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Abstract
Given the more aggressive biology of ER? breast cancers, calycosin treatment may result in better clinical outcomes than other phytoestrogens. [...]we focused on the antitumor effect of isoflavones against ER? breast cancer, the possible differences between ER+ and ER? breast cancer cells after treatment with calycosin, and the role of non-ER-mediated signaling in these effects. In our preliminary screen of lncRNAs in SKBR3 breast cancer cells, we saw that calycosin increased the expression of several lncRNAs, including WDR7-7, TTC21B-AS1, and CTA-384D8.34. Since WDR7-7 showed the greatest average increase in expression, we chose to examine the role of WDR7-7 in breast cancer carcinogenesis. [...]we provide evidence that, in addition to the miR-375-ER? feedback loop in ER+ breast cancer cells, a negative relationship between WDR7-7 and GPR30 exists in both ER+ and ER? breast cancer cells. [...]WDR7-7 and GPR30 may be potential mediators of the anti-cancer activity of calycosin that function alongside traditional estrogen receptors and may serve as additional biological targets in estrogen-sensitive tumors.