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Abstract:
Biosurfactant are amphiphilic compound have been progressively more attracting interest of the scientific community as promising candidates for the replacement of a number of synthetic surfactants. Saccharomyces cerevisiae (3) was isolated from imported dry yeast which were available in local market.extracted mannoproteins with emulsification properties from the cell walls of S. cerevisiae (3) cultivated in YPEG medium by three step to purification; in the present study, cell wall mannoproteins of intact yeast were purified using a simple treatment of yeast with mercaptoethanol and sodium dodecyl sulfate followed by ion exchange chromatography and by gel filteration chromatography. The pure mannoprotein was extracted display emulsion activity of 80% towards Kerosin oil as oil-in-water, while the surface activity was 37mm . The molecular weight of manoprotein was determined, where was 89.1 KD. the pure biosurfactant had higher inhibitor activity against Corynebacterium urealyticum , than partially purified where hieghst diameter of inhibition zone was (18mm).
Introduction :
S.cerevisiae is a model organism that is generally referred to as baker's yeast (Duina et al, 2014). It was the first eukaryotic organism whose genome was fully sequenced in 1996,it became a promising model for many studies with its simple genome and short growth period (Yılmaz et al.,2012).Belong to the kingdom of Fungi, class; Ascomycetes, phylum (Ascomycota ), family Saccharomycetaceae (Linder, 2012). S. cerevisiae is a single cell organism that contains a nucleus and other membrane organelles.(Dickinson and Schweizer, 2004).
Saccharomyces cerevisiae comes in the forefront of these microorganisms that have been fully exploited for benefit from it. (Mohamudha and Aye sha ,2010),these substances exhibit a variety of useful properties like, antimicrobial and antiadhesive agents (Johny, 2013), and could have some applications in the food and cosmetics industries. S. cerevisiae have ability to produce compounds called biosurfactant (Dikit et al, 2010).
Biosurfactants is biological surface active compounds produced by living cells microorganisms, mainly bacteria, fungi and yeasts (Płaza et al., 2014). They are produced on microbial cell surfaces or are extracellularly secreted (Salmanet al.,2016), which contains both hydrophobic and hydrophilic moieties (Vijayakumar et al.,2016) that confer the ability to accumulate between fluid phases and thus it is used in reduction of interfacial tension (Kalyani et al., 2011).
Corynebacterium urealyticum (previously known as the coryneform CDC 97 group D2) , was...