Content area
Abstract
According to the statistics of Chinese National Office for Cancer Prevention and Control, GC was the 2nd and 3rd commonly diagnosed cancers among men and women respectively, and was the 2nd leading causes of cancer death among both sexes [2]. [...]the transparency of the embryos could facilitate the visualization of tumor cell behaviors and its interactions with the microenvironment such as host blood vessels [22, 35]. [...]the ability of using less patient cell numbers (200-800 cells/embryo vs. 1 million cells/mouse) [36], the efficiency of zPDX model for drug screening right after cell dissociation (cell at passage 0 in zebrafish vs. passage 3 or after in mouse) that preserves a better human origin [13, 18, 37], the power of performing medium throughput in vivo drug screening with a short latency (7 days in zebrafish model vs. several weeks to months in mouse model) that enable quick screening in real time [13, 18]. Compound with Log P values less than 1 are typically not well-absorbed from the medium by zebrafish embryos [42, 43], which might cause the poor absorption of 5-FU by the zebrafish embryo by soaking. [...]we administered 5-FU to the embryos by microinjection. 5 and 50 ?M of 5-FU were prepared in embryo medium, 10 nl of each drug medium was injected to the yolk sac of zebrafish embryo, and 5-FU at 6.5 and 65 ng/embryo caused obvious tumor cell growth regression.