Abstract

There is a growing necessity to acquire more profound knowledge on the quantity of eluates from resin-based dental materials, especially with regard to bisphenol A (BPA). The aim of the present study was to develop a highly sensitive method to characterize the short-term release of BPA in saliva with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), using an extraction step and additional derivatization of BPA with pyridine-3-sulfonyl chloride. Light-cured resin-based composites were incubated at 37 °C in 1 mL artificial saliva, which was refreshed daily for one week. The final protocol allows accurate quantification of very low levels of BPA in samples of artificial saliva (i.e. 1.10 pmol BPA/mL or 250 pg/mL). The daily BPA-release from dental composites, ranging from 1.10 to 7.46 pmol BPA/mL, was characterized over a period of 7 days. The highest total amount of BPA was released from Solitaire 2 (24.72 ± 2.86 pmol), followed by G-ænial Posterior (15.51 ± 0.88 pmol) and Filtek Supreme XTE (12.00 ± 1.31 pmol). In contrast, only trace amounts of BPA were released from Ceram.x Universal. This UPLC-MS/MS method might be used for clinical research focusing on the evaluation of the clinical relevance of BPA release from dental materials.

Details

Title
A novel high sensitivity UPLC-MS/MS method for the evaluation of bisphenol A leaching from dental materials
Author
Siemon De Nys 1   VIAFID ORCID Logo  ; Putzeys, Eveline 1 ; Vervliet, Philippe 2   VIAFID ORCID Logo  ; Covaci, Adrian 2 ; Boonen, Imke 3 ; Elskens, Marc 3 ; Vanoirbeek, Jeroen 4   VIAFID ORCID Logo  ; Godderis, Lode 5 ; Bart Van Meerbeek 1 ; Van Landuyt, Kirsten L 1 ; Duca, Radu Corneliu 4 

1  KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven (UZ Leuven), Dentistry, Leuven, Belgium 
2  Toxicological Centre, University of Antwerp, Wilrijk, Belgium 
3  Department of Analytical, Environmental and Geo-Chemistry, Vrije Universiteit Brussel, Ixelles, Belgium 
4  Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium 
5  Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium; IDEWE, External service for prevention and protection at work, Heverlee, Belgium 
Pages
1-7
Publication year
2018
Publication date
May 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-24815-z
ProQuest document ID
2034271452
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.