonie van Leeuwenhoek (2015) 107:533538 DOI 10.1007/s10482-014-0349-3
ORIGINAL ARTICLE
Chryseobacterium rhizoplanae sp. nov., isolated from the rhizoplane environment
Peter Kampfer John A. McInroy
Stefanie P. Glaeser
Received: 5 November 2014 / Accepted: 2 December 2014 / Published online: 17 December 2014 Springer International Publishing Switzerland 2014
Abstract A slightly yellow pigmented strain (JM-534T) isolated from the rhizoplane of a eld-grown Zea mays plant was investigated using a polyphasic approach for its taxonomic allocation. Cells of the isolate were observed to be rod-shaped and to stain Gram-negative. Comparative 16S rRNA gene sequence analysis showed that the isolate had the highest sequence similarities to Chryseobacterium lactis(98.9 %), Chryseobacterium joostei and Chryseobacterium indologenes (both 98.7 %), and Chryseobacterium viscerum (98.6 %). Sequence similarities to all other Chryseobacterium species were 98.5 % or below. The fatty acid analysis of the strain resulted in a Chryseobacterium typical pattern consisting mainly of the fatty acids C15:0 iso, C15:0 iso 2-OH, C17:1 iso x9c,
and C17:0 iso 3-OH. DNADNA hybridizations with the type strains of C. lactis, C. joostei, C. viscerum and C.
indologenes resulted in values below 70 %. Genomic ngerprinting showed that the isolate was very different
to the type strains of these species. Differentiating biochemical and chemotaxonomic properties showed that the isolate JM-534T represents a novel species, for which the name Chryseobacterium rhizoplanae sp. nov. (type strain JM-534T = LMG 28481T = CCM 8544T = CIP 110828T) is proposed.
Keywords Chryseobacterium Rhizoplanae
Taxonomy
The genus Chryseobacterium described by Vandamme et al. (1994) now harbours a large number of species, some of which have been isolated from plant material including the rhizosphere/rhizoplane environment. Chryseobacterium formosense was isolated from the rhizosphere of Lactuca sativa L. (garden lettuce) in Taiwan (Young et al. 2005), Chryseobacterium soldanellicola and Chryseobacterium taeanense from roots of sand-dune plants (Park et al. 2006), Chryseobacterium luteum from the phyllosphere of grasses (Behrendt et al. 2007), Chryseobacterium gregarium from decaying plant material (Behrendt et al. 2008), Chryseobacterium elymi, Chryseobacterium hagamense, Chryseobacterium lathyri, and Chryseobacterium rhizosphaerae from the rhizo-sphere of coastal sand dune plants (Cho et al. 2010), Chryseobacterium ginsengisoli from the rhizosphere of ginseng (Nguyen et al. 2013), Chryseobacterium kwangjuense from pepper (Capsicum annuum L.)
Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s10482-014-0349-3
Web End =10.1007/s10482-014-0349-3 )...