GENOME ANNOUNCEMENT
The majority of Salmonella enterica infections recorded in humans are foodborne, with S. enterica serotypes Enteritidis and Typhimurium being the two most reported serovars (1). The incidence of S. enterica serovars differs considerably according to locality, district, region, and country. Hence, detailed surveillance and identification of the prevalent S. enterica serovars in humans and poultry should be implemented to establish a control program for an area. True to its name, S. enterica is associated with intestinal diseases of chickens, turkeys, and ducks worldwide (2). Multiple signs can be observed in the early onset of infection, including dejection, ruffled feathers, closed eyes, diarrhea, vent pasting, and loss of appetite and thirst. Postmortem lesions include enteritis, focal necrotic intestinal lesions, foci in the liver, unabsorbed yolk, cheesy cores in the caeca, and pericarditis (3).
The S. enterica subsp. enterica serovar Typhimurium strain UPM 260 was isolated from a broiler chicken in Perak, Malaysia, in 2014. The strain was isolated from cloacal swabs and identified based on biochemical characterization (4). Currently, there is neither a complete nor a draft genome sequence of S. Typhimurium strain UPM 260 from Malaysia available. The whole-genome sequencing was performed with genomic DNA using paired-end sequencing on the Illumina HiSeq platform. De novo assembly was carried out using CLC Genomics Workbench version 7.5.1 (Qiagen, USA), which generated 107 contigs with a G+C content of 52.0% and a total genome size of 4.9 Mb. The sequence was then annotated using the Rapid Annotations using Subsystems Technology (RAST) server (5). The N50 value was 104,184 bp, and the L50 was reached in 15 contigs. The data provided will empower further studies of the epidemiology of S. Typhimurium strains isolated in Malaysia.
Accession number(s).
This whole-genome shotgun project has been deposited at NCBI GenBank under the accession number NQWC00000000.
b Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
c Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
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Abstract
ABSTRACT
Salmonella enterica subsp. enterica serovar Typhimurium is one of several well-categorized Salmonella serotypes recognized globally. Here, we report the whole-genome sequence of S. Typhimurium strain UPM 260, isolated from a broiler chicken.
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Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer