Association assessment of Interleukine-10 gene polymorphism and its expression status with susceptibility to coronary artery disease in Iran

Abstract

Background

The cytokines are potent inflammatory factors that regulate each stage of atherosclerosis leading to the disease development. Interleukin-10 (IL-10) as an anti-inflammatory cytokine can develop atherosclerosis by inhibiting the synthesis of metalloproteinase. Moreover, IL-10 promotes the plaque stability by preserving the extracellular matrix and fibrous cap.

Aim

We evaluated the association of the two IL-10 promoter gene polymorphisms with susceptibility to coronary artery disease (CAD) in Iranian population.

Subjects and methods

We used the Sequence Specific Primer-Polymerase Chain Reaction method to determine genotypes. We also studied mRNA expression of the IL-10 gene in Iranian CAD patients using quantitative real-time PCR.

Results

There was a significant association between IL-10(−819) T allele and IL-10(−819) T/T genotype, and CAD (p=0.041 and p=0.042 respectively). There also was a significant association between IL-10(−1082) G allele and IL-10(−1082) G/G genotype, and CAD (p=0.017 and p=0.020 respectively). Genotype T/T of IL-10(−819) polymorphism significantly associated with the two vessel disease type (p=0.017). In addition, there was a significant association between IL-10(−1082) G/G genotype and the three vessel disease type (p=0.009). IL-10 mRNA expression was significantly decreased 3.36-fold in samples with IL-10(−819) polymorphism and 1.98-fold in individuals with IL-10(−1082) polymorphism.

Conclusions

Our results suggest that IL-10 gene promoter polymorphisms may influence both coronary artery disease risks and severity in Iranian patients.