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Abstract
Aim: The aim of this study was to assess the anti-fungal activity of green coconut water (Cocos nucifera L.) extract on Candida albicans.
Materials and Methods: Extract from (Cocos nucifera L.) was lyophilized and its anti-fungal activity against C. albicans was tested in vitro at various concentrations including 1000 pg, 500 pg, 250 pg and 100 pg respectively. The inhibitory effect of Cocos nucifera L extract on C. albicans was determined by agar culture and applied broth dilution test.
Results: Cocos nucifera L.extract at 1000 pg/ml concentration effectively inhibited the growth of C. albicans (14 ± 0.3) compared with the positive control -amphotericin B (15 ± 0.3) in a dose-dependent manner.
Conclusion: Tender coconut water (Cocos nucifera L.) extract had a strong dose-dependent anti-fu ngal effect on C. albicans.
Key words: Anti-fungal activity, coconut water, Candida albicans
INTRODUCTION
Candidiasis is a widespread oral and perioral opportunistic infection that usually results from overgrowth of endogenous Candida fungal microorganisms. Though many species of Candida exists, C. albicans is the most frequently (>80%) encountered organism in the ambulatory general practice dental patient. It is clinically presented in various forms such as angular chelitis, denture stomatitis, hyperplastic (candidial leukoplakia), chronic atrophic (erythematous), median rhomboid glossitis, inflammatory papillary hyperplasia, mucocutaneous, and pseudomembranous [1,2]. The increasing prevalence of acquired immune deficiency syndrome and other immuno-compromised patient groups in the society, the amplified usage of broad-spectrum antibiotics and corticosteroids, diabetes mellitus, and severe nutritional deficiencies have resulted in the revival of oral candidiasis as a relatively common affliction in patients [3]. Due to the increased resistance and adverse drug reactions to the currently marketed over the counter anti-fungal agents, there is a constant demand for a safe and competent alternative option for the prevention and treatment of these infections. Hence, phytochemicals derived from plants and its products used as traditional medicines are considered as an ideal choice.
The green coconut water (Cocos nucifera L.), a refreshing and nutritious beverage is widely consumed due to its commendable health benefits from control of hypertension to protection against myocardial infarction. [4]. It is assumed that coconut water could be used as an important alternative for oral rehydration and even so for intravenous hydration of patients in remote regions [4]. Besides its nutritional...