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About the Authors:
Dewi Megawati
Contributed equally to this work with: Dewi Megawati, Sri Masyeni
Affiliation: Faculty of Medicine and Health Sciences, Warmadewa University, Jl. Terompong No. 24 Denpasar, Bali, Indonesia
Sri Masyeni
Contributed equally to this work with: Dewi Megawati, Sri Masyeni
Affiliation: Faculty of Medicine and Health Sciences, Warmadewa University, Jl. Terompong No. 24 Denpasar, Bali, Indonesia
Benediktus Yohan
Affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69, Jakarta, Indonesia
Asri Lestarini
Affiliation: Faculty of Medicine and Health Sciences, Warmadewa University, Jl. Terompong No. 24 Denpasar, Bali, Indonesia
Rahma F. Hayati
Affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69, Jakarta, Indonesia
Febrina Meutiawati
Affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69, Jakarta, Indonesia
Ketut Suryana
Affiliation: Wangaya General Hospital. Jl. Kartini No 133 Denpasar, Bali, Indonesia
Tangking Widarsa
Affiliation: Faculty of Medicine and Health Sciences, Warmadewa University, Jl. Terompong No. 24 Denpasar, Bali, Indonesia
Dewa G. Budiyasa
Affiliation: Sanjiwani General Hospital. Jl. Ciung Wenara No 2 Gianyar, Bali, Indonesia
Ngurah Budiyasa
Affiliation: Wangaya General Hospital. Jl. Kartini No 133 Denpasar, Bali, Indonesia
Khin S. A. Myint
Affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69, Jakarta, Indonesia
R. Tedjo Sasmono
* E-mail: [email protected]
Affiliation: Eijkman Institute for Molecular Biology, Jl. Diponegoro No. 69, Jakarta, Indonesia
ORCID http://orcid.org/0000-0003-0986-2590Abstract
A high number of dengue cases are reported annually in Bali. Despite the endemicity, limited data on dengue is available for Bali localities. Molecular surveillance study was conducted to explore the clinical and virological characteristics of dengue patients in urban Denpasar and rural Gianyar areas in Bali during the peak season in 2015. A total of 205 adult dengue-suspected patients were recruited in a prospective cross-sectional study. Demographic and clinical information were obtained, and dengue screening was performed using NS1 and IgM/IgG ELISAs. Viral RNA was subsequently extracted from patients’ sera for serotyping using conventional RT-PCR and Simplexa Dengue real-time RT-PCR, followed by genotyping with sequencing method. We confirmed 161 patients as having dengue by NS1 and RT-PCR. Among 154 samples successfully serotyped, the DENV-3 was predominant, followed by DENV-1, DENV-2, and DENV-4. Serotype predominance was different between Denpasar and Gianyar. Genotyping results classify DENV-1 isolates into Genotype I and DENV-2 as Cosmopolitan Genotype. The classification grouped isolates into Genotype...