Abstract

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

Details

Title
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
Author
Martineau, Magalie 1   VIAFID ORCID Logo  ; Somasundaram, Agila 2 ; Grimm, Jonathan B 3 ; Gruber, Todd D 3 ; Choquet, Daniel 4   VIAFID ORCID Logo  ; Taraska, Justin W 2   VIAFID ORCID Logo  ; Lavis, Luke D 3   VIAFID ORCID Logo  ; Perrais, David 1   VIAFID ORCID Logo 

1  University of Bordeaux, Bordeaux, France; Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France 
2  National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, MD, USA 
3  Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA 
4  University of Bordeaux, Bordeaux, France; Centre National de la Recherche Scientifique, Interdisciplinary Institute for Neuroscience, UMR 5297, Bordeaux, France; Bordeaux Imaging Center, UMS 3420 CNRS, Université de Bordeaux, US 4 INSERM, Bordeaux, France 
Pages
1-10
Publication year
2017
Publication date
Nov 2017
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-017-01752-5
ProQuest document ID
1962258204
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.