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Copyright Nature Publishing Group Aug 2015

Abstract

Chromatin is organized in a highly ordered yet dynamic manner in the cell nucleus, but the principles governing this organization remain unclear. Similarly, it is unknown whether, and how, various proteins regulate chromatin motion and as a result influence nuclear organization. Here by studying the dynamics of different genomic regions in the nucleus of live cells, we show that the genome has highly constrained dynamics. Interestingly, depletion of lamin A strikingly alters genome dynamics, inducing a dramatic transition from slow anomalous diffusion to fast and normal diffusion. In contrast, depletion of LAP2α, a protein that interacts with lamin A and chromatin, has no such effect on genome dynamics. We speculate that chromosomal inter-chain interactions formed by lamin A throughout the nucleus contribute to chromatin dynamics, and suggest that the molecular regulation of chromatin diffusion by lamin A in the nuclear interior is critical for the maintenance of genome organization.

Details

Title
Loss of lamin A function increases chromatin dynamics in the nuclear interior
Author
Bronshtein, I; Kepten, E; Kanter, I; Berezin, S; Lindner, M; Redwood, Abena B; Mai, S; Gonzalo, S; Foisner, R; Shav-tal, Y; Garini, Y
Pages
8044
Publication year
2015
Publication date
Aug 2015
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1706223737
Copyright
Copyright Nature Publishing Group Aug 2015