Content area
Full Text
http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = Dig Dis Sci (2015) 60:33043317 DOI 10.1007/s10620-015-3759-9
http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = ORIGINAL ARTICLE
http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = http://crossmark.crossref.org/dialog/?doi=10.1007/s10620-015-3759-9&domain=pdf
Web End = SATB1 Promotes Pancreatic Cancer Growth and Invasion Depending on MYC Activation
Zheng Chen1,2 Zengliang Li1,2,3,4 Wei Li1,5 Yang Zong1,2,6 Yi Zhu1,2
Yi Miao1,2 Zekuan Xu1,2
Received: 12 March 2015 / Accepted: 10 June 2015 / Published online: 25 June 2015 The Author(s) 2015. This article is published with open access at Springerlink.com
AbstractBackground SATB1 plays an important role in human malignant progression, inducing cancer cell proliferation and metastasis by regulating downstream gene expressions. However, little is known about the underlying mechanisms in which SATB1 promotes pancreatic cancer tumorigenesis.
Aims To investigate SATB1 expression levels and its biological functions in promoting pancreatic cancer growth and invasion.
Methods SATB1 expression levels were detected in seven human pancreatic cancer cell lines and 16 pairs of normal pancreatic/pancreatic cancer tissues using RT-PCR and western blot. SW1990 or Capan-1 cells stably
knockdown (shRNA) or transiently knockdown (siRNA) SATB1 cells, and PANC-1 stably overexpressing SATB1 cells were investigated with MTT, EdU assay, ow cytometry, and transwell invasion assay for cell proliferation and invasion activity. The binding of SATB1 to MYC promoter region was examined using reporter assay. Expression of SATB1 in 68 pancreatic cancer samples was studied by immunohistochemical staining and scoring. Results SATB1 was overexpressed in pancreatic cancer tissues samples, showing strong correlation with pancreatic cancer invasion depth and tumor staging. SATB1 induced MYC mRNA and protein expression; promoted pancreatic cancer cell growth; increased cell population in S phase; and enhanced pancreatic cancer cell invasion in vitro. On the other hand, SATB1 knockdown showed opposite effects. Furthermore, MYC blocking in SATB1-
Zheng Chen and Zengliang Li have been contributed equally to this work.
& Zekuan [email protected]; [email protected]
Zheng Chen [email protected]
Zengliang Li [email protected]
Wei Li [email protected]
Yang Zong [email protected]
Yi Zhu [email protected]
Yi Miao [email protected]
1 Department of General Surgery, The First Afliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, Peoples Republic of China
2 Institute of Tumor Biology, Jiangsu Province Academy of Clinical Medicine, Nanjing, Peoples...