Nucleus accumbens controls wakefulness by a subpopulation of neurons expressing dopamine D1 receptors

Abstract

Nucleus accumbens (NAc) is involved in behaviors that depend on heightened wakefulness, but its impact on arousal remains unclear. Here, we demonstrate that NAc dopamine D₁ receptor (D₁R)-expressing neurons are essential for behavioral arousal. Using in vivo fiber photometry in mice, we find arousal-dependent increases in population activity of NAc D₁R neurons. Optogenetic activation of NAc D₁R neurons induces immediate transitions from non-rapid eye movement sleep to wakefulness, and chemogenetic stimulation prolongs arousal, with decreased food intake. Patch-clamp, tracing, immunohistochemistry, and electron microscopy reveal that NAc D₁R neurons project to the midbrain and lateral hypothalamus, and might disinhibit midbrain dopamine neurons and lateral hypothalamus orexin neurons. Photoactivation of terminals in the midbrain and lateral hypothalamus is sufficient to induce wakefulness. Silencing of NAc D₁R neurons suppresses arousal, with increased nest-building behaviors. Collectively, our data indicate that NAc D₁R neuron circuits are essential for the induction and maintenance of wakefulness.