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ABSTRACT
Purpose: We tested the antifungal activity of N,N-phenyl-1,2,3,4-thiatriazole-5-yl-2,4-b-resorcyl-carbothioamide (PTR) ; n-3-(1,2,4-dithiazole-5-thione)-β-resorcylcarbothioamide (DTRTA) ; N,N-phenyl-1,2,3,4-thiatriazol-5-yl-2,4-b-resorcyl-carbothioamide (PHARA) against Candida albicans strains in vitro.
Materials and methods: We synthesized PTR, DTRTA and PHARA at the Department of Chemistry, University of Agriculture in Lublin. We tested the selected three samples with the lowest value of MIC - PTR, DTRTA and PHARA. A reference strain of C. albicans ATCC 10231 and 250 strains of C. albicans isolated from patients were used. Enzymatic activity of the yeast-like fungi was performed by API ZYM test (bioMériux).
Results: The mean MIC C. albicans ATCC 10231 on Sabouraud's Medium was 12.5 mg/L, and YNB Medium and RPMI medium - 6.25 mg/L. The mean MIC C. albicans on Sabouraud's Medium - exposure to PTR - 19.77 mg/L; exposure to DTRTA - 21.06 mg/L, exposure to PHARA - 21.54 mg/L; on YNB Medium - exposure to PTR - 17.79 mg/L, exposure to DTRTA - 16.23 mg/l, exposure to PHARA - 18.92 mg/L; and RPMI Medium - exposure to PTR - 12.73 mg/L, exposure to DTRTA -10.93 mg/l, exposure to PHARA - 10.65 mg/L. The reference C. albicans strain ATCC 10231 had 5 enzymes inhibited - exposure to PTR inhibited the enzymatic activity of 13 enzymes, exposure to DTRTA inhibited the enzymatic activity of 10 enzymes, and exposure to PHARA inhibited the enzymatic activity of 13 enzymes. The C. albicans isolates had 3 enzymes inhibited - after exposure to PTR - 5 enzymes were inhibited, exposure to DTRTA - 9 enzymes were inhibited, and exposure to PHARA - 4 enzymes were inhibited.
Conclusion: The synthesized compounds PTR, DTRA and PHARA exert a moderate antifungal activity against C. albicans strains in vitro.
Key words: Thiatriazole, antifungal activity, Candida albicans, in vitro
INTRODUCTION
Yeasts are part of normal human flora, and invasive infections arise when barrier leakage or impaired immune function occurs [1]. Candida albicans is a common gastrointestinal flora that causes a wide range of severe manifestations when disseminated into the bloodstream.
Candida albicans and Candida species are common pathogens among micro-organisms isolated in intensive care units. Candidemia and candidiasis are major causes of nosocomial infections linked to a number of risk factors such as venous catheters, antimicrobial therapy, parenteral nutrition or immunosuppressive therapies [2]. In...