About the Authors:
Sebastián Acosta-Jurado
Affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain
Pilar Navarro-Gómez
Affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain
Piedad del Socorro Murdoch
Affiliation: Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain
Juan-Carlos Crespo-Rivas
Affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain
Shi Jie
Current address: Daqing Branch of Heilongjiang Academy of Sciences, Daqing, P. R. of China
Affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain
Lidia Cuesta-Berrio
Affiliation: Departamento of Química Orgánica, Facultad de Química, Universidad de Sevilla, Sevilla, Spain
José-Enrique Ruiz-Sainz
Affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain
Miguel-Ángel Rodríguez-Carvajal
Affiliation: Departamento of Química Orgánica, Facultad de Química, Universidad de Sevilla, Sevilla, Spain
José-María Vinardell
* E-mail: [email protected]
Affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain
ORCID http://orcid.org/0000-0002-7105-5389
Introduction
Rhizobia are soil α- and β-proteobacteria able to establish a nitrogen-fixing symbiosis with legumes. In this interaction, rhizobia induce the formation of new plant organs, called nodules, on legume roots. Inside nodule cells, rhizobia differentiate into nitrogen fixing bacteroids which provide the plant with ammonia and receive in turns organic acids [1].
The nodulation process relies on a complex molecular dialogue between plant and bacteria [2–4]. The first step is the secretion of phenolic compounds called flavonoids by plant roots. The bacterial protein NodD, upon interaction with appropriate flavonoids, binds to conserved DNA sequences, called nod boxes, and induces the expression of bacterial nod genes, responsible for the synthesis and secretion of molecular signals known as Nod factors. In several rhizobial species, such as Sinorhizobium meliloti and S. fredii, fine-tuning modulation of nod gene expression is achieved by the participation of NolR, a transcriptional repressor protein [5, 6]. Recognition of appropriate Nod factors by LysM plant receptors induces early events of the nodulation process such as root hair curling and nodule primordium formation [7].
In addition to Nod factors, other bacterial molecules are required for a successful interaction [2–4]. Several rhizobia (including S. fredii strains) deliver effector proteins into plant cells through a symbiotic type 3 secretion system in a process that is also dependent on NodD and plant flavonoids [8]. These rhizobial effectors manipulate host cells in order to suppress defensive responses against rhizobia and to promote symbiosis-related processes.
In addition, several rhizobial surface polysaccharides have been demonstrated to play important roles in symbiosis, acting as signals molecules and/or preventing plant defense responses [2, 3, 9]: cyclic glucans (CG), which are cycled polymers of glucose located on the periplasm, lipopolysaccharide (LPS) and K-antigen capsular polysaccharide (KPS), which are constituents of the outer leaf of the external membrane, and exopolysaccharides (EPS), which are acidic polysaccharides located out of the cell with little or no cell association. With the exception of CG, which have been proven to be essential in all rhizobia-legume symbiosis tested so far, the relevance of these polysaccharides depends on the specific rhizobia-legume interaction analyzed [3, 10].
EPS provide protection against different stresses such as desiccation or the presence of antimicrobial compounds and have a role in attachment to surfaces and in biofilm formation [9, 11]. EPS produced by rhizobia are highly variable but mainly contain common monosaccharides such as d-glucose, d-galactose, d-mannose, as well as d-glucuronic and d-galacturonic-acids. In addition, often several non-carbohydrate substituents, such as O-acetyl groups or ketal-linked pyruvate and succinyl half ester groups, are also found. Very recently we have reported the structure of the S. fredii HH103 EPS, which is composed of glucose, galactose, glucuronic acid, pyruvic acid (5:2:2:1) and is partially acetylated [12]. Rhizobial EPS is essential in several interactions with legumes forming indeterminate nodules, such as Sinorhizobium meliloti-Medicago or Rhizobium leguminosarum bv. trifolii-Trifolium [11 and references therein], but this is not always the case. Thus, an exoA (EPS-) mutant of S. fredii HH103 is slightly impaired but still effective in its interaction with Glycyrrhiza uralensis or Cajanus cajan [10, 13]. In addition, this S. fredii HH103 exoA mutant is fully effective with Glycine max (soybean) or Vigna unguiculata [13, 14], two legumes forming determinate nodules, and it even shows increased competitiveness to nodulate soybean [12].
Several reports indicate that nod gene inducing flavonoids, such as apigenin or genistein, can affect the production of S. fredii surface polysaccharides. Thus, in strain NGR234 the presence of apigenin reduces the amount of KPS produced and elicits the synthesis of a novel kind of LPS rich in rhamnose in a NodD-dependent manner [15, 16]. None of these changes have been found in S. fredii HH103 [14, 17] or, to our knowledge, in other S. fredii strains. In 1992, Dunn et al. [18] reported that genistein provokes alterations in the molecular mass and the composition of S. fredii USDA193 EPS. More recently, we reported that both the presence of this flavonoid and the inactivation of nolR results in a non-mucoid phenotype in S. fredii HH103 [6].
In this work we have further investigated the relation between the nod regulatory network and EPS production in S. fredii HH103. We show that the effect of flavonoids on EPS production is related to their nod gene induction ability and, moreover, that it is absolutely dependent on NodD1. The non-mucoid phenotype is due to a decrease in EPS production and not to structural changes. This reduced EPS production is, at least partially, related to a decrease in the level of transcripts of the exoY2 and exoK genes in stationary phase cultures, although the exact way in which this expression is modulated remains to be elucidated.
Material and Methods
Microbiological techniques
Sinorhizobium fredii strains HH103 RifR [19], SVQ116 (= HH103 RifR nodA::Tn5-B20, [20]), SVQ530 (= HH103 RifR exoA::lacZΔp::GmR [13]), SVQ502 (= HH103 RifR nodD1::lacZΔp::GmR [21]), SVQ548 (= HH103 RifR nolR::lacZΔp::GmR, this work), and SVQ554 (= HH103 RifR nodD2::lacZΔp::GmR, this work) were grown at 28°C on TY medium [22], yeast extract/mannitol (YM) medium [23] or MGM medium [24]. Plasmids pMUS296 and pMUS746 are pMP92 derivatives containing the nodD1 and the nodD2 genes of S. fredii HH103, respectively [21, 25]). The nolR and nodD2 mutant derivatives of HH103 used in this work were constructed by homogenotization of a copy of the gene truncated by the lacZΔp-GmR cassette [26] following methodologies previously used by our group [10, 13, 14, 21]. S. fredii strains USDA257 [27] and NGR234 [28] and S. meliloti Rm1021 [29] were cultured on YM medium. Escherichia coli was cultured on Luria-Bertani (LB) medium [30] at 37°C. When required, the media were supplemented with the appropriate antibiotics as described by Vinardell et al. [21]. Flavonoids were dissolved in ethanol at a concentration of 1 mg/mL and used at 1 μg/mL. Plasmids were transferred from E. coli to rhizobia by triparental mating by using pRK2013 as the helper plasmid [31]. The Optical Density (OD) of bacterial cultures was determined by using a Pharmacia LKB Novaspec II spectrophotometer.
For analysis of EPS production in solid media, 20 μl droplets of YMB-grown early log (OD600 = 0.4) cultures were placed onto YMA plates supplemented with ethanol or flavonoids (flavone, genistein, or luteolin), incubated at 28°C for 96 hours and photographed. For EPS quantification, bacterial cultures were grown in YM for 96 hours at 28°C (OD600 = 1.2–1.3) under shaking conditions. Cells were removed by centrifugation (20000 g, 15 min) and total carbohydrate amounts of the EPS-containing supernatants were determined using the anthrone-H2SO4 method, which measures the total glucose equivalents content in a given sample, as previously described [32]. Four independent experiments in duplicate were carried out. Assays for biofilm formation on plastic surfaces were carried out as described by Rodríguez-Navarro et al. [12]. Data presented are the media of at least three independent experiments performed in duplicate; in each experiment, at least 12 wells for each treatment were measured.
Molecular techniques
Recombinant DNA techniques were performed according to the general protocols of Sambrook et al. [30]. PCR amplifications and hybridisations were performed as previously described [6]. For measuring expression of the exoA, exoK, exoY2 and exoA genes, quantitative RT-PCR experiments were performed by using primer pairs rt-exoA-F2/R2, rt-exoK-F/R, exoY2rt-F/R, and qnodA-F/R respectively. The S. fredii HH103 16S rRNA was used as an internal control to normalize gene expression (primer pair rt-16S-F2/R2). All primer pairs used are shown in S1 Table. Total RNA was isolated using the High Pure RNA Isolation Kit (Roche) and RNase Free DNA Set (Qiagen) according to the manufacturer’s instructions. This (DNA free) RNA was reverse transcribed to cDNA by using PrimeScript RT reagent Kit with gDNA Eraser (Takara). Quantitative PCR was performed using a LightCycler 480 (Roche, Switzerland) with the following conditions: 95°C, 10 min; 95°C, 30 s; 50°C, 30 s; 72°C, 20 s; forty cycles, followed by the melting curve profile from 60 to 95°C to verify the specificity of the reaction. The fold changes of three biological samples with three technical replicates in each condition were obtained using the ΔΔCt method.
Statistical analyses
For each strain and condition, the capacity of biofilm formation, the amount of glucose equivalents produced and the expression level of nodA and exo genes were compared to those of the wild type strain grown in the absence of genistein by using the Mann-Whitney non-parametrical test.
Chemical analyses of EPS
For isolation of bacterial EPS, culture media were concentrated up to 20% of the initial volume on a rotary evaporator and three volumes of cold ethanol were added. After 24 h at 4°C, the resulting precipitates were separated by centrifugation, redissolved in water and purified by dialysis against distilled water. Finally, the solutions were concentrated and freeze dried. For NMR experiments, 1–5 mg of samples were deuterium-exchanged several times by freeze-drying from D2O and then examined in solution (1–5 mg/750 mL of 99.96% D2O). Spectra were recorded at 353 K on a Bruker AV500 spectrometer operating at 500.20 MHz (1H). Chemical shifts are given in ppm, using the HDO signal (4.22 ppm at 353 K) as reference [33].
Results
Sinorhizobium fredii HH103 EPS production is regulated by nod gene inducing flavonoids
Previous results of our research group had shown that the mucoidy of S. fredii HH103 in YM medium is negatively affected by the presence of genistein, a flavonoid present in soybean root exudates that is an effective nod gene inducer of this strain [6]. This result prompted us to investigate whether this effect also took place in other S. fredii strains. The ineffective nod gene inducer flavone [21] and ethanol (the organic solvent used to dissolve flavonoids) were used as negative controls of the effect of genistein. In addition to S. fredii strains, S. meliloti Rm1021 was also included in this experiment since it has been reported that flavonoids has a slight inducer effect on EPS I production in this strain [34]. For this reason, in addition to genistein, we also investigated the effect of luteolin, a Medicago sativa root-exuded flavonoid that is an effective nod gene inducer for both S. meliloti and S. fredii [21, 35].
Results presented in Fig 1 indicate that, among the different S. fredii strains tested, the flavonoid effect on EPS production appears to be specific for HH103. Thus, S. fredii HH103 presented a rough appearance when grown in YMA plates supplemented with genistein or luteolin, whereas the mucoidy of strains USDA257 and NGR234 was apparently not affected by the presence of these flavonoids. The slight positive effect of luteolin on EPS I production by Rm1021 could not be noticed by this approach. The appearance of S. fredii HH103 was not altered in YMA plates supplemented with either ethanol or flavone, indicating that neither the organic solvent used to dissolve flavonoids nor flavonoids ineffective as nod gene inducers affect S. fredii HH103 mucoidy.
[Figure omitted. See PDF.]
Fig 1. Mucoidy of different sinorhizobial strains in YMA medium in the presence or absence of flavonoids.
Compounds used for supplementing YMA medium are indicated on the left.
https://doi.org/10.1371/journal.pone.0160499.g001
Flavonoid-mediated regulation of S. fredii HH103 EPS depends on the regulatory genes nodD1 and nolR
Since only those flavonoids effective as nod inducers have a negative effect on S. fredii HH103 EPS production, we decided to investigate whether there is a connection between the nod regulon and EPS production in S. fredii HH103. For this purpose we analyzed the effect of genistein on the mucoidy of the wild type strain as well as of HH103 derivatives affected in nodA (unable to produce Nod factors) and in different nod regulatory genes present in this strain [6, 17, 21, 36]: nodD1, nodD2, and nolR (Fig 2). Strain SVQ530, an HH103 exoA derivative defective in EPS production [12], was employed as negative control for EPS production.
[Figure omitted. See PDF.]
Fig 2. Mucoidy of S. fredii HH103 (wt) and different mutant derivatives in YMA in the absence or presence of flavone or genistein.
The gene affected by the mutation in each strain is indicated on the top of each column. Plasmids pMUS296 and pMUS746 harbor wild-type copies of HH103 nodD1 and nodD2 respectively. Compounds used for supplementing YMA medium are indicated on the left. Control denotes absence of flavonoids.
https://doi.org/10.1371/journal.pone.0160499.g002
As expected, regardless the presence or absence of flavonoids, HH103 exoA showed a rough appearance. The presence of genistein, but not that of flavone, led to a decrease in mucoidy in the wild type strain and in its nodD2 and nodA derivatives. Interestingly, in the absence of flavonoids or in the presence of flavone, the appearance of the nodA derivative was different to that of the wild type since disruptions in the mucoidy could be observed in the former strain. As previously described [6], lack of NolR led to a less mucoid phenotype of S. fredii HH103 even in the absence of flavonoids, although this phenotype was more evident in the presence of genistein. In the nodD1 mutant background the presence of genistein did not affect HH103 mucoidy. Consistently, introduction of the wild-type nodD1 gene (harboured by plasmid pMUS296) restored the negative effect of genistein on mucoidy in the nodD1 mutant and even reduced those of the wild type strain and its nodD1 mutant in the absence of genistein. These results indicate that the genistein-mediated repression of HH103 mucoidy involves the participation of NodD1. However, introduction of extra copies of nodD2 (carried by plasmid pMUS756) also reduced mucoidy in the wild type strain in the absence of flavonoids, suggesting that, when in overdose, NodD2 could mimic the mucoidy repression exerted by NodD1.
Production of EPS by S. fredii HH103 and its nodA, nodD1, and nolR mutant derivatives in the absence or presence of genistein was further investigated by quantification of the amount of glucose equivalents found in the extracellular milieu of cultures of these strains in YM broth (YMB) (Fig 3A). The HH103 exoA derivative was used as a negative control for production of EPS. The low levels of glucose equivalents detected in HH103 exoA might correspond to other polysaccharides present in culture supernatants such as cyclic glucans. The presence of genistein reduced about 10-fold the amount of glucose equivalents produced by HH103 (to levels similar to that of the exoA mutant) but did not affect that of the HH103 nodD1 derivative. In addition, the HH103 nolR derivative clearly produced less amount of glucose equivalents than the wild type strain, regardless the presence or absence of genistein. In the absence of flavonoids, the nodA derivative of HH103 produced similar amounts of glucose equivalents when compared to the wild type strain. Surprisingly, when genistein was present, the amount of glucose equivalents present in the extracellular milieu of HH103 nodA was significantly enhanced in comparison to those of the non-induced cultures of both the wild-type and the nodA mutant strains.
[Figure omitted. See PDF.]
Fig 3.
Glucose equivalents in the extracellular milieu (A) and biofilm formation ability (B) of S. fredii HH103 (wt) and its nodA, nodD1, nolR and exoA mutant derivatives grown in the presence (+) or absence (-) of genistein. The presence of an asterisk denotes a significant difference at the 0.01% level with respect to wt (-).
https://doi.org/10.1371/journal.pone.0160499.g003
In S. fredii HH103 EPS has been shown to be essential for biofilm formation ability onto plastic surfaces [12]. Because of this, we have investigated whether this bacterial capacity is also affected by the presence of genistein. The exoA derivative of HH103 was employed as negative control for biofilm formation. As shown in Fig 3B, the presence of genistein reduced dramatically biofilm formation ability in the wild-type but not in the nodD1 mutant strain. The HH103 nolR derivative formed less amount of biofilm than the wild-type strain in the absence of flavonoids, and was also negatively affected by the presence of genistein. However, the decrease in biofilm formation ability exhibited by this mutant was not as dramatic as the reduction in EPS production. This result might be due to the fact that NolR is a global regulator, so that its absence might affect other traits involved in bacterial attachment. The biofilm formation ability of the HH103 nodA derivative was similar to that of the parental strain, both in the absence or presence of genistein.
NMR experiments confirm that genistein diminishes S. fredii HH103 EPS production in a NodD1-dependent manner
Polysaccharides present in culture media were isolated and their 1H-NMR spectra acquired. Fig 4 shows that when HH103 is grown in the absence of genistein (wt-), the isolate contains mainly its exopolysaccharide [12], together with minor components such as a mannan from yeast extract or small amounts of KPS [37]. Its nodD1 derivative has the same behavior, either in the presence or the absence of genistein (nodD1- and nodD1+, respectively). Spectra from genistein-induced cultures of HH103 (wt+) and its nolR derivative (nolR+), on the contrary, do not show signals from EPS. These signals are very weak in non-induced cultures of HH103 nolR (nolR-). In these cases, 1H-NMR spectra present mainly signals from mannan and KPS, indicating that the amounts of EPS have been drastically reduced.
[Figure omitted. See PDF.]
Fig 4. 1H-NMR (500.20 MHz, 353 K) of the polysaccharides isolated from YMB culture supernatants of S. fredii HH103 (wt) and its nodD1 and nolR derivatives grown in the absence (-) or presence of genistein (+).
Peaks marked with asterisks correspond to a mannan present in yeast extract, one of the components of YMB medium.
https://doi.org/10.1371/journal.pone.0160499.g004
Thus, NMR analyses confirmed that genistein reduces S. fredii HH103 production of EPS only when a functional copy of nodD1 is present, and that the absence of NolR also provokes a diminution of the amount of EPS produced. Altogether, our results indicate that in HH103 the production of EPS is regulated in an opposite manner to that of Nod factors and Nops (Nodulation Outer Proteins): it is repressed by NodD1 and flavonoids, which are required for the expression of genes involved in Nod factors and Nops production, but enhanced by the regulatory protein NolR, which in turns represses the expression of nod and nop genes [6].
Genistein promotes a reduction of the expression of exoK and exoY2 in S. fredii HH103 stationary phase cultures
The observed genistein-mediated repression of EPS production by S. fredii HH103 prompted us to investigate whether the presence of this flavonoid may have an effect on the expression of genes related to EPS production in this strain. Very recently, we have carried out a transcriptomic analysis of the effect of genistein on global gene expression in S. fredii HH103 [data are available at http://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA313151]. These studies, which were carried out upon a 24 h genistein treatment (early-stationary phase), did not revealed significant changes in the expression level of exo and exs genes (│fold changes│≤ 1.6), which are those involved in the production of EPS [12, 17].
Since S. fredii HH103 production of EPS is only evident in late-stationary phase, we decided to investigate the level of expression of several exo genes upon 96 hours of genistein treatment. The organization of the exo and exs genes of S. fredii HH103, which are clustered in a region of the largest plasmid (pSfHH103e) has been recently described [12, 17]. For our studies, we selected the exoA, exoY2, and exoK genes. Although biochemical studies of the enzymatic activities of the products encoded by these genes have not been carried out in S. fredii HH103, their high level of identity with the corresponding S. meliloti proteins (whose activities have been established, revised in [11]) clearly suggest that they most probably have the same role. Thus, ExoA would be involved in the addition of the first glucose residue to the lipid-galactose structure of the nascent EPS repeating unit, ExoY could participate in the addition of the first sugar to the lipid carrier, and ExoK could act as a glucanase involved in the cleavage of high- (HMW) to low-molecular-weight form of EPS.
For the analysis of exo gene expression, quantitative RT-PCR (qPCR) were done by using cDNA from early (24 hours) or late (96 hours) stationary phase cultures of S. fredii HH103 and its nodD1 and nolR derivatives grown in the presence or absence of genistein (Fig 5). In addition to exoA, exoK and exoY2, the nodA gene was also studied as a control of a gene induced by NodD1 and flavonoids and repressed by NolR. As expected, the expression of nodA was strongly induced by genistein when a functional NodD1 protein was present (wild-type), being this effect enhanced by the absence of NolR (nolR mutant background). After 24 hours of growth, the levels of expression of exoA, exoK and exoY2 in the nodD1 and nolR mutant backgrounds were not different from those of the wild-type strain, both in the absence or presence of genistein. Upon 96-hours of genistein treatment, the expression of exoA was neither affected by the presence of flavonoids in the three strains analyzed. In contrast, both exoK and exoY2 showed reduced expression when both NodD1 and genistein were present (induced cultures of the wild-type and nolR mutant strains). This effect was stronger in the case of exoY2, whose expression level was 5-fold and 10-fold reduced in HH103 and in the nolR mutant background respectively. In the case of the nolR mutant, this reduction in the expression level of exoY2 was observed both in the presence and absence of genistein.
[Figure omitted. See PDF.]
Fig 5. qRT-PCR analysis of the effect of genistein in the expression level of exoA, exoK and exoY2 expression in S. fredii HH103 and its nodD1 and nolR mutant derivatives in early (24 h) and late (96 h) stationary phase cultures.
(-) and (+) denote the absence and presence of genistein respectively. The presence of an asterisk denotes a significant difference at the 1% level with respect to the expression level in the wild type strain grown in the absence of genistein.
https://doi.org/10.1371/journal.pone.0160499.g005
Discussion
The rhizobia-legume nitrogen-fixing symbiosis is based on the interchange of signals between both symbionts [2–4]. It is well established that flavonoids exuded by legume roots interact with the bacterial NodD protein inducing the expression of nod genes, which are responsible for the production and secretion of bacterial Nod factors. These molecular signals, in turns, elicit root hair curling and the formation of nodule primordia and are also required for infection thread formation. In some rhizobia, including S. fredii strains, flavonoids and NodD also induce the expression of ttsI, which codes for the transcriptional activator of a symbiotic type III secretion system responsible for the delivery of bacterial effector proteins into host plant cells (revised in [8]).
In S. fredii NGR234, flavonoids are known to also influence surface polysaccharides production. Thus, the presence of apigenin (an effective nod gene inducer for this strain) reduces the amount of K-antigen capsular polysaccharide produced in a process mediated by nodD1 and y4gM [15] and leads to the production of a rhamnose-rich LPS through the induction of the fixF, rgpF and wbgA genes [16]. None of these changes takes place in S. fredii HH103, since y4gM, fixF, rgpF, and wbgA are not present in this strain [17].
In this work, we report that nod gene inducing flavonoids such as genistein reduces HH103 EPS production in a NodD1-dependent manner. To our knowledge, this is the first time that this mechanism of EPS repression is reported in rhizobia. Previous works showed that in S. fredii USDA193, genistein provoked changes in EPS production [18], but these changes were structural (lower average molecular mass and reduced uronic acids contents compared to control) and, although they were pSym-dependent, it has not been studied whether they were mediated by NodD proteins. In S. fredii USDA191 the presence of extra copies of nodD2 leaded to rough colony morphology whereas extra copies of nodD1 only reduced very slightly production of EPS in this strain [38, 39]. In addition these changes did not depend on the presence of flavonoids. In S. fredii HH103, the absence of nodD1 but not that of nodD2 led to a mucoid phenotype in the presence of flavonoids, indicating that the repressor effect of flavonoids in this strain is mediated by NodD1. However, the presence of plasmid pMUS746 in the wild-type strain also reduced its mucoidy in the presence of flavonoids, indicating that, when overexpressed, NodD2 is also able to repress EPS production in HH103. The recent sequencing of the S. fredii HH103 genome has revealed the presence of 15 putative nod boxes [17], but none of them is located upstream of genes directly involved in the biosynthesis of EPS (exo and exs genes). Therefore, the effect of NodD1 and genistein on S. fredii HH103 EPS production most probably is mediated by other regulatory proteins which remain to be identified.
In contrast to the results presented in this work, in both S. meliloti Rm1021 and Rhizobium leguminosarum bv. trifolii 24.2, the presence of flavonoids has been shown to enhance EPS production [34, 40]. In Rm1021 this effect is mediated by the transcriptional regulator SyrM, which is connected to the nod regulon through NodD3 and that stimulates EPS production through SyrA [41, 42]. In S. fredii strains syrM is present, but both nodD3 and syrA are missing [17, 43]. R. leguminosarum bv. trifolii lacks a copy of syrM, and in strain 24.2 the effect of flavonoids contained in clover root exudates on EPS production is exerted though the RosR transcriptional regulator [40]. In S. fredii HH103 there are two orthologues of RosR, called MucR1 and MucR2 [17, 43]. We are currently analyzing the role of the S. fredii HH103 syrM, mucR1, and mucR2 genes of the effect of flavonoids on EPS production in this strain. Our preliminary results show that HH103 mucR1 is an activator of EPS production, which is in agreement with recent results obtained for another S. fredii strain, CCBAU45436 [44], but it is not connected with the effect exerted by flavonoids. With respect to S. fredii HH103 syrM, most probably transcription of this gene is dependent on nod box 19 and activated by NodD1 and genistein [17], what would make this gene a good candidate for EPS regulation. Another HH103 gene that could be involved in EPS regulation is pSfHH103d_161 [17], whose expression is most probably driven by nod box 10. This gene codes for a putative protein that is 58% identical to R. leguminosarum biovar phaseoli PsiB, an EPS repressor which has not been studied in other rhizobia [45]. Further research is required to elucidate whether syrM and/or pSfHH103d_161 could be involved in the genistein induced repression of EPS production. In any case, results presented in this paper as well as previous results of our group [6] demonstrate that this effect is exerted through the nod regulon and that it involves NodD1 and NolR as a repressor and an activator respectively of EPS production, indicating that in S. fredii HH103 EPS is regulated in just the opposite way that Nod factors and type 3 secreted effectors, which are induced by NodD1 and flavonoids and repressed by NolR [6, 21, 25]. Regardless what other genes participate in this regulatory network, the presence of genistein and/or the lack of NolR led to a significant decrease of the expression of the HH103 exoK and exoY2 genes as demonstrated in this work by quantitative RT-PCR. Our results are in agreement with previous observations carried out in S. meliloti and R. leguminosarum bv. trifolii, in which modulation of EPS production is achieved by regulating the expression of a small number of exo/pss genes [46, 47]. Remarkably, in the case of S. meliloti two of these genes are exoK and exoY [46].
Another interesting finding found in this study is the fact that the nodA mutant derivative of S. fredii HH103, when compared to the parental strain, showed alterations in its appearance when grown on YMA plates as well as in the amount of glucose equivalents found in the extracellular milieu in the presence of genistein. Elucidating the nature of these alterations is an issue that we will address in the next future. Remarkably, inactivation of nodA, which results in inability to produce Nod factors, does not alter the HH103 ability to attach onto plastic surfaces. In contrast, in S. meliloti Nod factors are required for the establishment of the three-dimensional architecture of biofilms [48], which suggests that different sinorhizobial strains might have developed different strategies for biofilm formation.
EPS plays an essential role in nodule invasion in symbiosis with legumes forming indeterminate nodules, such as the interactions S. meliloti-Medicago or Rhizobium leguminosarum bv. trifolii-Trifolium [11 and references therein]. On the other hand, although traditionally EPS has been considered not essential in symbiosis forming determinate nodules, recent works show that production of altered forms of this polysaccharide can lead to impairments in the interactions Bradyrhizobium japonicum USDA110-soybean and Mesorhizobium loti-Lotus japonicus [49, 50]. These results can be explained by the recent discovery of a Lotus LysM receptor which senses the presence of EPS and which is able to block root infection when non-appropriate forms of this polysaccharide are detected [51].
So, an open question is why S. fredii HH103 EPS production is repressed by flavonoids present in legume root exudates? S. fredii HH103 has a broad host range of nodulation [52], which allows studying the role of its molecular signals with different host legumes. Regarding surface polysaccharides, only CG has been proven to be essential for nodulating all its host plants tested so far [53]. In previous works, we have analyzed the symbiotic performance of an EPS deficient derivative of this strain due to an exoA mutation [10, 13, 14]. This mutant was able to induce nitrogen fixing nodules in the different legumes analyzed regardless they formed indeterminate (Glycyrrhiza uralensis and Cajanus cajan) or determinate (Glycyne max and Vigna unguiculata) nodules, suggesting a low importance for this polysaccharide in HH103 symbiotic ability. In fact, with soybean (G. max) the HH103 exoA derivative not only showed a slightly increased symbiotic performance but it was even more competitive for nodulating this plant [12, 13], which suggest that, at least with this legume, the presence of EPS can have a slight but detrimental effect on symbiosis. However, this would not be always the case, since opposite results were found with cowpea (V. unguiculata), in which the exoA derivative of S. fredii HH103 showed slightly decreased symbiotic performance and lower competitiveness ability than the parental strain [12, 13].
In summary, we show that in S. fredii HH103 nod gene inducing flavonoids, in addition to promote the production of Nod factors and the delivery of type 3 secreted effectors, modify bacterial surface by repressing EPS production. This change is different to those described in other rhizobia such as S. fredii NGR234, in which flavonoids repress KPS and induce a novel type of LPS, or S. meliloti and R. leguminosarum bv. trifolii, which increase EPS production upon treatment with flavonoids. Thus, this study represents a new example of that mechanisms found in model bacteria are not always present in other rhizobial strains.
Supporting Information
[Figure omitted. See PDF.]
S1 Table. Primers used in quantitative RT-PCR (qPCR) experiments.
https://doi.org/10.1371/journal.pone.0160499.s001
(DOC)
Acknowledgments
We thank the Biology and NMR Services of the Centro de Investigación, Tecnología e Innovación (CITIUS) of the University of Seville. We would also like to thank to the two anonymous referees that have reviewed this work since their suggestions have undoubtedly contributed to improve its quality.
Author Contributions
1. Conceived and designed the experiments: JMV SAJ JERS MARC.
2. Performed the experiments: SAJ PNG PSM JCCR SJ LCB.
3. Analyzed the data: SAJ JMV JCCR PSM MARC.
4. Contributed reagents/materials/analysis tools: JMV JERS MARC.
5. Wrote the paper: JMV.
Citation: Acosta-Jurado S, Navarro-Gómez P, Murdoch PdS, Crespo-Rivas J-C, Jie S, Cuesta-Berrio L, et al. (2016) Exopolysaccharide Production by Sinorhizobium fredii HH103 Is Repressed by Genistein in a NodD1-Dependent Manner. PLoS ONE 11(8): e0160499. https://doi.org/10.1371/journal.pone.0160499
1. Suzaki T, Yoro E, Kawaguchi M. Leguminous plants: inventors of root nodules to accommodate symbiotic bacteria. Int Rev Cell Mol Biol. 2015; 316: 111–158. pmid:25805123
2. Downie JA. The roles of extracellular proteins, polysaccharides and signals in the interactions of rhizobia with legume roots. FEMS Microbiol Rev. 2010; 34: 150–170. pmid:20070373
3. Janczarek M, Rachwał K, Marzec A, Grządziel J, Palusińska-Szysz M. Signal molecules and cell-surface components involved in early stages of the legume–rhizobium interactions. Appl Soil Ecol. 2015; 85: 94–113.
4. López-Baena FJ, Ruiz-Sainz JE, Rodríguez-Carvajal MA, Vinardell JM. Bacterial molecular signals in the Sinorhizobium fredii-soybean symbiosis. Int J Mol Sci. 2016; 17:755.
5. Kondorosi E, Gyuris J, Schmidt J, John M, Duda E, Hoffmann B, et al. Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation. EMBO J. 1989; 8: 1331–1340. pmid:16453883
6. Vinardell JM, Ollero FJ, Hidalgo A, López-Baena FJ, Medina C, Ivanov-Vangelov K, et al. NolR regulates diverse symbiotic signals of Sinorhizobium fredii HH103. Mol Plant-Microbe Interact 2004; 17: 676–685. pmid:15195950
7. Oldroyd GE. Speak, friend, and enter: signalling systems that promote beneficial symbiotic associations in plants. Nat Rev Microbiol. 2013; 11: 252–263. pmid:23493145
8. Staehelin C, Krishnan HB. Nodulation outer proteins: double-edged swords of symbiotic rhizobia. Biochem J. 2015; 470: 263–274. pmid:26341483
9. Fraysse N, Couderc F, Poinsot V. Surface polysaccharide involvement in establishing the Rhizobium-legume symbiosis. Eur J Biochem. 2003; 270: 1365–1380. pmid:12653992
10. Margaret-Oliver I, Lei W, Parada M, Rodríguez-Carvajal MA, Crespo-Rivas JC, Hidalgo A, et al. Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume. Arch Microbiol. 2012; 194: 87–102. pmid:21761170
11. Janczarek M. Environmental signals and regulatory pathways that influence exopolysaccharide production in rhizobia. Int J Mol Sci. 2011; 12: 7898–7933. pmid:22174640
12. Rodríguez-Navarro DN, Rodríguez-Carvajal MA, Acosta-Jurado S, Soto MJ, Margaret I, Crespo-Rivas JC, et al. Structure and biological roles of Sinorhizobium fredii HH103 exopolysaccharide. PLoS One. 2014; 9: e115391. pmid:25521500
13. Parada M, Vinardell JM, Ollero FJ, Hidalgo A, Gutiérrez R, Buendía-Clavería AM, et al. Sinorhizobium fredii HH103 mutants affected in capsular polysaccharide (KPS) are impaired for nodulation with soybean and Cajanus cajan. Mol Plant-Microbe Interact. 2006; 19: 43–52. pmid:16404952
14. Hidalgo A, Margaret I, Crespo-Rivas JC, Parada M, Murdoch PS, López A, et al. The rkpU gene of Sinorhizobium fredii HH103 is required for bacterial K-antigen polysaccharide production and for efficient nodulation with soybean but not with cowpea. Microbiology 2010; 156: 3398–3411. pmid:20688828
15. Simsek S, Ojanen-Reuhs T, Marie C, Reuhs BL. An apigenin-induced decrease in K-antigen production by Sinorhizobium sp. NGR234 is y4gM- and nodD1-dependent. Carbohydr Res. 2009; 344: 1947–1950. pmid:19679303
16. Ardissone S, Noel KD, Klement M, Broughton WJ, Deakin WJ. Synthesis of the flavonoid-induced lipopolysaccharide of Rhizobium sp. strain NGR234 requires rhamnosyl transferases encoded by genes rgpF and wbgA. Mol Plant-Microbe Interact. 2011; 24: 1513–1521. pmid:22066901
17. Vinardell JM, Acosta-Jurado S, Zehner S, Göttfert M, Becker A, Baena I, et al. The Sinorhizobium fredii HH103 genome: a comparative analysis with S. fredii strains differing in their symbiotic behavior with soybean. Mol Plant Microbe Interact. 2015; 28: 811–824. pmid:25675256
18. Dunn MF, Pueppke SG, Krishnan HB. The nod gene inducer genistein alters the composition and molecular mass distribution of extracellular polysaccharides produced by Rhizobium fredii USDA193. FEMS Microbiol Lett. 1992; 97: 107–112.
19. Madinabeitia N, Bellogín RA, Buendía-Clavería AM, Camacho M, Cubo T, Espuny MR, et al. Sinorhizobium fredii HH103 has a truncated nolO gene due to a -1 frameshift mutation that is conserved among other geographically distant S. fredii strains. Mol Plant Microbe Interact. 2002; 15: 150–159. pmid:11878319
20. Buendía-Clavería AM, Moussaid A, Ollero FJ, Vinardell JM, Torres A, Moreno J, et al. A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide. Microbiology. 2003; 149: 1807–1818. pmid:12855732
21. Vinardell JM, López-Baena FJ, Hidalgo A, Ollero FJ, Bellogín R, del Rosario Espuny M, et al. The effect of FITA mutations on the symbiotic properties of Sinorhizobium fredii varies in a chromosomal-background dependent manner. Arch Microbiol. 2004; 181: 144–154. pmid:14689165
22. Beringer JE. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974; 84: 188–198. pmid:4612098
23. Vincent JM. Appendix III. The modified Fåhraeus slide technique. In Vincent JM, editor. A Manual for the Practical Study of Root Nodule Bacteria. Oxford, UK. Blackwell Scientific, 1970. pp 144–145.
24. O’Toole GA, Kolter R. Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol. 1998; 28: 449–461. pmid:9632250
25. López-Baena FJ, Vinardell JM, Pérez-Montaño F, Crespo-Rivas JC, Bellogín RA, Espuny MR, et al. Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103. Microbiology. 2008; 154: 1825–1836. pmid:18524937
26. Becker A, Schmidt M, Jäger W, Pühler A. New gentamicin resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Gene. 1995; 162: 37–39. pmid:7557413
27. Keyser HH, Bohlool BB, Hu TS, Weber DF. Fast-growing rhizobia isolated from root nodules of soybean. Science. 1982; 215: 1631–1632 pmid:17788491
28. Trinick MJ. Relationships amongst the fast-growing rhizobia of Lablab purpureus, Leucaena leucocephala, Mimosa sp., Acacia farnesiana and Sesbania grandiflora and their affinities with other rhizobial groups. J Appl Bacteriol. 1980; 49: 39–53
29. Meade HM, Signer ER. Genetic mapping of Rhizobium meliloti. Proc Natl Acad Sci USA. 1977; 74: 2076–2078. pmid:266730
30. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1989.
31. Simon R. High frequency mobilization of gram-negative bacterial replicons by the in vivo constructed Tn5-Mob transposon. Mol Gen Genet. 1984; 196: 413–420. pmid:6094969
32. Abarca-Grau AM, Burbank LP, de Paz HD, Crespo-Rivas JC, Marco-Noales E, López MM, et al. Role for Rhizobium rhizogenes K84 cell envelope polysaccharides in surface interactions. Appl Environ Microbiol. 2012; 78: 1644–1651. pmid:22210213
33. Gottlieb HE, Kotlyar V, Nudelman A. NMR Chemical shifts of common laboratory solvents as trace impurities. J Org Chem. 1997; 62: 7512–7515. pmid:11671879
34. Dusha I, Olah B, Szegletes Z, Erdei L, Kondorosi A. syrM is involved in the determination of the amount and ratio of the two forms of the acidic exopolysaccharide EPS I in Rhizobium meliloti. Mol Plant Microbe Interact. 1999; 12: 755–765.
35. Peters NK, Frost JW, Long SR. A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes. Science 1986; 233: 977–980. pmid:3738520
36. Machado D, Pueppke SG, Vinardell J.M, Ruiz-Sainz JE, Krishnan HB. Expression of nodD1 and nodD2 in Sinorhizobium fredii, a nitrogen-fixing symbiont of soybean and other legumes. Mol Plant Microbe Interact. 1998; 11: 375–382.
37. Gil-Serrano AM, Rodríguez-Carvajal MA, Tejero-Mateo P, Espartero JL, Menendez M, Corzo J, et al. Structural determination of a 5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)-l-glycero-l-manno-nonulosonic acid-containing homopolysaccharide isolated from Sinorhizobium fredii HH103. Biochem J. 1999; 342: 527–535. pmid:10477263
38. Appelbaum ER, Thompson DV, Idler K, Chartrain N. Bradyrhizobium japonicum USDA191 has two nodD genes that differ in primary structure and function. J Bacteriol. 1988; 170: 12–20. pmid:2826389
39. Machado D, Krishnan HB. nodD alleles of Sinorhizobium fredii USDA 191 differentially influence soybean nodulation, nodC expression, and production of exopolysaccharides. Curr Microbiol. 2003; 47: 134–137. pmid:14506861
40. Janczarek M, Skorupska A. Modulation of rosR expression and exopolysaccharide production in Rhizobium leguminosarum bv. trifolii by phosphate and clover root exudates. Int. J. Mol. Sci. 2011; 12: 4132–4155. pmid:21747729
41. Barnett MJ, Swanson JA, Long SR. Multiple genetic controls on Rhizobium meliloti syrA, a regulator of exopolysaccharide abundance. Genetics. 1998; 148: 19–32. pmid:9475718
42. Barnett MJ, Long SR. The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3. J Bacteriol. 2015; 197: 1792–1806. pmid:25777671
43. Weidner S, Becker A, Bonilla I, Jaenicke S, Lloret J, Margaret I, et al. Genome sequence of the soybean symbiont Sinorhizobium fredii HH103. J Bacteriol. 2012; 194: 1617–1618. pmid:22374952
44. Jiao J, Wu LJ, Zhang B, Hu Y, Li Y, Zhang XX, et al. MucR Is Required for Transcriptional Activation of Conserved Ion Transporters to Support Nitrogen Fixation of Sinorhizobium fredii in Soybean Nodules. Mol Plant Microbe Interact. 2016; 29: 352–361. pmid:26883490
45. Mimmack ML, Borthakur D, Jones MA, Downie JA, Johnston AW. The psi operon of Rhizobium leguminosarum biovar phaseoli: identification of two genes whose products are located at the bacterial cell surface. Microbiology. 1994; 140: 1223–1229. pmid:7517767
46. Keller M, Roxlau A, Wenig WM, Schmidt M, Quandt J, Niehaus K, et al. Molecular analysis of the Rhizobium meliloti mucR gene regulating the biosynthesis of the exopolysaccharides succinoglycan and galactoglucan. Mol Plant-Microbe Interact. 1995; 8: 267–275. pmid:7756693
47. Rachwał K, Matczyńska E, Janczarek M. Transcriptome profiling of a Rhizobium leguminosarum bv. trifolii rosR mutant reveals the role of the transcriptional regulator RosR in motility, synthesis of cell-surface components, and other cellular processes. BMC Genomics. 2015; 16: 1111. pmid:26715155
48. Fujishige NA, Lum MR, De Hoff PL, Whitelegge JP, Faull KF, Hirsch AM. Rhizobium common nod genes are required for biofilm formation. Mol Microbiol. 2008; 67: 504–515. pmid:18086203
49. Quelas JI, Mongiardini EJ, Casabuono A, López-García SL, Althabegoiti MJ, Covelli JM. et al. Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean. Mol Plant Microbe Interact. 2010; 23: 1592–1604. pmid:20636103
50. Kelly SJ, Muszyński A, Kawaharada Y, Hubber AM, Sullivan JT, Sandal N, et al. Conditional requirement for exopolysaccharide in the Mesorhizobium-Lotus symbiosis. Mol Plant Microbe Interact. 2013; 26: 319–329. pmid:23134480
51. Kawaharada Y, Kelly S, Nielsen MW, Hjuler CT, Gysel K, Muszyński A, et al. Receptor-mediated exopolysaccharide perception controls bacterial infection. Nature 2015; 523: 308–312. pmid:26153863
52. Margaret I, Becker A, Blom J, Bonilla I, Goesmann A, Göttfert M, et al. Symbiotic properties and first analyses of the genomic sequence of the fast growing model strain Sinorhizobium fredii HH103 nodulating soybean. J. Biotechnol. 2011; 155: 11–19. pmid:21458507
53. Crespo-Rivas JC, Margaret I, Hidalgo A, Buendía-Clavería AM, Ollero FJ, López-Baena FJ, et al. Sinorhizobium fredii HH103 cgs mutants are unable to nodulate determinate- and indeterminate nodule-forming legumes and overproduce an altered EPS. Mol Plant Microbe Interact. 2009; 22: 575–588. pmid:19348575
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
© 2016 Acosta-Jurado et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Abstract
In the rhizobia-legume symbiotic interaction, bacterial surface polysaccharides, such as exopolysaccharide (EPS), lipopolysaccharide (LPS), K-antigen polysaccharide (KPS) or cyclic glucans (CG), appear to play crucial roles either acting as signals required for the progression of the interaction and/or preventing host defence mechanisms. The symbiotic significance of each of these polysaccharides varies depending on the specific rhizobia-legume couple. In this work we show that the production of exopolysaccharide by Sinorhizobium fredii HH103, but not by other S. fredii strains such as USDA257 or NGR234, is repressed by nod gene inducing flavonoids such as genistein and that this repression is dependent on the presence of a functional NodD1 protein. In agreement with the importance of EPS for bacterial biofilms, this reduced EPS production upon treatment with flavonoids correlates with decreased biofilm formation ability. By using quantitative RT-PCR analysis we show that expression of the exoY2 and exoK genes is repressed in late stationary cultures of S. fredii HH103 upon treatment with genistein. Results presented in this work show that in S. fredii HH103 EPS production is regulated just in the opposite way than other bacterial signals such as Nod factors and type 3 secreted effectors: it is repressed by flavonoids and NodD1 and enhanced by the nod repressor NolR. These results are in agreement with our previous observations showing that lack of EPS production by S. fredii HH103 is not only non-detrimental but even beneficial for symbiosis with soybean.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer