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Nature Reviews Genetics | Published online 15 Jul 2016; doi:http://dx.doi.org/10.1038/nrg.2016.94
Web End =10.1038/nrg.2016.94
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A space for transcriptomics
both types of approach. They labelled glass slides with ~200 million reverse transcription oligonucleotide probe molecules: ~1,000 clusters of probes were positioned 200 m apart in a grid, and each cluster included a different spatial barcode within the probes. Initial tests using tissue sections of the mouse olfactory bulb showed that the section could first be imaged by regular haematoxylin and eosin (H&E) staining on the slide, then the tissue could be lysed, the RNA captured on the slide and converted to cDNA with minimal lateral diffusion. The cDNA was then pooled and subjected to RNA-seq in solution. The spatial barcodes allowed the reads to be...