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About the Authors:
Anita Murer
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing - original draft
Affiliation: Viral Immunobiology, Institute of Experimental Immunology, University of Zürich, Zürich, Switzerland
Donal McHugh
Roles Data curation, Investigation, Writing - review & editing
Affiliation: Viral Immunobiology, Institute of Experimental Immunology, University of Zürich, Zürich, Switzerland
Nicole Caduff
Roles Data curation, Investigation, Methodology, Writing - review & editing
Affiliation: Viral Immunobiology, Institute of Experimental Immunology, University of Zürich, Zürich, Switzerland
Jens Kalchschmidt
Roles Methodology, Resources, Writing - review & editing
Affiliation: Genomics and Immunity, NIAMS, National Institutes of Health, Bethesda, MD, United States of America
Mario Barros
Roles Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Affiliation: Institute of Pathology, Unfallkrankenhaus Berlin, Berlin, Germany
Andrea Zbinden
Roles Data curation, Methodology, Resources, Writing - review & editing
Affiliation: Institute of Medical Virology, University of Zürich, Zürich, Switzerland
Riccarda Capaul
Roles Data curation, Methodology, Resources, Writing - review & editing
Affiliation: Institute of Medical Virology, University of Zürich, Zürich, Switzerland
ORCID http://orcid.org/0000-0003-2737-0354
Gerald Niedobitek
Roles Funding acquisition, Supervision, Writing - review & editing
Affiliation: Institute of Pathology, Unfallkrankenhaus Berlin, Berlin, Germany
Martin Allday
Roles Conceptualization, Supervision, Writing - review & editing
Affiliation: Molecular Virology, Department of Medicine, Imperial College London, London, United Kingdom
Obinna Chijioke
Roles Conceptualization, Formal analysis, Supervision, Writing - original draft
Affiliations Viral Immunobiology, Institute of Experimental Immunology, University of Zürich, Zürich, Switzerland, Institute of Pathology and Molecular Pathology, University Hospital Zürich, Zürich, Switzerland
ORCID http://orcid.org/0000-0003-4247-5947
Christian Münz
Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Writing - original draft
* E-mail: [email protected]
Affiliation: Viral Immunobiology, Institute of Experimental Immunology, University of Zürich, Zürich, Switzerland
ORCID http://orcid.org/0000-0001-6419-1940Abstract
The oncogenic Epstein Barr virus (EBV) infects the majority of the human population and usually persists within its host for life without symptoms. The EBV oncoproteins nuclear antigen 3A (EBNA3A) and 3C (EBNA3C) are required for B cell transformation in vitro and are expressed in EBV associated immunoblastic lymphomas in vivo. In order to address the necessity of EBNA3A and EBNA3C for persistent EBV infection in vivo, we infected NOD-scid [gamma]cnull mice with reconstituted human immune system components (huNSG mice) with recombinant EBV mutants devoid of EBNA3A or EBNA3C expression. These EBV mutants established latent infection in...