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Abstract
A Gram-stain negative, strictly aerobic, rod-shaped, non-motile bacterium, designated strain DY46^sup T^, was isolated from Atlantic Ocean sediment. The isolate was found to grow in medium containing 0-3.0 % (w/v) NaCl (optimally at 0-1.0 %), at 4-37 °C and pH 5.0-8.0. Chemotaxonomic analysis detected MK-6 as the sole isoprenoid quinone. The major fatty acids were identified iso-C^sub 15:0^, iso-C^sub 17:0^ 3-OH, iso-C^sub 17:1^ [omega]9c and summed feature 3 (comprising iso-C^sub 15:0^ 2-OH and/or C^sub 16:1^ [omega]7c). The DNA G + C content was determined to be 40.7 mol %. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain DY46^sup T^ falls within the cluster comprising Chryseobacterium species. The levels of 16S rRNA gene sequence similarity between strain DY46^sup T^ and the type strains of the Chryseobacterium species with validly published names ranged from 92.4 to 99.1 %, the high values (>97 %) being with Chryseobacterium takakiae A1-2^sup T^ (99.1 %), C. taiwanense BCRC 17412^sup T^ (98.0 %), C. taeanense PHA3-4^sup T^ (97.3 %), C. hispalense DSM 25574^sup T^ (97.3 %), C. camelliae THG C4-1^sup T^ (97.2 %), C. gregarium DSM 19109^sup T^ (97.1 %) and C. wanjuense R2A10-2^sup T^ (97.0 %). The DNA-DNA relatedness values between strain DY46^sup T^ and the type strains of the above closely related species were 47, 57, 24, 34, 6, 40 and 21 %, respectively. On the basis of phenotypic and genotypic characteristics, strain DY46^sup T^ represents a novel member within the genus Chryseobacterium, for which the name Chryseobacterium profundimaris is proposed. The type strain is DY46^sup T^ (=CGMCC 1.12663^sup T^ = JCM 19801^sup T^).