Content area

Abstract

Macromolecular crowding in cells inuences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells. Here, we introduce a genetically encodable uorescence resonance energy transfer (FRET) sensor for quantifying macromolecular crowding and discuss our application of the sensor in bacterial and mammalian cells.

Details

Title
A sensor for quantification of macromolecular crowding in living cells
Author
Boersma, Arnold J; Zuhorn, Inge S; Poolman, Bert
Pages
227-229
Publication year
2015
Publication date
Mar 2015
Publisher
Nature Publishing Group
ISSN
15487091
e-ISSN
15487105
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1658722994
Copyright
Copyright Nature Publishing Group Mar 2015