Content area

Abstract

Issue Title: This issue is a translation of Byulleten' Eksperimental'noi Biologii i Meditsiny (Bulletin of Experimental Biology and Medicine) and Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

The content of ATP in scalp hair bulbs in humans was measured in the hair roots from 15 healthy volunteers. Light and electron microscopy confirmed the presence of outer and an inner root sheaths in the root of pulled out anagen hair. Incubation of samples in buffer solution led to extraction of ATP, which was measured by the chemiluminescent method. Mechanic disintegration of hair bulbs and their freezing-defrosting did not increase ATP output. The results of microscopy indicated that ATP extraction procedure was associated with separation of the outer radical sheath from the inner one without impairing the structure of the inner sheath. The mean content of ATP was 12±2 pmol per bulb. The use of pulled out hair bulbs for ATP measurements simplified the procedure as involved no surgical removal of follicles.

Details

Title
Evaluation of ATP Content in Hair Bulbs in Human Scalp
Author
Mikhal'chik, E V; Suprun, M V; Fedorkova, M V; Ibragimova, G A; Dmitrieva, E I; Lipatova, V A; Kutsev, S I
Pages
112-5
Publication year
2014
Publication date
May 2014
Publisher
Springer Nature B.V.
ISSN
00074888
e-ISSN
15738221
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1773234220
Copyright
Springer Science+Business Media New York 2014