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Abstract

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Twenty seven isolates of vancomycin resistant Enterococci based on the disk diffusion and E- test have been screened; being found eight (0.3%) clinical isolates of vanA & vanB through Taq Man Real Time PCR assay. This study shows the presence of both vanA & vanB genotypes in vanA phenotypes clinical isolates in the three hospitals in Iran.

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Key topics
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Polymerase chain reaction;
Rt pcr;
Phosphocreatine;
Reverse transcription polymerase chain reaction;
Iran;
Enterococcus
Subject
Genotype & phenotype;
Microbiology;
Hospitals;
Genotypes
Business indexing term
Location
Iran
Identifier / keyword
vanA & vanB genotypes; Polymerase chain reaction; Rt pcr; Phosphocreatine; Reverse transcription polymerase chain reaction; Iran; Enterococcus; vancomycin; Taq Man Real Time PCR; Enterococci
Title
Detection of both vanA & vanBgenes in vanA phenotypes of Enterococci by Taq Man RT-PCR
Author
Mirzaei, Bahman; Babaei, Ryhane; Asiabar, Akbar Pour Dadash; Bameri, Zakaria
Publication title
Brazilian Journal of Microbiology; São Paulo
Volume
46
Issue
1
Pages
161-165
Publication year
2015
Publication date
2015
Section
Medical Microbiology
Publisher
Springer Nature B.V.
Place of publication
São Paulo
Country of publication
Netherlands
Publication subject
Biology--Microbiology
ISSN
15178382
e-ISSN
16784405
Source type
Scholarly Journal
Language of publication
English
Document type
Feature
Document feature
References
DOI
https://doi.org/10.1590/S1517-838246120131234
ProQuest document ID
1803063458
Document URL
https://www.proquest.com/scholarly-journals/detection-both-vana-amp-vanbgenes-phenotypes/docview/1803063458/se-2?accountid=208611
Copyright
Copyright Sociedade Brasileira de Microbiologia 2015
Last updated
2024-10-04
Database
ProQuest One Academic