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ABSTRACT
Dihydroquercetin (taxifolin, DHQ) is a natural flavonoid, which possesses antioxidant activity. Recently, in addition to the protective effect of antioxidants against lipid peroxidation, increasing attention is paid to the possibilities of antioxidants, including dihydroquercetin, to prevent oxidation of proteins. Fibrinogen is a plasma protein that serves an important role in clotting. In this work we study the ability of dihydroquercetin to inhibit oxidative modification of fibrinogen. We used ozone as the oxidant. Assessment of functional activity of fibrinogen, both before and after oxidation with ozone, was conducted by determining the time of formation of a fibrin clot after addition of thrombin in a solution of fibrinogen. We found that in the presence of dihydroquercetin ozone oxidation of fibrinogen is negligible, also in the presence of dihydroquercetin fibrinogen retains its functional properties after ozone oxidation, whereas in the case of ozone oxidation of fibrinogen without dihydroquercetin, the functional activity of fibrinogen is completely lost.
Keywords: Dihydroquercetin, taxifolin, oxidative modification of fibrinogen, ozon oxidation, reactive oxygen species
AIM AND BACKGROUND
Oxidative modification of fibrinogen inhibits thrombin-catalyzed clot formation [1], inhibits platelet adhesion and aggregation [2], enhances the activity of tissue plasminogen activator, cause negative effect on hemorheological parameters [3], and is actively involved in the process of inflammation of the vascular wall. Also oxidized fibrinogen is able to induce increased production of interleukin-8 in primary culture of human endothelial cells, which causes chemotaxis of immune cells [4-6]. Oxidation of fibrinogen causes an imbalance in the blood coagulation system.
On the one hand it increases thrombin clotting time and inhibites platelet adhesion and aggregation, on the other hand, it cause formation of neutrophil-platelet aggregates [7]. Thus, the learning of ability of various compounds to prevent oxidative damage to blood plasma proteins, particularly to fibrinogen, is relevant. The objective of this work was to investigate the ability of dihydroquercetin to inhibit the oxidative modification of fibrinogen.
INTRODUCTION
Flavonoids, or bioflavonoids, are a class of plant polyphenols with a wide spectrum of biological activities. These substances give color to plants. Compounds with antioxidant activity are increasingly used in the treatment of diseases in which the induction of lipid peroxidation is one of the key elements of pathogenesis. Such diseases include ischemic attacks, ischemic heart disease, vasculitis of various...