Abstract

Many reports have demonstrated that radiation stimulates reactive oxygen species (ROS) production by mitochondria for a few hours to a few days after irradiation. However, these studies were performed using cell lines, and there is a lack of information about redox homeostasis in irradiated animals and humans. Blood redox homeostasis reflects the body condition well and can be used as a diagnostic marker. However, most redox homeostasis studies have focused on plasma or serum, and the anti-oxidant capacity of whole blood has scarcely been investigated. Here, we report changes in the anti-oxidant capacity of whole blood after X-ray irradiation using C57BL/6 J mice. Whole-blood anti-oxidant capacity was measured by electron spin resonance (ESR) spin trapping using a novel spin-trapping agent, 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (DPhPMPO). We found that whole-blood anti-oxidant capacity decreased in a dose-dependent manner (correlation factor, r > 0.9; P < 0.05) from 2 to 24 days after irradiation with 0.5–3 Gy. We further found that the red blood cell (RBC) glutathione level decreased and lipid peroxidation level increased in a dose-dependent manner from 2 to 6 days after irradiation. These findings suggest that blood redox state may be a useful biomarker for estimating exposure doses during nuclear and/or radiation accidents.

Details

Title
Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
Author
Sun, Lue 1   VIAFID ORCID Logo  ; Inaba, Yohei 2 ; Sato, Keizo 3 ; Hirayama, Aki 4 ; Tsuboi, Koji 5 ; Okazaki, Ryuji 6 ; Chida, Koichi 2 ; Moritake, Takashi 6 

 Department of Radiation Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan; Department of Radiological Health Science, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, Kitakyushu, Fukuoka, Japan 
 Course of Radiological Technology, Health Sciences, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan; Department of Radiation Disaster Medicine, International Research Institute of Disaster Science, Tohoku University, Aoba-ku, Sendai, Japan 
 School of Pharmacy, Kyushu University of Health and Welfare, Miyazaki, Japan 
 Center for Integrative Medicine, Tsukuba University of Technology, Tsukuba, Japan 
 Department of Radiation Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan 
 Department of Radiological Health Science, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, Kitakyushu, Fukuoka, Japan 
Pages
1-8
Publication year
2018
Publication date
May 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2036771037
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.