Caloric restriction improves health and survival

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Received 31 May 2016 | Accepted 24 Nov 2016 | Published 17 Jan 2017

Julie A. Mattison1,*, Ricki J. Colman2,*, T. Mark Beasley3,4,*, David B. Allison3, Joseph W. Kemnitz2,5, George S. Roth6, Donald K. Ingram7, Richard Weindruch8,9, Rafael de Cabo1,** & Rozalyn M. Anderson8,9,**

Caloric restriction (CR) without malnutrition extends lifespan and delays the onset of age-related disorders in most species but its impact in nonhuman primates has been controversial. In the late 1980s two parallel studies were initiated to determine the effect of CR in rhesus monkeys. The University of Wisconsin study reported a signicant positive impact of CR on survival, but the National Institute on Aging study detected no signicant survival effect. Here we present a direct comparison of longitudinal data from both studies including survival, bodyweight, food intake, fasting glucose levels and age-related morbidity. We describe differences in study design that could contribute to differences in outcomes, and we report species specicity in the impact of CR in terms of optimal onset and diet. Taken together these data conrm that health benets of CR are conserved in monkeys and suggest that CR mechanisms are likely translatable to human health.

DOI: 10.1038/ncomms14063 OPEN

Caloric restriction improves health and survival of rhesus monkeys

1 Translational Gerontology Branch, National Institute on Aging, Baltimore, Maryland 21224, USA. 2 Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA. 3 Department of Biostatistics, University of Alabama, Birmingham, Alabama 35294, USA. 4 Geriatric Research Education and Clinical Center, Birmingham/Atlanta Veterans Administration Hospital, Birmingham, Alabama 35233, USA. 5 Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, Wisconsin 53792, USA. 6 GeroScience, Pylesville, Maryland 21323, USA. 7 Pennington Biomedical Research Center, Baton Rouge, Louisiana 70808, USA. 8 Department of Medicine, University of Wisconsin, Madison, Wisconsin 53792, USA.

9 Geriatric Research Education and Clinical Center, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53705, USA. * These authors contributed equally to this work. ** These authors jointly supervised this work. Correspondence and requests for materials should be addressed to R.d.C. (email: mailto:[email protected]

Web End [email protected] ) or to R.M.A. (email: mailto:[email protected]

Web End [email protected] ).

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The rhesus monkey (Macaca mulatta) is an excellent model for human ageing. The rhesus monkey genome shares B93% sequence identity with the human genome1,2, and

numerous aspects of their anatomy, physiology, neurology, endocrinology and immunology directly parallel those of humans3,4. Rhesus monkeys have a lifespan measured in decades, and develop, mature and age in similar ways to humans. In terms of ageing this includes greying and thinning of hair, redistribution of body fat, loss of skin tone, loss of vigour and loss of muscle tone47. With age there are increases in clinical manifestations of diseases and disorders that also increase in prevalence with advancing age in humans, including diabetes, neoplasia, sarcopenia, bone loss, altered immune function and cognitive decline3,4,8. Like humans, nonhuman primates are genetically heterogeneous so that phenotypes of ageing are non-uniformly manifested among individual animals. Variance can be somewhat offset through comprehensive life-long physical and medical health records, where age-related changes are viewed from the perspective of each animal individually. Nonhuman primates exhibit feeding patterns and sleeping behaviour similar to those of humans, and a key advantage of nonhuman primate studies over human studies is that all variables including the environment and dietary intake can be fully dened. In short, nonhuman primates are vital models for translating basic research into clinical application.

A clear understanding of the biology of ageing, as opposed to the biology of individual age-related diseases, could be the critical turning point for novel approaches in preventative strategies to facilitate healthy human ageing. Caloric restriction (CR) offers a powerful paradigm to uncover the cellular and molecular basis for the age-related increase in overall disease vulnerability that is shared by all mammalian species. CR extends median and maximum lifespan in most strains of laboratory rodents and also delays the onset of age-related diseases and disorders912. Lifespan is also extended by CR in most short-lived species, including the unicellular yeast, nematodes and invertebrates. There has been rapid progress in identifying potential mechanisms of CR utilizing these models1317. These short-lived species are well suited for the investigation of the underlying mechanisms of CR due to the relative ease in their genetic manipulation, extensive genetic and developmental characterization, low cost, and signicantly reduced timeframe for completion of longevity studies. A key question underpinning this body of work is whether the biology of CR, and its ability to delay ageing and the onset of disease, applies to humans and human health.

To date three independent studies of rhesus monkeys (Macaca mulatta) have tackled the question of translatability of CR to primate species. The University of Maryland rhesus monkey study was the rst to report a positive association of CR with survival with a 2.6-fold increased risk of death in control animals compared to restricted18. The primary focus of the study was not

CR however, and analysis was based on comparing 109 ad libitum fed males and females from colony records at that facility, including insulin resistant and diabetic animals, to only eight male CR monkeys. Two other studies focused specically on the impact of CR in healthy male and female rhesus monkeys: one at the National Institute on Aging (NIA) involving 121 monkeys; and the other at the University of Wisconsin Madison (UW) with 76 monkeys. The same University of Alabama at Birmingham based statistical team was engaged for analysis of data from both studies. The UW study has reported benecial effects of CR, including signicant improvements in health and age-related survival19, and all-cause survival20. In contrast, the NIA study reported no signicant impact of CR on survival, although improvements in health were close to statistical signicance21. The basis for the contrasting outcomes from these two parallel studies has not been established. Analysis of limited published bodyweight data indicated that the controls were not equivalent between the two studies20, pointing to fundamental differences in study design and implementation. Therefore, to more fully assess possible explanations for the discrepant ndings between the two studies, we have conducted a comprehensive assessment of longitudinal data from both sites highlighting differences that may have contributed to the dissimilar outcomes.

ResultsIntrinsic differences in study design. Most of the early rodent CR studies involved very young onset life-long CR initiated post-weaning, usually in inbred genetic backgrounds. In the 1980s it became clear that adult onset CR (12-month-old mice) was also effective in delaying ageing and extending lifespan in rodents, albeit to a lesser extent than the young onset model22. Many rodent CR studies opt to feed control animals ad libitum amounts of food while others provide less than ad libitum amounts arguing that this strategy avoids the confounding effects of obesity and reduces variability in food intake among individuals. With the launch of the NIA rhesus monkey study in 1987, the implementation of CR was such that the control monkeys were not free-fed. Food allotments were determined in accordance with data published by the National Research Council to provide approximate ad libitum intake based on their age and bodyweight for the maturing control monkeys without overfeeding23. Rations were increased to maintain growth and development until full stature was attained. CR monkeys received 30% less food than height-, age- and sex-matched control monkeys. The intervention was initiated as young-onset and old-onset groups of males, and young, adult, and old-onset groups of females24 (Table 1). Launched in 1989, the UW study initiated the CR diet in adult animals only, after full stature was achieved (B8 years of age for rhesus monkeys)25. Food was provided at levels approximating ad libitum to control animals. To accommodate heterogeneity in the feeding behaviours within the cohort, the ad libitum reference

Table 1 | Study design.

Site Group Control N CR N Age of onset Genetic origin NIA Male Juvenile 10 10 12 years Indian/Chinese

Adolescent 12 10 35 years Indian/Chinese Old 10 10 1623 years Indian/Chinese Female Juvenile 9 9 13 years Indian

Adult 15 11 614 years Indian/Chinese Old 8 7 1621 years Indian

UW Male 23 23 714 years Indian Female 15 15 915 years Indian

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for each individual was established using baseline food intake measured over 36 months, and CR was implemented on a per-individual basis. The rationale for these design features at UW was to implement a study as it might have been conducted in humans.

The source of the monkeys in each cohort and the population type represented is also a point of difference for the two studies. The UW monkeys were born and raised at the Wisconsin National Primate Research Center and were all of Indian origin. The NIA monkeys were sourced from several locations and included monkeys of both Indian and Chinese origin. Chinese male rhesus monkeys are generally heavier and longer than their Indian counter parts with the reverse being the case for females, and Chinese rhesus monkeys are also thought to exhibit greater sexual dimorphism26. Monkeys of different origin are sufciently genetically different that they can be distinguished using a panel of single nucleotide polymorphisms27. Apart from population differences, rhesus monkeys share a similar degree of inter-individual genetic variation as humans28. In this way, the contribution of population type to differences in outcomes of the two studies as opposed to the contribution of individual genetic heterogeneity is difcult to ascertain.

The diet compositions were another important difference between the two studies. First, the source of diet components was different. A naturally sourced diet was employed at the NIA facility to ensure that micronutrients such as phytochemicals and trace minerals were provided, acknowledging that there was potential for seasonal variation. In contrast, a semi-puried diet was employed at UW to ensure that intake could be fully dened and consistent throughout the course of the study. Second, although diets at both locations had a similar caloric density, the relative macronutrient composition of the diets was not equivalent (Table 2). Compared to the UW diet, the NIA diet was lower in fat, higher in protein and higher in bre. Finally, the nutrient content of the diets was also different. At both locations diets containedB60% carbohydrates by weight, but sucrose comprised less than 7% of total carbohydrates at NIA and 45% of total carbohydrates at UW. Diets at both locations were replete for vitamins that were provided at or above the recommended daily allowance.

Feeding practices also differed between studies. At NIA, the monkeys were fed two meals at B6:30 and 13:00 each day. Any food remaining after the morning meal was removed after about 3 h, and a low calorie treat was provided, typically in the form of a small piece of fruit. The afternoon meal was not removed so that monkeys had access to food at night. At UW, all monkeys were fed in the morning atB8:00 and any remaining food was removed at B16:00 when a treat of fresh fruit or vegetable, which was quickly and completely eaten, was provided. Food allotment for control animals was adjusted to ensure that there was always some uneaten food to be removed at the end of the day. In this way UW animals were ad libitum fed during the day but food

deprived overnight. While there were considerable differences in study design as outlined above, it should be noted that animal housing and routine animal care were equivalent at NIA and UW primate facilities. This included identical housing conditions, temperature and humidity range, light cycles, and the use of tap water, which was continuously available. Both studies included animal monitoring several times per day, and a designated veterinary staff that inspected the animals routinely and provided outstanding care as needed.

Impact of CR on survival. The initial goal of both NIA and UW studies was to determine the impact of CR on the health of rhesus monkeys, as it was not a foregone conclusion that CR would be an appropriate intervention in long-lived species. The investigation of the impact of CR on longevity was not considered a primary outcome at either study location. Even though 121 monkeys were enrolled in the NIA study, the differences in age of onset (from 1 to 23 years) precluded the animals from being grouped together for data analyses. Although the age range for time of onset is smaller for the UW study (ages 715), with only 38 outbred genetically distinct monkeys per group (including both sexes), it seemed unlikely that the study would have the statistical power required to test CRs effect on longevity. While neither study reports longevity data, both studies have yielded survival data. For rhesus monkeys in captivity, the previously reported median survival was B26 years of age, 10% survival was B35 years of age and maximal survival was B40 years of age29. Mortality curves were generated separately for UW and NIA (Fig. 1). Survival estimates for monkeys at both sites were calculated based on data captured up to July 2015 using the three most common statistical methods: Kaplan-Meier product-limit method; Cox proportional hazard regression and parametric survival analysis assuming a Weibull distribution (Table 3). Because the Weibull distribution is a special case of the generalized extreme value distribution, it can accommodate estimation of the upper quantiles of a survival distribution and maximal lifespan, especially when there are censored data due to animals that remain alive30.

In the UW adult-onset study, the estimated survival of UW control animals was close to that of the average recorded for monkeys in captivity (B26 years of age). Considering both males and females together, a statistically signicant effect of CR in increasing survival was observed (Cox regression P 0.017;

Supplementary Table 1). The hazard ratio (HR) of 1.865 (95% condence interval (CI): 1.1193.108) indicated that at any time-point the control monkeys had almost twice the rate of death when compared to CR animals. The effect of sex on the response to CR was not statistically signicant. Kaplan-Meier analysis showed that median survival estimates were greater for CR animals for both males and females (Table 3). In the NIA study large differences in ages of monkeys at time of recruitment to the

Table 2 | Diet composition at each location.

Diet component NIA UWControl/CR (% by weight)

Nutrient sources Control(% by weight)

CR(% by weight)

Nutrient sources

Protein 17.3 soybean and sh meal 13.13 13.13 lactalbuminCarbohydrate 56.9 wheat, corn, sucrose (6.8%) 60.92 58.31 corn, sucrose (45%), dextrin Fat 5.0 soy, corn, sh oils 10.6 10.6 corn oilFibre 6.59.0 cellulose 5.0 5.0 celluloseVitamins B140% RDA 100% RDA B130% RDA

Calories kcal g 1 3.9 3.9 3.8

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Control: 2 CR: 5

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Figure 1 | Mortality curves for monkeys at UW and at NIA. These curves depict data for male and female monkeys on the UW study and on the NIA study. Animals are grouped by age where male J/A include juvenile and adolescent onset animals, female J/A include juvenile and adult onset animals, and old include the advanced age onset animals. Inset boxes indicate animals still alive, dashed line marks 50% mortality. Statistics related to this gure are provided in Supplementary Information, Supplementary Table 1.

Table 3 | Survival estimates.

Groups KM KM KM KM Weibull Weibull

Median IQR Mean SE Median IQR

All cause survival estimates

Males UW Control 26.11 6.82 25.28 1.03 25.75 5.57

CR 28.32 6.19 26.86 1.23 27.63 8.04 NIA J/A Control 28.78 NE 26.00 1.50 29.22 14.31

CR 26.31 13.06 23.71 1.91 25.86 17.05 NIA old Control 34.78 9.16 34.19 1.89 34.80 7.23

CR 37.10 10.42 34.82 1.95 35.88 8.71

Females UW Control 23.86 7.92 23.56 1.29 24.57 8.62

CR 29.68 15.60 25.78 2.18 27.49 14.37 NIA J/A Control 25.67 12.65 24.58 1.71 25.81 14.21

CR 22.63 NE 19.79 1.20 23.53 15.25 NIA old Control 27.19 8.98 28.57 1.59 29.71 8.91

CR 27.87 11.88 26.13 2.65 26.49 8.80

KM, Kaplan-Meier; IQR, interquartile range.

study (Table 1) prompted a separation of data from the early and late onset groups. Here and throughout this report, NIA male juveniles and adolescents (J/A) were grouped and female juveniles and adults were grouped (J/A). The Kaplan-Meier median estimated survival was not different between NIA control and CR animals for the J/A onset groups of males or females (Fig. 1).

Although Cox proportional hazard regression indicated that the differences in survival between J/A control and CR were not statistically signicant (Supplementary Table 1), CR monkeys reached 80% mortality before the controls for both sexes. With 38% of the NIA J/A cohort still alive, the survival curves are incomplete and the impact on survival remains to be determined;

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however, the early mortality suggests that for some individuals implementation of CR in the very young may confer a survival risk. For old-onset CR, Kaplan-Meier estimated survival was not different between control and CR groups for either males or females (Table 3), but survival estimates were higher than those of J/A monkeys and UW controls. For both males and females, survival estimates for the NIA old-onset cohort were comparable to or exceeded those for UW CR.

Although there were slight discrepancies in the estimated median survival between the non-parametric Kaplan-Meier and parametric Weibull estimation methods, the survival comparisons between study sites using either analysis were consistent. A certain degree of sexual dimorphism was observed in survival outcomes where incidence of early death appeared to be greater for females. This observation might be explained in part by endometriosis, which is the proliferation of endometrial tissue outside of the uterus. Endometriosis can occur at relative high incidence in monkeys in captivity (B25%), and risk is considerably greater for nulliparous females31,32. Incidence of endometriosis was equivalent for control and CR groups. For the J/A cohorts in the NIA study, 12 of the 44 females died of complications due to endometriosis, and of these the juvenile onset females were conrmed nulliparous. Females recruited to the UW study, in contrast, had at least one but no more than three healthy infants33, and only 2 of 30 females died of

complications due to endometriosis. A further contributing factor relates to the policy on treatment of clinical conditions. At UW the policy to treat clinical conditions was implemented from the outset. At NIA, although acute pain and suffering were always treated, chronic medical conditions, including endometriosis, were monitored but not medically treated. A policy change was implemented in 2010 due to the high incidence of endometriosis. The power to assess the impact of CR on survival for NIA J/A females has been compromised somewhat by this one condition.

Biometric and food intake measures from both studies. For over a quarter of a century during these studies, bodyweight, body composition and food intake were measured for all 197 monkeys. Bodyweight was determined in fasted and anesthetized monkeys 24 times per year during routine procedures. Longitudinal data for all monkeys were averaged by age of the animal (Fig. 2a). As is the case for humans, monkeys often experience cachexia or end-of-life rapid weight loss. To avoid confounding effects of weight change that is not related to food intake or diet, data from the last year of life for each monkey were excluded. To facilitate comparisons among the cohorts, data were grouped into three age categories representing young adult (1113 years of age), late mid-age (1820 years of age) and advanced age (2527 years of age) (Supplementary Tables 2 and 3).

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NIA J/A control NIA J/A CRFigure 2 | Bodyweight data for monkeys at NIA and UW. (a) Bodyweight (kg) for male and female monkeys at UW and at NIA grouped by age where male J/A include juvenile and adolescent onset animals, female J/A include juvenile and adult onset animals, and old include the advanced age onset animals. Digits shown in white within the boxes are the numbers of individual animals contributing to each data point, data are shown as means.e. of the mean. (b) Comparison of bodyweight averages for monkeys from UW and NIA studies with records of the internet Primate Aging Database (iPAD). Average bodyweight for control and CR monkeys at both study locations were determined by age category including adult (1113 years of age), late mid-age (1820 years of age) and advanced age (2527 years of age). Data are expressed as percent deviation from the iPAD average for females and males from each age category. Statistics related to this gure are provided in Supplementary Information, Supplementary Tables 2 and 3.

UW control NIA old control UW CR NIA old CR

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Considering rst the female monkeys, bodyweight for the NIA J/A was not signicantly different between control and CR monkeys for any age categories. UW CR females weighed signicantly less (1726%) than controls throughout the study period, and UW female controls weighed signicantly more than NIA J/A female controls throughout (Supplementary Table 2). For NIA old-onset females, bodyweight was not signicantly different between controls and CR, and was signicantly lower than bodyweight of UW female controls. In summary, for NIA J/A and old-onset female cohorts, bodyweight for control and CR monkeys was not different from each other and all were signicantly lower than the UW controls. Considering next the male monkeys, NIA J/A CR males weighed signicantly less (1922%) than their control counterparts throughout the study. The difference between UW control and CR was slightly greater (2435%), with CR males weighing signicantly less than controls. The average peak weight for NIA J/A control males was B15% lower than that of UW control males, but differences in bodyweight were signicant for the young age category only (Supplementary Table 3). Bodyweight of the old-onset NIA control and CR males were not signicantly different at either mid-age or advanced ages, and old-onset NIA male controls weighed signicantly less than UW controls. In summary, NIA J/A and UW male cohorts showed a clear bodyweight response to CR, but old-onset NIA control and CR males were not different from each other and were signicantly lower than the UW controls.

The internet Primate Aging Database (iPAD; http://ipad.primate.wisc.edu

Web End =http://ipad. http://ipad.primate.wisc.edu

Web End =primate.wisc.edu ) is a repository of clinical and biometric data from healthy, non-experimental, captive nonhuman primates housed at research facilities across the USA. Using data from over 1,200 individual rhesus monkeys of Indian origin, mean bodyweights were calculated for the above age categories for males(11.6, 12.1, 11.5 kg respectively) and females (7.4, 8.4, 7.8 kg respectively). UW control and CR monkeys fell on either side of these averages; control monkeys were heavier than the iPAD

average (B18% for males; B19% for females), and CR monkeys had lower bodyweight than the iPAD average (B12% for males; B11% for females) (Fig. 2b). For NIA J/A, control males were the same to slightly heavier (510%) than the iPAD average and CR weighed less than the iPAD average (B20%), while control and

CR female monkeys both weighed less than the iPAD average throughout the study (B10% and B20% respectively). All NIA old-onset monkeys weighed less than the iPAD average for both control (B15% for females; B10% for males) and CR (B22% for females; B21% for males) monkeys. In summary, bodyweights of UW and NIA control monkeys were not equivalent to each other, and apart from J/A males, were respectively higher and lower of the iPAD average.

To gain insight into differences in the effect of age and diet on body composition, dual X-ray absorptiometry measures were conducted at intervals throughout the course of the two studies (Fig. 3). Since each animal had multiple measures taken over time, estimates of the average percent adiposity (fat/bodyweight expressed as percent) were adjusted for age (Supplementary Fig. 1). Within groups a main effect of age on adiposity was detected for NIA J/A and UW cohorts. A main effect of diet was detected for NIA J/A males and for both males and females from the UW study, where CR was associated with signicantly lower adiposity. The NIA J/A control and CR females did not differ from each other in adiposity and neither of the NIA old-onset monkey groups had a main effect of CR on adiposity. Combining the data from NIA J/A and UW, a difference in adiposity was detected between controls on the two studies for both males and females, where NIA monkeys had signicantly lower percent body fat. Control monkeys from NIA J/A were not statistically different from UW CR in percent body fat for both sexes. These data show an impact of age on adiposity in all three groups and reveal that the impact of CR on adiposity was observed for both groups of UW monkeys and at NIA for J/A males only.

Food intake was monitored daily at both sites. At UW daily measures of food intake were used to calculate means. At NIA

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Figure 3 | Adiposity data for female and male monkeys at NIA and UW. Percent adiposity (fat (g)/total bodyweight (g)) calculated from DXA(dual energy X-ray absorptiometry) measures conducted during the course of the studies for male and female monkeys at UW and at NIA grouped by age where male J/A include juvenile and adolescent onset animals, female J/A include juvenile and adult onset animals, and old include the advanced age onset animals. Digits shown in white within the boxes are the numbers of individual animals contributing to each data point, data are shown as means.e. of the mean.

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750

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Figure 4 | Food intake data for monkeys at NIA and UW. Food intake (daily values in Kcalories) for male and female monkeys at UW and at NIA grouped by age where male J/A include juvenile and adolescent onset animals, female J/A include juvenile and adult onset animals, and old include the advanced age onset animals. Digits shown in white within the boxes are the numbers of individual animals contributing to each data point,data are shown as means.e. of the mean.

food intake means were calculated based on measures conducted during a single week per year as representative of typical intake. Longitudinal data for all monkeys were averaged by age of the animal (Fig. 4). Data from the last year of life of each monkey were excluded to avoid confounding effects of end-of-life feeding behaviours that usually include loss of appetite. Considering rst the females and using the age categories dened above for both UW and NIA J/A, the controls consumed signicantly more calories than CR at both young and mid-age, but the difference persisted only for UW female monkeys at advanced age. For the old-onset NIA, caloric intake was not different between control and CR. Among control monkeys, UW females consumed signicantly more calories than NIA J/A at mid-age and advanced age and more than old-onset at advanced age. Considering next the males, the NIA J/A controls consumed signicantly more calories than CR at young and mid-age and the difference between control and CR was signicant for UW at mid-age only. Old-onset males at NIA differed signicantly in their caloric intake between control and CR only at advanced age. Among controls, caloric intake was not different for NIA J/A and UW males at any point in the study, but old-onset males consumed signicantly less than UW males and NIA J/A males at mid-age. In summary, signicant differences in caloric intake were identied between control and CR monkeys for male and female NIA J/A and UW cohorts, but not for old-onset cohorts until advanced age and then for males only. Comparing between sites, caloric intake for NIA female controls of both J/A and old-onset was lower than that of UW controls, and for males, caloric intake of NIA J/A and UW controls were not different from each other but old-onset NIA controls were lower than both.

Impact of CR on incidence of disease. The concept of health-span is a fairly recent development in ageing research, where a distinction is drawn between chronological age and health status34. Traditionally, an increase in both median and maximum lifespan was considered the hallmark of delayed ageing, and improvements in health were deemed to be a necessary and obvious component of longevity. The perspective has shifted somewhat towards greater emphasis on health and morbidity, so

an intervention that imparts improved health even in the absence of increased longevity, is viewed as a highly favourable and legitimate example of an ageing intervention. With advancing age, rhesus monkeys are vulnerable to many of the same conditions observed in humans. Among the most prevalent are cancer, cardiac disease, and conditions related to immune dysfunction and inammation, and examples of each were identied in monkeys on the ageing and CR studies at both NIA and UW (Supplementary Table 4).

Fasting glucose measures were common to both studies and the longitudinal data are shown (Fig. 5). In healthy adult rhesus monkeys fasting glucose levels are 6468 mg dl 1 (refs 18,35).

For NIA J/A, fasting glucose levels were equivalent for controls and CR up to B23 years of age, after which the control and CR males, but not females, began to diverge. Both control and CR females showed an age-related increase in fasting glucose levels after B21 years of age. For UW monkeys, the control males had higher fasting glucose levels than CR from 15 years of age with a further divergence of the curves after B23 years of age, while a noticeable difference between control and CR females emerged after only B21 years. For the NIA old-onset cohorts, fasting glucose was consistently low for the duration of the study period. These data point to an age-related increase in fasting glucose for rhesus monkeys and single out the UW control males as being predisposed to elevated circulating glucose in the fasted state. Using multilevel modelling to investigate the relationship between adiposity and fasting glucose levels a signicant relationship was identied for UW males only (P 0.005).

A signicant age by diet interaction was also detected (P 0.014),

suggesting that the impact of age on the relationship between adiposity and glucoregulatory parameters is distinct for control and CR monkeys.

Veterinarians documented body condition and overall health of monkeys biannually at both study locations and indicators of diseases or disorders identied. The age at which a monkey was rst diagnosed with an age-related condition was used to generate morbidity curves (Fig. 6). Age-related conditions included sarcopenia, osteoporosis, arthritis, diverticulosis, cataracts and persistent heart murmurs, in addition to age-related diseases including cancer, diabetes and cardiovascular

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Figure 5 | Fasting glucose values for monkeys at NIA and UW. Circulating levels of glucose (mg dl 1) are shown for male and female monkeys at UW and at NIA grouped by age where male J/A include juvenile and adolescent onset animals, female J/A include juvenile and adult onset animals, and old include the advanced age onset animals. Digits shown in white within the boxes are the numbers of observations contributing to each data point, data are shown as means.e. of the mean.

disease. Cox proportional hazard regression modelling indicated that age-related conditions occurred at B2.7 times the rate in control animals compared to CR for UW monkeys (HR: 2.665; CI: 1.5274.653; P 0.0006). In the NIA J/A cohort, age-related

conditions occurred at twice the rate in control monkeys compared to CR (HR: 2.091; CI: 1.1693.641; P 0.0125)

(Supplementary Table 5; Supplementary Fig. 2). The advanced age of the old-onset NIA monkeys precluded detection of the rst occurrence of an age-related condition.

The incidence of age-related conditions prevalent in human populations, such as cancer, cardiovascular disease and glucoregulatory dysfunction/diabetes, was determined for monkeys at UW and NIA, with J/A and old-onset combined. Diagnoses were made clinically by veterinary staff upon presentation, and disease-related pathology was subsequently conrmed upon necropsy by a board-certied pathologist. Clinically silent pathologies were identied at necropsy. To compare studies, cancer and cardiovascular disorders are reported as incidence upon necropsy. Adenocarcinoma was the leading cause of death at both study sites, consistent with previous reports on cancer incidence in rhesus monkeys36,37. The incidence of adenocarcinoma or other less common neoplasms was lower in CR monkeys at both locations (Fig. 6). The most common diagnosis of cardiovascular disease in live animals was mitral valve dysfunction, while valvular endocardiosis, cardiomyopathy and myocardial brosis were detected at necropsy. Incidence of cardiovascular disorders was lower in CR monkeys than controls at UW. There was no apparent impact of diet on incidence of cardiovascular disease for monkeys at NIA; however, incidence for both control and CR monkeys was lower than UW controls. Assessment of glucoregulatory function was part of routine

clinical care at both locations, although clinical denitions representing different disease stages were employed at the two sites. Similar to humans38,39, insulin resistance occurs in advance of impaired fasting glucose in rhesus monkeys35,40, which occurs before transition to full diabetes. At UW loss of insulin sensitivity was used to diagnose glucoregulatory impairment and was dened as fasting insulin levels (470 mU ml 1) and an insulin sensitivity index (Si)o2(E 04) as determined by a frequently sampled intravenous glucose tolerance test. At NIA, fasting glucose (4100 mg dl 1), glucosuria and HbA1c (46.5%)

measures were used to dene diabetes. CR animals had lower incidence of glucoregulatory dysfunction than controls at both UW and NIA study sites. Multilevel modelling was used to investigate possible relationships between adiposity and morbidity but no signicant effects were detected for any group from either study. Similarly, for all NIA groups and for UW males, no relationship between adiposity and mortality was detected. For UW females, adiposity was associated with a modest reduction in risk for death (HR: 0.927; P 0.01) but only after

correcting for age and diet. These data suggest that the inuence of adiposity on survival risk is sexually dimorphic and changes with age.

DiscussionData from both study locations suggest that the CR paradigm is effective in delaying the effects of ageing in nonhuman primates but that the age of onset is an important factor in determining the extent to which benecial effects of CR might be induced. In the UW study, reduced bodyweight, reduced adiposity and reduced food intake of the CR monkeys were associated with improved

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survival, with CR monkeys of both sexes surviving longer than controls, B28 and B30 years of age for males and females respectively, and longer than the median age for monkeys in captivity (B26 years of age). Although an impact of CR on survival was not detected within the NIA old-onset cohort, comparison to the UW study shows that bodyweight was signicantly lower in both control and CR groups of males and females than in their UW control counterparts, and was largely equivalent to that of UW CR. All males and females from the NIA old-onset groups consumed fewer calories than their counterpart controls from UW, instead both control and CR were closely aligned with food intake values of UW CR. Importantly, the median survival estimates for old-onset males were very high, similar to what has been reported previously as the 90th percentile for this species (B35 years of age). Six of the original 20 monkeys have lived beyond 40 years of age, the previous maximal lifespan recorded, and one old-onset CR male monkey is currently 43 years old, which is a longevity record for this species. Median survival estimates for old-onset females, B27 and B28 for controls and CR respectively, were also greater than national median lifespan estimates, with one remaining female currently 38 years of age. The clear benet in survival estimates for monkeys within the old-onset cohort compared to UW controls suggests that food intake can and does inuence survival. The lack of difference between control and CR old-onset monkeys suggests that a reduction in food intake beyond that of the controls brings no further advantage. The minimum degree of restriction that confers maximal benet in rhesus monkeys has not yet been identied but is an active topic of investigation. Taken together, data from both UW and NIA studies support the concept that lower food intake in adult or advanced age is associated with improved survival in nonhuman primates.

The interpretation of the outcomes from NIA J/A cohorts is more complicated. The J/A cohorts reveal a sex-dependence in the relationship between bodyweight and food intake that was not observed in the UW cohorts. The NIA J/A CR males consumed signicantly fewer calories than controls for most of the study, and this was reected in a signicantly lower bodyweight and a modest reduction in adiposity. Bodyweight of the NIA J/A control males was in between that of UW controls and CR monkeys even though caloric intake was equivalent between UW and NIA control animals, and age-adjusted average adiposity of NIA J/A males was equivalent to that of UW CR males. Median survival estimates for the NIA J/A male controls and CR were not statistically different at B29 and B26 years, respectively, but estimates for the J/A controls were numerically equivalent to UW CR. These data suggest that in the J/A male cohort, the NIA diet is associated with lower bodyweight, lower adiposity and improved survival in the absence of CR, and that no further advantage is gained by lowering food intake or bodyweight below that of controls. A different picture emerges from the data from NIA J/A females. The CR monkeys consumed signicantly less food than controls for much of the study, but there was no apparent difference in bodyweight or adiposity between them. Median survival estimates for control and CR J/A females were not statistically different from each other at B26 and B23 years respectively, and each were lower than that observed for UW CR. The lack of response in bodyweight to differences in food intake in the female monkeys suggests that there is sexual dimorphism in the relationship between food intake and bodyweight in young animals and in how these parameters relate to median survival. Finally, there is a suggestion that nonhuman primates differ from rodents in the suitability of early onset CR. In rodents, early onset CR is more effective in extending longevity than adult onset CR. For nonhuman primates it appears that CR, while benecial when implemented in adulthood, does not improve survival when

a ControlCR

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Figure 6 | Morbidity curves for monkeys at NIA and UW shown.(a) Graphs represent the rst occurrence of any age-related disease, disorder or condition for combined males and females from UW (top) and NIA J/A (bottom). Statistics related to this gure are provided in Supplementary Information, Supplementary Table 4. (b) Incidence of prevalent age-related conditions in nonhuman primates for control and CR animals from UW and NIA (J/A and old-onset combined). To compare studies, cancer and cardiovascular disorders are reported as incidence upon necropsy and are expressed as a percentage of the animals that are deceased.

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implemented in juveniles. Furthermore, it appears that CR is not uniformly tolerated in young animals, where the NIA J/A CR males and females reached 80% mortality earlier than controls. Although the numerical values for estimated survival were lower in CR groups than in controls, the impact of juvenile onset CR on survival remains undetermined, as the mortality curves for both NIA J/A cohorts are incomplete.

Data from both NIA and UW studies highlight sex-dependent differences in nutritional modulation of body composition and glucoregulatory function, and prompt further investigation of sex-specicity in the connection between nutrition and disease risk. In rodent studies sexual dimorphism in the response to diet-induced metabolic dysfunction has been reported where males were more vulnerable than females to increased adiposity and glucoregulatory dysfunction41. It is perhaps not surprising then that the separation in fasting glucose curves between UW controls and CR was observed in males but not females, and that females, despite being on the same diet as males, were not different in glucose levels between control and CR until after 20 years of age. Although both NIA and UW diets contain equivalent relative amounts of carbohydrate, the sucrose content of the puried UW diet is six times higher than the naturally sourced NIA diet, and the fat content of the UW diet is twice that of the NIA diet. The implication is that elevated sucrose and increased fat content could lead to increased adiposity, negatively impacting glucoregulatory function. All the NIA J/A monkeys maintained adiposity at less than 25% and were in large part euglycemic through middle age. As might be predicted, the UW control males and females had signicantly greater adiposity compared to NIA controls. In the case of UW males, increased adiposity was signicantly associated with higher fasting glucose levels; however, the association did not hold for the UW female monkeys where despite differences in adiposity between control and CR, fasting glucose levels were equivalent through middle age, and multilevel analysis failed to identify a signicant association between adiposity and fasting glucose. Furthermore, there is the suggestion that the effect of diet composition is dependent on intake levels. The UW CR male monkeys, on the same diet as controls but under food limiting conditions, had low adiposity and normal fasting glucose. These data demonstrate that the relationship between adiposity and glucoregulatory impairment is sex-specic, and the relationship between dietary composition and glucoregulatory impairment is dependent on the level of food intake. The signicant relationship between adiposity and survival detected in the UW female cohort also points to sex-dimorphism in the impact of age and the inuence of body composition on mortality risk. This relationship was identied for UW females only, presumably because there was a greater range in levels of adiposity among controls and CR for the UW females than for NIA females where adiposity of monkeys from either diet at either age of onset did not differ. The positive relationship between adiposity and survival in UW females was identied only after correcting for age and diet, suggesting that the interactions among health, body composition and morbidity are sensitive to age and, given the lack of association in the male cohort, sex-dependent.

The catalogue of pathologies identied in aged monkeys is shared with aged humans. The denitions used to identify morbidity were determined by veterinary staff and were essentially equivalent at both sites. A shared feature of both studies is the benecial effect of CR in lowering the risk for age-related morbidity by more than two-fold. Factors contributing to these analyses include a range of conditions that are highly prevalent in human geriatric populations, such as sarcopenia, osteoporosis and arthritis. The benecial effects extended to diseases that are among the most prevalent in human clinical care

including cancer, cardiovascular disease and parameters associated with diabetes. A lower incidence of cancer was one of the rst health benets of CR documented and is considered to be a hallmark of CR in rodents11. The incidence of cancer was lower in CR monkeys at both locations indicating that tumour suppression is a conserved feature of mammalian CR. CR also lowered the incidence of cardiovascular disorders at UW, and NIA monkeys from either diet group appear to have been protected compared to UW Control monkeys. The data shown are based on pathologies identied at necropsy so that nal incidence of both disorders at NIA has yet to be determined. The impact of CR to sustain glucoregulatory function during ageing was a further commonality between the studies. Monkeys presenting with insulin resistance at UW were clinically treated to prevent transition to diabetes, prohibiting a direct comparison between the studies. Although the diagnosis employed at the two study locations represent different time frames in disease progression, the outcome of lower incidence of insulin resistance at UW and lower incidence of diabetes at NIA are very much in agreement. Given the obvious parallels between human and rhesus monkey, it seems highly likely that the benecial effects of CR would also be observed in humans. Reports from the multicenter CALERIE study of short-term CR in humans document changes in bodyweight, body composition, glucoregulatory function and serum risk factors for cardiovascular disease in response to CR (refs 4247). These outcomes in humans align well with reports on rhesus monkey CR (refs 4851), conrming that the primary response to CR is conserved between these two species, and suggesting that the underlying mechanisms may also be conserved.

In conclusion, the NIA and UW nonhuman primate ageing and CR studies address a central concept of relevance to human ageing and human health: that the age-related increase in disease vulnerability in primates is malleable and that ageing itself presents a reasonable target for intervention. The last two decades have seen considerable advances in ageing research in short-lived species and investigations of the mechanisms of CR have been prominent in this work. It will be particularly informative to determine the degree to which consensus hallmarks of ageing described in recent publications52,53 also manifest in primate ageing. The tissues and longitudinal data stored over the course of these two highly controlled monkey studies present a unique resource that can be used to identify key pathways responsive to CR in primates, to uncover primate-specic aspects of the basic biology of ageing, and to determine molecular basis for nutritional modulation of health and ageing. Processes impacted by CR would be prime targets for the development of clinical interventions to offset age-related morbidity, and identication of factors involved in the mechanisms of CR will be pivotal in bringing these ideas to clinical research and human health care.

Methods

Animal care. Specics of housing and animal care have been described in detail elsewhere20,21. Briey, all animals are maintained according to the provisionsof the Animal Welfare Act of 1966 (Public Law 89544), plus its subsequent amendments, as well as the standards set forth in the document entitled The Guide for the Care and Use of Laboratory Animals (NIH Publication No. 8523). Animals rooms are maintained atB21 C and 5065% humidity. Room lighting is automatically controlled on a 12-h light, 12-h dark schedule. Animals are monitored daily for general health by dedicated animal care and research staff and routinely by veterinary staff. Studies at UW and NIA were conducted in accordance with protocols approved respectively by the University of WisconsinMadison Graduate School and National Institute on Aging Institutional Animal Care and Use Committees.

Statistical analysis. The mortality analysis included all animals with known diagnoses or cause of death prior to July 2015. For time-to-event outcomes, the

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three most common methods were used: Kaplan-Meier product-limit method; Cox proportional hazard regression and parametric survival analysis assuming Weibull distributions30. Analyses were separated by site (UW; NIA), age-of-onset and sex. For both Kaplan-Meier and Cox proportional hazards (PH) regression, diet group (CR or control) was used as a predictor to test the effect of CR on mortality and morbidity (the onset of age-associated disease). Cox models were also used to estimate the HRs and 95% CIs. For the mortality analyses, age at death or current age for censored values was used as the time variable. Estimated median and mean lifespans were calculated using both Kaplan-Meier analysis and parametric survival analysis assuming a Weibull distribution. Bodyweight and food intake data were analysed using mixed linear models to account for dependency in the observations due to repeatedly measuring each animal over time. In the morbidity analyses, the age at which the animal experienced its rst age-associated diagnosis was used as the time variable. Animals that had had not experienced an age-associated disease but died of an age-related cause were not censored and their age at death was used as the time variable. For the censored cases where the monkeys were alive and had not experienced an age-associated disease, current age was used as the time variable. For censored cases where monkeys had died but had not experienced an age-associated disease, age at death was used as the time variable. For all Cox models, the PH assumption was tested by rst tting a non-PH Cox regression with a CR by time interaction. The CR by time interactions were not signicant in any model; thus, PH models were considered valid. All analyses were performed in SAS 9.3. The statistical signicance level was set ata 0.05 for all analyses with no adjustments54. Multilevel modelling was

conducted to explore relationships among parameters.

Data availability. The data sets analysed during the current study are available from the corresponding authors on reasonable request.

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Acknowledgements

Thanks to animal care and research staff at both facilities including Scott Baum, Julie

Adriansjach, Saverio Capuano, Casey Fitz, Heather Simmons at UW, and Rick Herbert,

Edward Tilmont, Kelli Vaughan, Mark Szarowicz, Amanda Evans, Danielle Sedlak, Mark

Bryant and Matt Starost at NIA for their tireless efforts and stalwart commitment over

the years. Funding for the UW study was provided by NIH grants P01AG011915,

R01AG040178, R01AG037000, and the Department of Medicine, School of Medicine and

Public Health UW Madison. This publication was made possible in part by NCRR/ORIP

grants P51RR000167/P51OD011106 to the Wisconsin National Primate Research

Center, University of Wisconsin-Madison and the use of resources and facilities at the

William S. Middleton Memorial Veterans Hospital, Madison, WI. The internet Primate

Aging Database is funded by contract HHSN-263-2013-00026C from the NIA. The NIA

study was supported in part by the Intramural Research Program of the NIH, National

Institute on Aging.

Author contributions

J.A.M., R.J.C., J.W.K., G.S.R., D.K.I., R.W., R.d.C. and R.M.A. devised the approach,

long-term statistical support was provided by D.B.A. and T.M.B., T.M.B. conducted the

statistical analysis, J.A.M., R.J.C., R.d.C. and R.M.A. wrote the paper.

Additional information

Supplementary Information accompanies this paper at http://www.nature.com/naturecommunications

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Competing nancial interests: R.W. is a member of the board of LifeGen Technologies,

a company focused on nutritional genomics. G.S.R. is Chief Executive Ofcer of

GeroScience, Inc. and Vice President of Prolongevity Technologies. D.K.I. serves as Chief

Scientic Ofcer for GeroScience, Inc., and Prolongevity Technologies, Inc. D.B.A. serves

on the board of IKEA and has received consulting fees from multiple government, not for

prot, and for prot organizations with interests in obesity and nutrition. None of these

activities benet directly from this research and no competing nancial interests are

declared. The remaining authors declare no competing nancial interests. Reprints and permission information is available online at http://npg.nature.com/reprintsandpermissions/

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How to cite this article: Mattison, J. A. et al. Caloric restriction improves health

and survival of rhesus monkeys. Nat. Commun. 8, 14063 doi: 10.1038/ncomms14063

(2017).

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r The Author(s) 2017

12 NATURE COMMUNICATIONS | 8:14063 | DOI: 10.1038/ncomms14063 | http://www.nature.com/naturecommunications

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Abstract

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Caloric restriction (CR) without malnutrition extends lifespan and delays the onset of age-related disorders in most species but its impact in nonhuman primates has been controversial. In the late 1980s two parallel studies were initiated to determine the effect of CR in rhesus monkeys. The University of Wisconsin study reported a significant positive impact of CR on survival, but the National Institute on Aging study detected no significant survival effect. Here we present a direct comparison of longitudinal data from both studies including survival, bodyweight, food intake, fasting glucose levels and age-related morbidity. We describe differences in study design that could contribute to differences in outcomes, and we report species specificity in the impact of CR in terms of optimal onset and diet. Taken together these data confirm that health benefits of CR are conserved in monkeys and suggest that CR mechanisms are likely translatable to human health.

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Title

Caloric restriction improves health and survival of rhesus monkeys

Author

Mattison, Julie A; Colman, Ricki J; Beasley, T Mark; Allison, David B; Kemnitz, Joseph W; Roth, George S; Ingram, Donald K; Weindruch, Richard; De Cabo, Rafael; Anderson, Rozalyn M

Pages

14063

Publication year

2017

Publication date

Jan 2017

Publisher

Nature Publishing Group

e-ISSN

20411723

Source type

Scholarly Journal

Language of publication

English

DOI

https://doi.org/10.1038/ncomms14063

ProQuest document ID

1859397926

Caloric restriction improves health and survival of rhesus monkeys

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