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Abstract Objectives To explore whether the thin layer chromatography (TLC) can be used to guide the preparation of drug containing serum of eucalyptus oil. [Methods] Eucalyptus oil samples with different dilution ratio were detected by TLC. Eucalyptus oil was intragastrically administered to mice, serum samples of different eucalyptus oil doses, different intragastric administration methods arai different blood sampling times were collected. The above samples were detected by TLC, and the above results were verified by gas chromatography. [Results] TLC can detect concentration difference of eucaly ptus oil samples with different dilution ratio. TLC can provide qualitative and semi-quantitative detection of eucalyptus oil in senun. High, medium and low dose of eucalyptus oil in serum had different effects on the growth of MCF-7 cells in the SD rats (P <0.05). [Conclusions] TLC can be used to guide the preparation of drug containing serum of eucalyptus oil.
Key words Chinese medicine drug containing semm. Eucalyptus oil. Thin layer chromatography
1 Introduction
The method of pharmacological experiments with drug containing serum of animal is called a serum pharmacological method. The experimental results obtained by this method can not only reflect the direct effects of the absorbable components in traditional Chinese medicine, bul also reflect the indirect effects of the metabolites formed by the body through the drug components and the endogenous substances induced by the drug, to keep in vitro experimental results consistent with in vivo experimental results to a certain extent. Besides, this method also applied the advantages of strong controllability in vitro experiment, and elaborated the pharmacological mechanism of drugs from cells, protein, and gene level by means of cell and molecular biology technology, so as to realize combination of traditional medicine and modern science. However, when using serum pharmacology to study the pharmacological effects of eucalyptus oil, the following problems need lo be made clear in the preparation of drug containing serum : whether the serum contains the original dmg component, whether there is generation of new product, whether there is significant difference in serum drug concentration between the experimental groups, and whether the scheme for preparation of drug containing serum is suitable. In these aspects, the selection of scheme for preparation of drug containing serum is the key for preparation of drug containing...