Abstract

Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.

Details

Title
Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background
Author
Ghimire, Sabitri 1 ; Margot Van der Jeught 1 ; Neupane, Jitesh 1 ; Roost, Matthias S 2 ; Anckaert, Jasper 3 ; Popovic, Mina 1 ; Filip Van Nieuwerburgh 4   VIAFID ORCID Logo  ; Mestdagh, Pieter 3 ; Vandesompele, Jo 3   VIAFID ORCID Logo  ; Deforce, Dieter 4   VIAFID ORCID Logo  ; Menten, Björn 3 ; Susana Chuva de Sousa Lopes 5   VIAFID ORCID Logo  ; De Sutter, Petra 1 ; Heindryckx, Björn 1 

1  Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium 
2  Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands 
3  Department of Pediatrics and Medical Genetics, Ghent University Hospital, Gent, Belgium 
4  Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium 
5  Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands 
Pages
1-11
Publication year
2018
Publication date
Apr 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-24051-5
ProQuest document ID
2024481722
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.