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About the Authors:
Thomas Hennig
Contributed equally to this work with: Thomas Hennig, Marco Michalski
Roles Investigation, Writing - review & editing
Affiliation: Institut für Virologie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
Marco Michalski
Contributed equally to this work with: Thomas Hennig, Marco Michalski
Roles Investigation
Affiliation: The Babraham Institute, Cambridge, United Kingdom
Andrzej J. Rutkowski
Roles Investigation, Writing - review & editing
Affiliation: Division of Infectious Diseases, Department of Medicine, University of Cambridge, Cambridge, United Kingdom
Lara Djakovic
Roles Investigation
Affiliation: Institut für Virologie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
ORCID http://orcid.org/0000-0002-9368-9403
Adam W. Whisnant
Roles Investigation
Affiliation: Institut für Virologie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
ORCID http://orcid.org/0000-0003-2039-2809
Marie-Sophie Friedl
Roles Formal analysis, Software, Writing - review & editing
Affiliation: Institut für Informatik, Ludwig-Maximilians-Universität München, München, Germany
Bhaskar Anand Jha
Roles Investigation
Affiliation: Institut für Virologie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
Marisa A. P. Baptista
Roles Investigation
Affiliation: Institut für Virologie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
Anne L’Hernault
Roles Investigation
Affiliation: Division of Infectious Diseases, Department of Medicine, University of Cambridge, Cambridge, United Kingdom
Florian Erhard
Roles Methodology, Writing - review & editing
Affiliation: Institut für Virologie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
ORCID http://orcid.org/0000-0002-3574-6983
Lars Dölken
Roles Conceptualization, Funding acquisition, Methodology, Supervision, Visualization, Writing - original draft, Writing - review & editing
* E-mail: [email protected] (LD); [email protected] (CCF)
Affiliations Institut für Virologie, Julius-Maximilians-Universität Würzburg, Würzburg, Germany, Division of Infectious Diseases, Department of Medicine, University of Cambridge, Cambridge, United Kingdom
Caroline C. Friedel
Roles Formal analysis, Funding acquisition, Methodology, Software, Supervision, Visualization, Writing - original draft, Writing - review & editing
* E-mail: [email protected] (LD); [email protected] (CCF)
Affiliation: Institut für Informatik, Ludwig-Maximilians-Universität München, München, Germany
ORCID http://orcid.org/0000-0003-3569-4877Abstract
Lytic herpes simplex virus 1 (HSV-1) infection triggers disruption of transcription termination (DoTT) of most cellular genes, resulting in extensive intergenic transcription. Similarly, cellular stress responses lead to gene-specific transcription downstream of genes (DoG). In this study, we performed a detailed comparison of DoTT/DoG transcription between HSV-1 infection, salt and heat stress in primary human fibroblasts using 4sU-seq and ATAC-seq. Although DoTT at late times of HSV-1 infection was substantially more prominent than DoG transcription in salt and heat stress, poly(A) read-through due to DoTT/DoG transcription and affected genes were significantly correlated between all three conditions, in particular at earlier times of infection. We speculate that...