Content area
Full Text
Arch Virol (2015) 160:831835 DOI 10.1007/s00705-014-2326-6
BRIEF REPORT
New serotypes of porcine teschovirus identied in Shanghai, China
Huan Sun Han Gao Molin Chen
Daoliang Lan Xiuguo Hua Chunyan Wang
Congli Yuan Zhibiao Yang Li Cui
Received: 22 September 2014 / Accepted: 21 December 2014 / Published online: 1 January 2015 Springer-Verlag Wien 2014
Abstract Teschoviruses are widely endemic and commonly found in pig fecal samples. In this study, we collected fecal specimens from various pig herds and genotyped them based on the VP1 gene. Of 322 samples, 276 were positive, giving a PTV infectivity rate of 85.7 %. PTV4 was the most common serotype found in Shanghai, followed by PTV8 and PTV10. Interestingly, Some Shanghai strains belonging to a new PTV serotype were also isolated. In phylogenetic analysis, PTV SH8 did not correspond to any known serotype. PTV4 and PTV6 showed similar levels of sequence identity to PTV SH8. These data suggest that PTV SH8 is a new serotype, distinct from the new serotype PTV wild boar/WB2C-TV/ 2011/HUN, which clusters with PTV SH2, SH10, and SH25.
Keywords Genetic diversity New serotype Porcine
teschovirus
Introduction
Porcine teschovirus (PTV), previously known as Teschen/ Talfan virus and classied as an enterovirus, now belongs to the genus Teschovirus in the family Picornaviridae [5, 10]. Epidemics of PTV are always accompanied by polioencephalomyelitis, reproductive failure, enteric disease, and pneumonia. Based on a phylogenetic analysis of the viral capsid sequence, PTV can be divided into 11 distinct serotypes, which have been conrmed by indirect immunouorescence assays using monoclonal antibodies [1, 2, 18]. Severe forms of virulent, fatal encephalomyelitis, together with serious economic losses, have been caused by PTV1 [3, 6]. A milder form of the disease can be caused by PTV serotypes 2, 3, 4, 5, 6, 9, and 10 [17]. In China, the disease caused by the prevalent serotype PTV1 has been reported in Inner Mongolia, and PTV2 and PTV8 infections have been identied in Heilongjiang Province [7, 16]. PTV2 infection is accompanied by symptoms of pyrexia, diarrhea, respiratory distress, and nervous disorders, and PTV8 infection causes reproductive failure. PTV4 has also been identied in the north of China [8].
Materials and methods
Specimens
Three hundred twenty-two fecal specimens were collected from two commercial piggeries (153 samples from one piggery...