Cascade N-Alkylation/Hemiacetalization for Facile Construction of the Spiroketal Skeleton of Acortatarin Alkaloids with Therapeutic Potentiality in Diabetic Nephropathy

Abstract

The concise building of the spiroketal core of acortatarin-type alkaloids as potential therapeutic agents in diabetic nephropathy was established in four steps, through a tandem N-alkylation/hemiacetalization between pyrrole units and the corresponding halo alcohols generated by convenient halomethylation of chiral lactones from natural aldoses.

The Acortatarin alkaloids exhibit a promising perspective against diabetic nephropathy and other ROS-linked diseases. Facile construction of the dioxaspirocycle motif was realized through tandem N-alkylation/hemiacetalization between pyrrole units and the corresponding halo alcohols, which were derived in turn from convenient halomethylation of chiral lactones in natural carbohydrates pool.[Figure not available: see fulltext.]

Nat. Prod. Bioprospect. (2015) 5:3745 DOI 10.1007/s13659-014-0049-8

ORIGINAL ARTICLE

Cascade N-Alkylation/Hemiacetalization for Facile Construction of the Spiroketal Skeleton of Acortatarin Alkaloids with Therapeutic Potentiality in Diabetic Nephropathy

Pei Cao Zhen-Jie Li Wen-Wu Sun

Shashwat Malhotra Yuan-Liang Ma

Bin Wu Virinder S. Parmar

Received: 3 November 2014 / Accepted: 27 November 2014 / Published online: 16 December 2014 The Author(s) 2014. This article is published with open access at Springerlink.com

Abstract The concise building of the spiroketal core of acortatarin-type alkaloids as potential therapeutic agents in diabetic nephropathy was established in four steps, through a tandem N-alkylation/hemiacetalization between pyrrole units and the corresponding halo alcohols generated by convenient halomethylation of chiral lactones from natural aldoses.

Keywords Acortatarin alkaloids Diabetic nephropathy N-alkylation/

hemiacetalization Halomethylation Chiral lactones

1 Introduction

Acortatarins A and B along with their structurally related analogues were independently isolated by three groups in 2010, from different natural sources used as traditional folk medicinal substances against a variety of ailments, including central nervous system disorders [1], prostatitis

[2] and rheumatics [3]. These compounds with the unique pyrrole-fused morpholine spiroketal architecture, possess a small group of scaffolds containing 2,5-bis-substituted pyrrole unit. Hou and Cheng demonstrated that acortatarins A and B have signicant antioxidant activity in a renal cell model for high-glucose-stimulated production of reactive oxygen species (ROS). Their results will provide a new template for further therapeutic intervention in diabetic nephropathy (DN) and other ROS-linked diseases [1]. Though they display promising and wide ranging biological properties, yet the strenuous extraction and purication procedures with very less yields of these special secondary metabolites from natural sources restrict their further biological investigation. Thus, considerable synthetic efforts have evoked to develop novel strategies for acortatarin analogues Fig 1.

Sudhakar [4] disclosed the rst total syntheses of acortatarins A and B followed by structural revisions of absolute congurations on both. Subsequently, Brimble [5] and Aponick [6] also nished the synthesis of acortatarin A. The wide-spread utility of these synthetic methodologies for acortatarins is limited because of tedious multi-steps (13 or more), low overall yields and disappointing or even undesired diastereoselectivity for spiroketalization. Compared superior to these methods, Tan et al. [7] developed an elegant, stereocontrolled syntheses of acortatarins A and B,

Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s13659-014-0049-8

Web End =10.1007/s13659-014-0049-8 ) contains supplementary material, which is available to authorized users.

P. Cao Z.-J. Li W.-W. Sun Y.-L. Ma B. Wu (&)

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Peoples Republic of China e-mail: [email protected]

W.-W. SunUniversity of Chinese Academy of Sciences, Beijing 100049, Peoples Republic of China

S. Malhotra V. S. Parmar (&)

Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi 110007, Indiae-mail: [email protected]

S. MalhotraInstitute of Chemistry and Biochemistry, Freie Universitat Berlin, Takustrasse 3, 14195 Berlin, Germany

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38 P. Cao et al.

Fig. 1 Revised structures of acortatarins A and B

however starting from expensive D-thymidine, using stoichiometric amounts of toxic mercury salt in the key step for acortatarin A. Recently, Kuwahara and coworkers [8] reported their expeditious route for acortatarin A derived from elaborately protected chiral olen, nevertheless, associated with higher cost and harsher deprotection/ cyclization steps. This particular architecture which appears simple comprises a signicant and attractive synthetic challenge indeed. Herein, we report our efforts towards facile building of the spiroketal core by extending the application of halo alcohols, which were generated by convenient halomethylation of chiral lactones from natural aldoses. An interesting tandem N-alkylation/hemiacetalization with pyrrole units was established to provide an alternative route for the target compounds.

2 Results and Discussion

The synthetic entries mentioned above are mainly based on SN2 reaction between the cyclic or acyclic ketose-type derivatives (epoxides [4], bromides [6, 8] or iodides [7]) and the pyrrole fragments, only with one example employing Maillard-type condensation [5]. Based upon the proposed biogenesis of acortatarins [2], we envisaged the chemoselective Amadori rearrangement to get the furanoid amino sugars, but our attempts did not give acceptable preparative yields (see supporting information). The work of Morin [9] and Wadouachi [10] made us recognize that the iodomethyl group acts as an interchanging hydroxymethyl or aminomethyl synthetic equivalent (Scheme 1). At the meanwhile, we noticed that the regioselective protection of fructofuranosyl acceptors directly from D-

fructose needs time-consuming long process [11]. Arajo [12] described an efcient approach for the furanoside originating from arabinose, taking advantage of the xed stereochemistry of the arabinolactone, which was easily prepared by the oxidation of the anomeric position with bromine in water. The one-carbon elongation of aldoses to ketoses in these cases used lithium reagent for the key homologation step. Enlightened by Arajos successful access to furanoside [12], an alternative retrospective route was outlined to obtain the key intermediates 3a and 3b from the readily available 2-deoxy-D-ribose (4a) [13] and D-arabinose (4b) [14], respectively. Similar to the protocol of Tan [7], the subsequently direct SN2 coupling with pyrrole partner 5 [10] could bridge the two subunits (Scheme 2). We reasoned that this plan might offer additional strategic advantages in that all the requisite stereo-chemistry of the hydroxyl groups for 1 and 2 would be attained or kept along with the initial natural aldoses from the beginning to the end. The versatility of assembling other unnatural acortatarin-type alkaloids was to succeed in conjunction with the implementation of one-step haloalkylation on natural chirons.

With the goal of devising a concise synthesis of this uncommon core, the exploration was initiated with the preparation of lactones 7a and 7b by the oxidation of 4a and 4b buffered in an aqueous K2CO3 solution (Scheme 3)

[14], then subjected to one-pot protection without further purication to get TBDMS ethers 8a and 8b in approximately 75 % overall yield for both (Scheme 3) [15]. Previously employed iodomethylation [9] for the conversion of 8a to 9 was proved to be unfavorable in this case, because it was very difcult to separate the iodide 9 from starting material recovered by chromatography along with liable decomposition in chloroform (or CDCl3). The focus shifted to the more stable bromomethylating compounds prepared by BrCH2Li (in situ generation from CH2Br2 and n-BuLi in the presence of LiBr) [16]. Unfortunately, an uncharacterized side product was observed after purication with slight decomposition by treatment of 8a with these combinational reagents. We were gratied to really furnish 10a as inseparable anomeric isomers (about 1:1 ratio) after venturously removing the additive LiBr. The yield (31 %) was modest, and the major byproduct, dihydrofuranone 10a0 [17] was delivered in 36 % yield. All attempts to enhance the ratio by changing the solvent (THF, toluene, DME), the amount of BrCH2Li (1, 1.5, 2 equivalents), reaction temperature (-65 or -78 C), running-time (0.5, 1, 2 h), or work-up in acidic medium (quenching by saturated ammonium chloride solution or equivalent acetic acid) failed (Scheme 3).

Pleasingly, 10b could be obtained in 71 % yield from 8b. With bromides 10a and 10b in hand to advance the synthesis, we decided to test the outcome of SN2 coupling

O

(Ox)

NH4OH NH4OH

O

O

OH

XCH2Li

O

OH O

CH2OH

OH TsCl

OH O

CH2OTs

OH

CH2X

O OH

NH2

Scheme 1 Transformations among halomethyl, hydroxymethyl and aminomethyl groups in ketose

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Cascade N-Alkylation/Hemiacetalization for Facile Construction 39

OH

4b: D-arabinose

HO

O

O

PGO

PGO

O

OH

HO

O

OH

or

HO X

N

R

HO

OHC

X = Br or I

1 Acortatarin A

N

O

4a: 2-deoxy-D-ribose

O OH

OH OH

3a: R = H 3b: R = OPG

+

HO

O

HO

R O

H

NH N

H

R

OH OHC 2

Acortatarin B

PG = Silyl or THP ethers

Scheme 2 Retrosynthetic route through key halomethylation of lactones from natural carbohydrates

O

R2

R = CHO or CH2OPG

5 6

O

HO

O

TBDMSO OH

O

OH

I

H

O

HO

TBDMSO

OH

OTBDMS

TBDMSO N

4a

O OH

OH OH

9

O O

N

H H H

TBDMSO

11 X

O H

I

III

V

13

HO

R1O

TBDMSO OH

O

R

O

IV O

R

TBDMSO

Br

O

RO

O

+

O

HO

R1O

7a: R = H 7b: R = OH

II

8a: R1 = TBDMS, R2 = H8b: R1 = TBDMS, R2 = OTBDMS 8c: R1 = Bn, R2 = H8d: R1 = TIPS, R2 = H

10a: R = H 10a': R = TBDMS 10b: R = OTBDMS

I III or IV

11

V

OH

4b

O

TIPSO OH X

14a: X = Br 14b: X = I

12a: R = TBDMS 12b: R = TIPS

11

O

OH

O

RO N

H O

TIPSO

VI

RO

Scheme 3 Unsatisfactory experimental trials with modication.(I) K2CO3, H2O, Br2, 0 C to rt. (II) a TBDMSCl, imidazole, DMAP, DMF, 0 C to rt, 75 % over two steps for 4a to 8a through 7a; 4b to 8b through 7b; b 2,4,6-tris(benzyloxy)-1,3,5-triazine, TfOH, dioxane, 0 C to rt, 65 % over two steps for 4a to 8c through 7a; c TIPSOTf, lutidine, DMAP, DMF, 0 C to rt, 70 % over two steps for 4a to 8d

through 7a. (III) CH2I2, n-BuLi, toluene, -82 C, 70 % for 8d to 14b.(IV) CH2Br2, n-BuLi, THF, -78 C, 31 % for 8a to 10a; 36 % for 8a to 10a0; 71 % for 8b to 10b; 36 % for 8d to 14a. (V) NaH, THF, 0 C to rt, 40 % for 10a to 12a. (VI) Cs2CO3, MeCN, rt, 60 % for 14b to 12b

with bisformyl pyrrole 11. The three-step sequence [18] with assistance of microwave for the VilsmeierHaack formylation [19] proceeded well to afford 11 in 45 % yield (Scheme 4). To our delight, 12a (nearly 1/1 diastereoisomers) was generated directly in about 40 % yield using NaH [10] with unanticipated formation of semiacetal ring which was conrmed by 1D and 2D NMR. From the more bulky 10b, reaction did not afford 13 at room temperature.

Moreover, decomposition happened under reux condition (Scheme 3). The replacement of NaH/THF system by Cs2CO3/MeCN [6], NaOH/toluene/H2O [7] did not lead to any improvements because of the inhibition of lactol formation.

The detailed functional compatibility resulted in the improvement for preparation of benzyl ethers (Scheme 3). Some problems were associated with the conventional O-

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40 P. Cao et al.

I II III

6 15 16 11

Scheme 4 Synthesis of pyrrole-2,5-dicarbaldehyde. (I) CNCH2CO2Et, piperidine, EtOH, reux. (II) oxalyl chloride - DMF, MW, 0100 C.(III) 3 M NaOH, reux, 45 % over three steps for 6 to 11

O

O

OEt

O O

NH H

N H

N H

NH H

OEt

NC

H

O

NC

H

O

benzylation methods in our hands, such as low yield for normal or mildly basic Williamson conditions (NaH/BnBr [4] or Ag2O/BnBr [9] ), tedious purication steps for benzyl 2,2,2-trichloroacetimidate (BTCAI) methodology [12, 20]. Better result was achieved nally to get benzyl ether 8c through 2,4,6-tris(benzyloxy)-1,3,5-triazine (Tri-BOT) [21] catalyzed by TfOH in one-pot (65 % total yield without further optimization) (Scheme 3). All the glitches with other protecting protocols including acetalization, ketalization, disilyl (TIPDSCl), THP, MOM, allyl ethers were tried, among which, excellent regioselectivity was realized to afford (3S,5R)-5-O-allyl-3-hydroxy-D-ribonolactone with moderate yield, by 2,4,6-tris(allyloxy)-1,3,5-triazine (TriAOT) (see supporting information). Due to the epimerization of debenzylation accomplished by TiCl4 [4,

6], we still wanted to make progress towards the appropriate silyl groups whose deprotection was much milder. Sauve [15] showed that the b-elimination in electrophilic uorination of 2-deoxyribonolactone can be successfully mitigated by steric triisopropylsilyl (TIPS) bulk at the susceptible b-position. So TIPS-protected lactone 8d [15]

was introduced and used for bromomethylation [16]. This precursor was unproductive to completely inhibit b-elimination with the major unsaturated dihydrofuranone easily monitored by basic KMnO4 solution. The isolated yield of 14a (nearly 1/1 diastereoisomers) was frustrating again (about 36 %) (Scheme 3). The iodomethylation of 8d with CH2I2 and n-BuLi (even 1.2/1 equivalents) was very complicated in THF. Changing the solvent to toluene [9]

gave very slow conversion to 14b even after 3 h, as shown by the crude NMR of the reaction mixture (see supporting information). However, we noticed that the b-elimination becomes negligible at low temperature (-82 C). We increased the ratio of CH2I2 and n-BuLi gradually. Finally, about 70 % isolated yield for 14b (nearly 1/1 diastereoi-somers) was realized with a slight excess of CH2I2 and n-

BuLi (3/2.8 equivalents) after running the reaction for 3 h under -82 C (Scheme 3).

Till this point, the unexpected tandem N-alkylation and concomitant semiacetalization for the lactol ring formation was the main problem left. 1,5-Disubstituted 5-hydroxy-1,4-diketone 19 [22] was introduced to allow the construction of bis-substituted pyrrole 20 with aldehyde group masked as olen (Scheme 5). Both 18 and 19 were obtained as reported with moderate yields in two steps [22].

PaalKnorr condensation of diketones 19 with NH4OAc were carried out under a variety of buffered or acidic conditions (HOAc-NaOAc, p-TsOH, CSA, I2). Only polymeric products or decomposition resulted, probably because the acid-catalyzed decomposition of the pyrrolic products is competitive with their formation. Ultimately, after screening dioxane, DME, THF, MeCN, EtOH, MeOH and H2O as solvents, one mild neutral buffering system worked only in EtOH (NH4OAc/NaOAc = 3.5/1.2 equivalents) to afford pyrrole unit 20 quantitatively. The direct coupling between iodide 14b and 20 proved ineffective. Moreover, the unpredictability of oxidative cleavage of olens to aldehydes and the additive protection/deprotection for hydroxyl group in 20 put us in a dilemma. At this juncture, the reductive deoxygenation of lactol to benzopyran achieved by exposure to NaBH4 [23] or triethylsilane (Et3SiH) [24] in the presence of TFA attracted our attention. Thus we could continue and complete the expected total synthesis of acortatarin A as illustrated in Scheme 6.

N-alkylation of pyrrole 11 with iodide 14b could deliver hemiacetal 12b (Cs2CO3/MeCN [6], nearly 1/1 diastereoisomers, 60 % yield without further optimization). The reduction of lactol 12b to create morpholine motif 21 would not be problematic though the free aldehyde could not be kept. The oxidation to 22 and deprotection of silyl ether for acortatarin A and its epimer could be nished according to the known literature methods. To minimize the synthesis steps and maximize the overall efciency of our strategy, one alternative route was proposed to introduce the second aldehyde group by formylation of 24 at a later stage as depicted.

3 Conclusion

We hypothesized that the thermodynamic stability of the nal products as the driving force in the cascade N-alkylation and concomitant semiacetalization. The spontaneous spiro-hemiacetalization was induced by the deprotonation of hydroxyl group adhering to the anomeric carbon at the ring junction position, followed by sequential attack of the resulting oxygen anion aiming at neighboring aldehyde group. This crucial semiketal carbon serves as one incorporating middle hinge necessary for the simultaneous shutting of the crab-claw-shaped morpholine ring (Scheme 6).

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Cascade N-Alkylation/Hemiacetalization for Facile Construction 41

O

O

O

I II

Ph

OH

III

HO Ph

Ph

OH

Ph

O CN

O

N H

17

18 19 20

Scheme 5 Synthesis of bis-substituted pyrroles. (I) Et3N, ClCH2CN, dichloroethane, 0 C to rt, 75 %. (II) EtMgBr, Ti(O-i-Pr)4, diethyl ether, 0 C to rt, 70 %. (III) NH4OAc, NaOAc, EtOH, MW, 40 C, quantitative conversion

O

N

H H H

O

O O

O

OH

H

TIPSO OH O

TIPSO

TIPSO N

H O

N H

O

OH

O

I

TIPSO N

TIPSO

tandem N-alkylation hemiacetalization

oxidation

TIPSO

23

14b 12b

21

reductive deoxygenation

O

O

TIPSO N

reductive deoxygenation

TIPSO

24

O

O

O

O

deprotection

formylation

O

O

HO N

H O

TIPSO N

H O

TIPSO N

HO

HO

TIPSO

TIPSO

1 + epimer

22

Scheme 6 On-going synthetic perspectives of acortatarin A

It may supply one alternative route for the troublesome coupling of pyrrole units bearing sterically hindered THP or TBDMS protected hydroxyl groups [4].

For the importance of deoxyribonucleotide chemistry, 2-deoxy-D-ribose derivatives were found to be more advantageous. But the stepwise improved halomethylating condition is adaptable within wider scope. The present experiences were envisioned to allow the assembly of the target molecules efciently, including the construction of all the subunits in the near future. The key elements in this synthesis sequence include the optimized halomethylation for lactones and tandem nucleophilic substitution. The other important features lie in prompting the utility of natural sugars as the chiral pool inspired by the biogenetic hypothesis. Obviously, when combined with the unnatural formylated pyrrole analogues with para-substitutions (C-2/5) (Scheme 5), such a synthetic strategy is also readily performed for other novel acortatarins. The pursuit towards these goals is currently underway, and the results will be reported in due course.

4 Experiment Section

Melting points were measured on an SGW X-4 melting instrument and uncorrected. MS were recorded on a Waters

AutoSpec Premier P776 or API STAR Pulsar instrument. NMR spectra were recorded on a Bruker AM-400 or DRX-500 spectrometer and calibrated using residual solvent peaks as internal reference, for example CDCl3 solutions.

TLC analyses were performed on commercial glass plates bearing 0.25 mm layer of Merck Silica gel 60F254. Silica

gel (200300 mesh, Qingdao Marine Chemical Co. Ltd., Peoples Republic of China) was used for general column chromatography. Spots on TLC plates were detected under UV radiation and by heating after spraying with 10 % H2SO4 in EtOH or dilute solutions of KMnO4. Microwave irradiation reactions were carried out in a CEM Discover

SP system. Tetrahydrofuran (THF), diethyl ether and toluene were distilled over sodium/benzophenone. Other reagents were obtained from commercial sources and used without further purication unless otherwise stated.

4.1 2-Deoxy-3,5-di-O-(tert-butyldimethylsilyl)-

D-ribonolactone (8a)

To a solution of 2-deoxy-D-ribose 4a (1.0 g, 7.5 mmol) in 6 mL water, K2CO3 (1.2 g, 8.9 mmol) was added in portions. After the clear aqueous solution was cooled to 0 C, Br2 (1.3 g, 8.2 mmol) was added dropwise. The resulting orange solution was warmed to rt and stirred for 10 h. The reaction mixture was quenched and neutralized

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42 P. Cao et al.

by the addition of 88 % formic acid, the solvent from the aqueous fraction was evaporated to give a pale brown syrup 7a, which was heated at 50 C under reduced pressure for 1 h. EtOH (20 mL) was added, and the solvent was repeatedly evaporated after each addition of EtOH. Without further purication, the crude product 7a was dissolved in 20 mL of anhydrous DMF, to which imidazole (2.5 g, 37.3 mmol) and tert-butyldimethylsilyl chloride (4.5 g, 29.8 mmol) were added. The resulting solution was stirred at rt for 24 h and quenched by addition of water. The water layer was extracted with ethyl acetate (30 mL 9 3), and the organic layers were combined, washed with brine, and dried over anhydrous Na2SO4. The crude product was concentrated in vacuo. Flash chromatography afforded 8a as a white solid: mp 7273 C. Yield:2.03 g (75 % over two steps) [14, 15].

1H NMR (400 MHz, CDCl3) d 4.49 (dt, J = 6.8,2.0 Hz, 1H), 4.33 (dd, J = 4.7, 2.3 Hz, 1H), 3.80 (dd, J = 11.5, 3.2 Hz, 1H), 3.75 (dd, J = 11.5, 2.4 Hz, 1H),2.81 (dd, J = 17.6, 6.8 Hz, 1H), 2.38 (dd, J = 17.6,2.4 Hz, 1H), 0.88 (s, 18H), 0.08 (s, 6H), 0.07 (s, 3H), 0.05 (s, 3H). 13C NMR (100 MHz, CDCl3) d 175.98, 88.22,69.71, 62.53, 39.13, 25.87, 25.73, 18.31, 17.99, -4.69, -4.76, -5.45, -5.57.

ESI-MS m/z (rel int): (pos) 383 ([M ? Na]?, 100).

4.2 2,3,5-Tri-O-(tert-butyldimethylsilyl)-D-arabino-1,4-lactone (8b)

The synthesis of lactone repeated the known procedure [14]. The O-silylation followed the literature method [15]. 8b was afforded as colorless oil. Yield: 368 mg (75 % over two steps).

1H NMR (400 MHz, CDCl3) d 4.34 (dd, J = 15.6,7.1 Hz, 1H), 4.30 (d, J = 7.1 Hz, 1H), 4.06 (dt, J = 6.3,3.1 Hz, 1H), 3.90 (dd, J = 12.0, 2.9 Hz, 1H), 3.76 (dd, J = 12.0, 3.3 Hz, 1H), 0.920.88 (overlapping s, 27H),0.160.08 (overlapping s, 18H). 13C NMR (100 MHz,

CDCl3) d 173.38, 82.21, 76.35, 74.03, 60.46, 25.92, 25.87,25.81, 18.40, 18.33, 17.94, -4.11, -4.25, -4.61, -4.72, -5.21, -5.30.

ESI-MS m/z (rel int): (pos) 514 ([M ? Na]?, 100).

4.3 2,4,6-Tris(benzyloxy)-1,3,5-triazine (TriBOT)

The synthesis of this reagent followed the known procedure, but using 1.2 equivalents NaH instead of NaOH and1.1 equivalents BnOH in THF, respectively. White solid: mp 106108 C. Yield: 638 mg (80 %) [21].

1H NMR (500 MHz, CDCl3) d 7.457.32 (m, 15H), 5.45 (s, 6H). 13C NMR (100 MHz, CDCl3) d 173.12, 135.41, 128.69, 128.55, 128.42, 70.04.

4.4 3,5-Di-O-benzyl-D-ribonolactone (8c)

The synthesis of lactone repeated the known procedure [14]. The O-benzylation followed the literature method [21]. 8c was afforded as colorless oil. Yield: 101 mg (65 % over two steps) [20].

1H NMR (400 MHz, CDCl3) d 7.397.26 (m, 10H), 4.64 (dd, J = 4.8, 2.9 Hz, 1H), 4.52 (m, 4H), 4.28 (dt, J = 6.8,1.9 Hz, 1H), 3.65 (qd, J = 10.7, 3.1 Hz, 2H), 2.87 (dd, J = 18.1, 6.9 Hz, 1H), 2.58 (dd, J = 18.1, 2.2 Hz, 1H).

13C NMR (100 MHz, CDCl3) d 175.73, 137.43, 137.13, 128.72, 128.66, 128.22, 128.08, 127.88, 127.74, 84.14,76.19, 73.82, 71.31, 69.70, 35.86.

EI-MS m/z (rel int): 312 (M?), 221 (M?-91), 107, 91

(100).

4.5 2-Deoxy-3,5-di-O-(triisopropylsilyl)-D-

ribonolactone (8d)

The synthesis of lactone repeated the known procedure [14]. The O-silylation followed the literature method, however, with slight modication by combinational TIPSOTf, lutidine (instead of TBDMSCl, imidazole) and DMAP in DMF [15]. 8d was afforded as colorless oil. Yield: 1.0 g (75 % over two steps).

1H NMR (400 MHz, CDCl3) d 4.65 (d, J = 6.4 Hz, 1H), 4.40 (s, 1H), 3.92 (dd, J = 11.3, 2.9 Hz, 1H), 3.86 (dd, J = 11.6, 2.0 Hz, 1H), 2.88 (dd, J = 17.6, 6.5 Hz, 1H), 2.42 (d, J = 17.6 Hz, 1H), 1.04 (s, 42H). 13C NMR (100 MHz, CDCl3) d 175.97, 88.71, 69.93, 63.19, 39.54,17.82, 11.89, 11.91.

ESI-MS m/z (rel int): (pos) 467 ([M ? Na]?, 100).

4.6 1-Bromo-1,3-dideoxy-4,6-di-O-(tertbutyldimethylsilyl)-D-fructofuranose (10a)

Under nitrogen protection, to a mixed solution of lactone 8a (360 mg, 1.0 mmol) and dibromomethane (348 mg,2.0 mmol) in dry THF (10 mL) cooled to -78 C was added dropwise a solution of n-butyllithium in hexane(2.4 M solution, 0.6 mL, 1.5 mmol). The solution was maintained at -78 C for 1 h then quenched with a saturated solution of ammonium chloride in water (about 4 mL). The organic layer was decanted, and the aqueous layer was extracted with diethyl ether (10 mL 9 2), the combined organic layers were dried over anhydrous Na2SO4 and the solvents evaporated under reduced pressure. Flash chromatography gave bromo alcohol 10a as pale yellow oil [9, 16]. Yield: 141 mg (31 %).

Isomer A (54 %) 1H NMR (400 MHz, CD3COCD3) d4.90 (br.s, 1H, OH), 4.41 (dt, J = 6.7, 4.4 Hz, 1H), 4.05 (q, J = 4.4 Hz, 1H), 3.683.66 (m, 2H), 3.49 (d, J = 2.1 Hz, 2H), 2.49 (dd, J = 13.3, 6.9 Hz, 1H), 1.99 (dd, J = 13.3,

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Cascade N-Alkylation/Hemiacetalization for Facile Construction 43

4.4 Hz, 1H), 0.91 (s, 18H), 0.120.08 (overlapping s, 12H).

13C NMR (100 MHz, CD3COCD3) d 104.94, 87.34, 73.57,63.42, 44.84, 38.51, 26.30, 26.12, 18.89, 18.45, -4.58, -4.67, -5.22.

Isomer B (46 %) 1H NMR (400 MHz, CD3COCD3) d5.10 (br.s, 1H, OH), 4.50 (dt, J = 5.8, 4.8 Hz, 1H), 3.87 (dd, J = 9.2, 4.8 Hz, 1H), 3.683.66 (m, 2H), 3.65 (d, J = 1.7 Hz, 1H), 3.56 (d, J = 10.3 Hz, 1H), 2.25 (qd, J = 13.3, 5.5 Hz, 2H), 0.91 (s, 18H), 0.120.08 (overlapping s, 12H). 13C NMR (100 MHz, CD3COCD3) d 105.58,88.67, 73.79, 64.55, 44.44, 39.66, 26.30, 26.12, 18.89,18.45, -4.58, -4.67, -5.22.

ESI-MS m/z (rel int): (pos) 477 ([M ? Na]?).

4.7 (5S)-5-(tert-Butyldimethylsilanyloxymethyl)-5H-furan-2-one (10a0)

The modied procedure for bromomethylation was as same as that of 10a [9, 16], the side-product dihydrofuranone 10a0 was afforded as pale yellow oil. Yield: 82 mg (36 %) [17].

1H NMR (400 MHz, CDCl3) d 7.49 (dd, J = 5.7,1.0 Hz, 1H), 6.16 (dd, J = 5.7, 1.6 Hz, 1H), 5.05 (t, J = 4.7 Hz, 1H), 3.92 (dd, J = 10.8, 4.4 Hz, 1H), 3.80 (dd, J = 10.8, 5.3 Hz, 1H), 0.87 (s, 9H), 0.06 (s, 6H). 13C

NMR (100 MHz, CDCl3) d 173.15, 154.45, 122.64, 83.47,63.03, 25.99, 22.83, 14.27, -5.38.

4.8 1-Bromo-1-deoxy-3,4,6-tri-O-(tertbutyldimethylsilyl)-D-fructofuranose (10b)

The modied procedure for bromomethylation was as same as that of 10a [9, 16], 10b was afforded as colorless oil. Yield: 416 mg (71 %).

Isomer A (57 %) 1H NMR (500 MHz, CD3COCD3) d4.49 (d, J = 1.9 Hz, 1H), 4.274.26 (m, 1H), 4.224.21 (m, 1H), 3.833.79 (m, 1H), 3.71 (d, J = 6.0 Hz, 1H), 3.69 (d, J = 10.5 Hz, 1H), 3.66 (d, J = 6.0 Hz, 1H), 3.58 (d, J = 10.5 Hz, 1H), 0.980.82 (overlapping s, 27H),0.240.09 (overlapping s, 18H). 13C NMR (125 MHz,

CD3COCD3) d 105.78, 87.11, 79.60, 79.51, 64.51, 36.41,26.37, 26.33, 26.28, 26.12, 26.08, 18.88, 18.77, 18.54, 18.38, -3.80, -4.22, -4.30, -4.34, -4.45, -4.52, -4.68, -5.10, -5.17.

Isomer B (43 %) 1H NMR (500 MHz, CD3COCD3) d4.67 (d, J = 1.0 Hz, 1H), 4.244.23 (m, 1H), 4.104.09 (m, 1H), 4.084.07 (m, 1H), 3.71 (d, J = 6.0 Hz, 1H), 3.66 (d, J = 6.0 Hz, 1H), 3.54 (dd, J = 10.3, 1.1 Hz, 1H), 3.47 (d, J = 10.3 Hz, 1H), 0.980.82 (overlapping s, 27H),0.240.09 (overlapping s, 18H). 13C NMR (125 MHz,

CD3COCD3) d 106.75, 87.92, 82.58, 79.64, 64.38, 34.88,26.37, 26.33, 26.28, 26.12, 26.08, 18.88, 18.77, 18.54, 18.38, -3.80, -4.22, -4.30, -4.34, -4.45, -4.52, -4.68, -5.10, -5.17.

ESI-MS m/z (rel int): (pos) 609 ([M ? Na]?, 100).

5 1H-Pyrrole-2,5-dicarbaldehyde (11)

A solution of pyrrole-2-aldehyde 6 (0.48 g, 5 mmol), ethyl cyanoacetate (0.68 g, 6 mmol) and piperidine (0.05 mL) in ethanol (5 mL) was stirred at rt for 1 h then heated to reux for 2 h under the protection of nitrogen. The reactants were cooled to rt, the orange solids precipitated were ltrated and washed by petroleum ether, after removal of the volatiles in vacuo, 15 was obtained in quantitative yield. 3 mL DMF was cooled below 5 C in a round-bottom ask equipped with a stir bar, under protection of nitrogen, oxalyl chloride (0.76 g, 6 mmol) was added under vigorous stirring, the cooling bath was removed, 30 min later, the mixture was cooled to 0 C again. A solution of compound 15 in 2 mL of DMF was added and the resulting mixture was allowed to stir in an ice bath for 45 min and at rt for 2 h. The ask was then quickly tted with a short condenser pipe protected by calcium chloride drying tube and heated by microwaves at 100 C for 12 min in a CEM-microwave reactor. After cooling to rt, a solution of saturated aqueous NaHCO3 was added carefully to adjust the pH to slightly basic, and the mixture was heated for 1530 min under reux. After cooling, the crude precipitate 16 was ltered off and added to a solution of 15 mL3 M NaOH, the mixture was reuxed for 2 h under nitrogen, cooled, neutralized with 6 M hydrochloride acid in an ice bath, the precipitate resulted was ltered. The aqueous phase was extracted by ethyl acetate (20 mL 9 3), the total organic level was dried over anhydrous Na2SO4, after removal of the solvents in vacuo, the solids ltrated previously and the organic phase were combined and subjected to ash column chromatography on silica gel to afford 11 as pale yellow solid: mp 120122 C. Yield:0.32 g (52 % over three steps) [18, 19].

1H NMR (400 MHz, CDCl3) d 10.49 (br.s, 1H). 9.79 (s,

2H), 7.02 (d, J = 2.2 Hz, 2H). 13C NMR (100 MHz,

CDCl3) d 181.62, 135.83, 119.69.ESI-MS m/z (rel int): (neg) 122.0 ([MH]-, 100).

5.1 (4S,5R)-10-Hydroxy-4-((tertbutyldimethylsilyl)oxy)-5-(((tertbutyldimethylsilyl)oxy)-methyl)-10,4,40,5-tetrahydro-3H-spiro[furan-2,30-pyrrolo[2,1-c] [1,4]

oxazine]-60-carbaldehyde (12a)

To a solution of 1H-Pyrrole-2,5-dicarbaldehyde 11 (15 mg,0.12 mmol) in dry THF (2 mL) under argon at 0 C, sodium hydride 60 % in oil (4 mg, 0.1 mmol) was added. After 45 min at rt, the orange solution was cooled to 0 C, and a solution of bromo alcohol 10a (68 mg, 0.15 mmol)

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44 P. Cao et al.

in dry THF (2 mL) was cautiously added. After 4 h at rt, the mixture was quenched with a saturated solution of NH4Cl, extracted with CH2Cl2 (4 mL 9 2). The combined organic phase was washed with brine, dried over Na2SO4, ltered and evaporated to dryness. After purication by ash chromatography on silica gel, the lactol 12a (nearly 1/1 diastereoisomers) was provided as pale yellow oil [10]. Yield: 22 mg (42 %).

Isomer A (54 %) 1H NMR (500 MHz, CD3COCD3) d11.23 (br.s, 1H), 9.55 (s, 1H), 6.94 (d, J = 2.6 Hz, 1H),6.43 (dd, J = 3.5, 2.3 Hz, 1H), 6.02 (s, 1H), 4.52 (td, J = 5.7, 4.0 Hz, 1H), 4.15 (d, J = 8.5 Hz, 1H), 3.99 (d, J = 8.7 Hz, 1H), 3.93 (m, 1H), 3.69 (dd, J = 5.4, 1.3 Hz, 2H), 2.582.55 (m, 1H), 2.212.16 (m, 1H), 0.920.88 (overlapping s, 18H), 0.160.08 (overlapping s, 12H). 13C

NMR (100 MHz, CD3COCD3) d 180.01, 137.15, 134.71, 120.34, 112.89, 111.08, 98.09, 88.48, 74.72, 73.15, 64.43,43.15, 26.28, 26.25, 26.13, 26.10, 18.90, 18.54, 18.47, -4.49, -4.57, -4.68, -4.69, -5.07, -5.17.

Isomer B (46 %) 1H NMR (400 MHz, CD3COCD3) d11.25 (br.s, 1H), 9.55 (s, 1H), 6,95 (d, J = 2.3 Hz, 1H),6.45 (dd, J = 3.7, 2.3 Hz, 1H), 6.03 (s, 1H), 4.40 (dt, J = 8.1, 5.0 Hz, 1H), 4.10 (d, J = 8.5 Hz, 1H), 3.963.94 (m, 1H), 3.93 (m, 1H), 3.81 (dd, J = 11.5, 3.1 Hz, 1H),3.75 (dd, J = 11.5, 3.9 Hz, 1H), 2.622.59 (m, 1H), 2.262.22 (m, 1H), 0.920.88 (overlapping s, 18H), 0.160.08 (overlapping s, 12H). 13C NMR (100 MHz, CD3COCD3) d 180.01, 137.15, 134.71, 120.34, 112.43, 110.99, 98.17,87.38, 74.29, 71.99, 63.05, 43.62, 26.28, 26.25, 26.13,26.10, 18.90, 18.54, 18.47, -4.49, -4.57, -4.68, -4.69, -5.07, -5.17.

ESI-MS m/z (rel int): (pos) 520 ([M ? Na]?, 100).

5.2 (4S,5R)-10-Hydroxy-4-((triisopropylsilyl)oxy)-5-(((triisopropylsilyl)oxy)-methyl)-10,4,40,5-tetrahydro-3H-spiro[furan-2,30-pyrrolo[2,1-c] [1,4] oxazine]-60-carbaldehyde (12b)

The SN2 coupling between 11 and 14b was similar to the above formation of 12a, however, using Cs2CO3 as mild base [6]. 12b (nearly 1/1 diastereoisomers) was afforded as colorless oil. Yield: 35 mg (60 %).

1H NMR (500 MHz, CDCl3) d 9.53 (s, 1H), 9.40 (s, 1H), 6.91 (s, 1H), 6.40 (d, J = 2.7 Hz, 1H), 6.04 (s, 1H),4.604.57 (m, 1H), 4.143.97 (m, 3H), 3.873.64 (m, 2H),2.552.10 (m, 2H), 1.06 (s, 42H). Isomer A 13C NMR (100 MHz, CDCl3) d 181.27, 132.86, 119.60, 110.68, 107.14, 97.02, 88.65, 75.44, 72.80, 63.92, 41.71, 18.07,12.19, 12.00. Isomer B 13C NMR (100 MHz, CDCl3) d 179.73, 135.64, 120.96, 112.54, 110.68, 107.14, 97.02,88.20, 75.35, 65.39, 43.18, 18.07, 12.19, 12.00. Assigned by comparison with compound 12a, part of

assignments were in some cases interchangeable.

5.3 1-Bromo-1,3-dideoxy-4,6-di-O-(triisopropylsilyl)-

D-fructofuranose (14a)

The modied procedure for bromomethylation was as same as that of 10a [9, 16], 14a (nearly 1/1 diastereoisomers) was afforded as colorless oil. Yield: 32 mg (36 %).

Isomer A 1H NMR (400 MHz, CD3COCD3) d 4.85 (br.s, 1H, OH), 4.574.52 (m, 1H), 4.10 (q, J = 4.4 Hz, 1H), 3.743.71 (m, 2H), 3.45 (d, J = 3.1 Hz, 2H),2.502.44 (m, 1H), 2.031.99 (m, 1H), 1.03 (overlapping s, 42H). 13C NMR (100 MHz, CD3COCD3) d 105.14, 88.06,74.10, 64.04, 45.11, 38.30, 18.35, 12.62.

Isomer B 1H NMR (400 MHz, CD3COCD3) d 5.11 (br.s,

1H, OH), 4.624.58 (m, 1H), 3.94 (q, J = 4.4 Hz, 1H),3.743.71 (m, 2H), 3.67 (m, 1H), 3.52 (dd, J = 10.1,8.2 Hz, 1H), 2.372.32 (m, 1H), 2.252.17 (m, 1H), 1.03 (overlapping s, 42H).13C NMR (100 MHz, CD3COCD3) d 106.00, 89.20, 74.54, 65.11, 44.55, 39.86, 18.35, 12.76.

Assigned by comparison with compound 10a, part of assignments were in some cases interchangeable.

5.4 1,3-Dideoxy-1-iodo-4,6-di-O-(triisopropylsilyl)-D-

fructofuranose (14b)

The procedure for iodomethylation was similar to the reported one [9, 10], 14b (nearly 1/1 diastereoisomers) was afforded as colorless oil. Yield: 41 mg (70 %).

Isomer A 1H NMR (400 MHz, CD3COCD3) d 4.77 (br.s, 1H, OH), 4.594.55 (m, 1H), 4.10 (q, J = 4.4 Hz, 1H), 3.753.73 (m, 2H), 3.36 (s, 2H), 2.492.44(m, 1H),2.142.10 (m, 1H), 1.06 (overlapping s, 42H). 13C NMR (100 MHz, CD3COCD3) d 104.71, 88.36, 74.62, 64.18,45.92, 18.26, 15.41, 12.75, 12.63.

Isomer B 1H NMR (400 MHz, CD3COCD3) d 5.11 (br.s,

1H, OH), 4.624.58 (m, 1H), 3.98 (q, J = 4.4 Hz, 1H),3.753.73 (m, 2H), 3.72 (m, 1H), 3.53 (q, J = 9.7 Hz, 1H),2.402.36 (m, 1H), 2.302.26 (m, 1H), 1.06 (overlapping s, 42H). 13C NMR (100 MHz, CD3COCD3) d 105.49, 89.26,74.67, 65.02, 45.49, 18.26, 12.88, 12.75, 12.63. Assigned by comparison with compound 10a, part of

assignments were in some cases interchangeable.

5.5 Cyanomethyl Cinnamate (18)

To a solution of cinnamic acid (1.48 g, 10 mmol) in CH2Cl2 (4 mL) cooled to 0 C, was added triethylamine(2.02 g, 20 mmol). After stirring 10 min at rt, chloroacetonitrile (1.13 g, 15 mmol) was added and the mixture was stirred overnight. The reaction mixture was quenched with ice-H2O mixture (3 mL) and the aqueous phase was extracted with CH2Cl2 (4 mL 9 2). Combined organic layers were dried over anhydrous Na2SO4 and ltered. After evaporation of the solvents, the residue was puried

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Cascade N-Alkylation/Hemiacetalization for Facile Construction 45

by ash column chromatography to afford 18 as colorless oil [22]. Yield: 1.4 g (75 %).

1H NMR (400 MHz, CDCl3) d 7.80 (d, J = 16.0 Hz, 1H), 7.627.35 (m, 5H), 6.46 (d, J = 16.0 Hz, 1H), 4.86 (s, 2H). 13C NMR (100 MHz, CDCl3) d 165.26, 147.90, 133.77, 131.25, 129.17, 128.53, 115.35, 48.54.

5.6 (E)-1-Hydroxy-7-phenylhept-6-ene-2,5-dione (19)

To a solution of the cyanomethylbenzoate 18 (187 mg, 1 mmol) and Ti(O-i-Pr)4 (313 mg, 1.1 mmol) in dry Et2O (5 mL) was added a solution of EtMgBr (0.7 mL,2.1 mmol, 3 M in Et2O) dropwise at 0 C under nitrogen. After the addition of Grignard reagent, the mixture was allowed to warm up to rt. About 1 h later, the turbid yellow mixture was quenched with water (1 mL). EtOAc (10 mL) and 1 M HCl were added carefully to obtain two clear phases. The aqueous phase was extracted with EtOAc (5 mL 9 2). The combined organic phases were washed with saturated aqueous NaHCO3 and dried by Na2SO4.

After evaporation of the solvents, the residue was puried by ash chromatography to afford 19 as white solid: mp 7274 C [22]. Yield: 153 mg (70 %).

1H NMR (400 MHz, CDCl3) d 7.59 (d, J = 16.3 Hz, 1H), 7.577.54 (m, 2H), 7.417.40 (m, 3H), 6.76 (d, J = 16.2 Hz, 1H), 4.39 (d, J = 4.7 Hz, 2H), 3.11 (t, J = 6.2 Hz, 2H), 3.06 (t, J = 4.8 Hz, 1H), 2.75 (t, J = 6.2 Hz, 2H).13C NMR (100 MHz, CDCl3) d 208.88, 198.00, 143.45, 134.35, 130.84, 129.13, 128.49, 125.60,68.43, 34.33, 31.99.

5.7 (E)-(5-Styryl-1H-pyrrol-2-yl)methanol (20)

A microwave reaction vessel was equipped with a stir bar and charged with diketone 19 (44 mg, 0.2 mmol), NaOAc (20 mg, 0.24 mmol) and NH4OAc (54 mg,0.7 mmol) in 1 mL of EtOH, the resulting mixture in the reaction vessel was sealed and allowed to stir for 5 min at rt, then heated by microwaves at 40 C for 12 min in a CEM-microwave reactor. The reactants were concentrated in vacuo, 1 mL H2O were added, the aqueous phase was extracted with EtOAc (3 mL 9 2), the combined organic layers were dried over anhydrous Na2SO4 and ltered. After evaporation of the solvents, the pure residue 20 was checked by NMR directly. Yield: quantitative conversion.

1H NMR (400 MHz, CDCl3) d 9.17 (s, 1H), 7.417.26 (m, 5H), 6.91 (d, J = 16.4 Hz, 1H), 6.69 (d, J = 16.4 Hz, 1H), 6.23 (s, 1H), 6.11 (s, 1H), 4.61 (s, 2H), 4.19 (s, 1H).

13C NMR (100 MHz, CDCl3) d 137.60, 132.69, 131.67, 128.75, 127.01, 125.95, 123.66, 119.11, 109.32, 108.77,58.03.

Acknowledgments This research was supported by grants from the Hundred Talents Project of Chinese Academy of Science and High-end Science and Technology Talents Program of Yunnan Province (2011HA008). We are grateful to Prof. Derek S. Tan and Dr. Alyssa L. Verano (Memorial Sloan-Kettering Cancer Center and Weill Cornell Medical College) for their valuable discussions and comments.

Conict of interest The authors declare no conict of interest.

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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