Base editing on the rise

Abstract

The ability to mutate a specific base pair in the large genomes of higher eukaryotes is a long-standing goal in the genome engineering community. Recent interest has focused on synthetic ribonucleoproteins known as base editors1,2fusions of a catalytically inactive CRISPRCas9 domain and a cytosine deaminase domain that enable targeted conversion of G-C base pairs to A-T base pairs.